Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AC2117_RS17095 Genome accession   NZ_LS999521
Coordinates   3573855..3575567 (+) Length   570 a.a.
NCBI ID   WP_133975668.1    Uniprot ID   A0A446ZNS9
Organism   Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3568855..3580567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC2117_RS17060 (AC2117_03382) nusA 3569741..3571225 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  AC2117_RS17065 (AC2117_03383) rimP 3571263..3571787 (-) 525 WP_092710299.1 ribosome maturation factor RimP -
  AC2117_RS17085 (AC2117_03387) secG 3572427..3572756 (-) 330 WP_092710024.1 preprotein translocase subunit SecG -
  AC2117_RS17090 (AC2117_03388) tpiA 3572769..3573563 (-) 795 WP_133975666.1 triose-phosphate isomerase -
  AC2117_RS17095 (AC2117_03389) pilB 3573855..3575567 (+) 1713 WP_133975668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AC2117_RS17100 (AC2117_03390) pilC 3575595..3576821 (+) 1227 WP_133975670.1 type II secretion system F family protein Machinery gene
  AC2117_RS19165 (AC2117_03391) pilD 3576821..3577681 (+) 861 WP_133975672.1 prepilin peptidase Machinery gene
  AC2117_RS17110 (AC2117_03392) coaE 3577683..3578279 (+) 597 WP_133975674.1 dephospho-CoA kinase -
  AC2117_RS17115 (AC2117_03393) - 3578276..3579190 (-) 915 WP_133975676.1 DMT family transporter -
  AC2117_RS17120 (AC2117_03394) rlmB 3579226..3579975 (-) 750 WP_133975678.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AC2117_RS17125 (AC2117_03395) - 3580078..3580404 (-) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63423.76 Da        Isoelectric Point: 4.9634

>NTDB_id=1143506 AC2117_RS17095 WP_133975668.1 3573855..3575567(+) (pilB) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDAVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLVQRGQILYVATSNPSNIEAIDAIRFNSKLMVEAVIVEHHKLEKILAQQFTEESSFEFNDEEF
DLDVDLDTSTTPEEDDDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPTDIPNQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRCSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1143506 AC2117_RS17095 WP_133975668.1 3573855..3575567(+) (pilB) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATGCGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGGTCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAACCCAAGCAATATTGAAGCGATTGATGCTATCCGCTTCAACAGCAAGCTTATGGTTGAAGCGGTTATTG
TTGAACACCATAAACTTGAGAAAATTCTGGCACAGCAATTTACGGAAGAAAGCAGTTTCGAGTTTAATGATGAAGAGTTC
GATCTTGATGTAGATCTTGATACTTCAACCACCCCTGAAGAAGATGATGACACTCCTCAGGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGGGCTTCAGACTTGCATTTCGAACCGTATGAGAAATCGT
ATCGCGTCCGTTATCGGGTCGATGGAGTCTTACGCCAGATTGCAAATCCACCTTTGCAACTCGCCACTCGGTTGGCTTCA
CGTTTAAAAGTTATGTCTCAAATGGACATTTCCGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAGCTCAAGCTATCGAA
AACTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTTGATCCAT
CGAGTGCCATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCGCTCTTTATGGAAGCATTAGATAAACCA
CAAGGTATGCTCTTAATTACTGGTCCTACAGGTTCTGGTAAAACAGTATCTTTATATACGGGCCTCAACATTTTAAATAC
TGAAAGTTCTAATATTTCTACTGCTGAAGATCCAGTGGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAATCCTA
AGGTAGGTCTTACTTTTTCGGCTGCGCTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAGGCTGCTCAGACAGGTCACATGGTGATGTCCACACTACATACCAACAGTGC
CCCTGAAACACTTACTCGTCTACGCAATATGGGAGTTCCATCTTTCAATATTGCGACATCGGTCAATTTGGTTATTGCTC
AACGTTTGGCACGTCGATTATGCCCTCAATGTAAGGTACCTACTGACATTCCCAACCAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGATCTAACACATCCCGATTTTCAAGTTTTTCAACCGGTCGGTTGTCCTGAGTGTCGTGAGGGCTACAAAGG
TCGAGTAGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTACGTTGCTCAGGTTTAAAGAAAGTCATGCAAGGTGTTACTTCG
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A446ZNS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

93.158

100

0.932
  pilB Acinetobacter baylyi ADP1

78.772

100

0.788
  pilB Legionella pneumophila strain ERS1305867

52.381

99.474

0.521
  pilF Neisseria gonorrhoeae MS11

48.754

98.596

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

51.132

92.982

0.475
  pilB Vibrio campbellii strain DS40M4

51.06

91.053

0.465
  pilB Vibrio cholerae strain A1552

49.615

91.228

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

35.831

100

0.386

Multiple sequence alignment