Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D5R51_RS02025 Genome accession   NZ_LS997867
Coordinates   412781..413218 (-) Length   145 a.a.
NCBI ID   WP_119090810.1    Uniprot ID   -
Organism   Vibrio paracholerae strain NCTC 30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407781..418218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5R51_RS02005 (SAMEA104470976_00399) coaE 408279..408887 (-) 609 WP_162891880.1 dephospho-CoA kinase -
  D5R51_RS02010 (SAMEA104470976_00400) pilD 408884..409759 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  D5R51_RS02015 (SAMEA104470976_00401) pilC 409817..411043 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  D5R51_RS02020 (SAMEA104470976_00402) pilB 411086..412774 (-) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D5R51_RS02025 (SAMEA104470976_00403) pilA 412781..413218 (-) 438 WP_119090810.1 type IV pilin protein Machinery gene
  D5R51_RS02030 (SAMEA104470976_00404) nadC 413463..414353 (-) 891 WP_119090811.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D5R51_RS02040 (SAMEA104470976_00405) ampD 414655..415200 (+) 546 WP_119090812.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D5R51_RS02045 (SAMEA104470976_00406) fldB 415469..415987 (-) 519 WP_000690112.1 flavodoxin FldB -
  D5R51_RS02050 (SAMEA104470976_00407) xerD 416125..417033 (+) 909 WP_001287470.1 site-specific tyrosine recombinase XerD -
  D5R51_RS02055 (SAMEA104470976_00408) dsbC 417050..417802 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15259.54 Da        Isoelectric Point: 9.2986

>NTDB_id=1143336 D5R51_RS02025 WP_119090810.1 412781..413218(-) (pilA) [Vibrio paracholerae strain NCTC 30]
MKAYKNKQQKGFTLIELMIVVAIIGVLAAVAIPAYKSYVTKSELATGAATLRSLLTNIDMHYQEKGTYVGMGLGDIGASQ
TMSGLGDINLTYLTASTASATFTFDNSSVNSAVIKYSKSATGWVCSITPNQASAVTTETKPKGCN

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1143336 D5R51_RS02025 WP_119090810.1 412781..413218(-) (pilA) [Vibrio paracholerae strain NCTC 30]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGCGTGTT
GGCAGCTGTCGCTATTCCTGCTTACAAAAGCTACGTGACCAAAAGTGAGCTTGCAACAGGTGCAGCAACTTTAAGAAGCC
TGCTGACAAATATTGATATGCACTATCAAGAGAAGGGTACTTATGTTGGCATGGGACTAGGTGATATTGGTGCCTCTCAA
ACGATGAGTGGTTTAGGTGATATCAACTTAACCTACTTAACGGCTAGTACAGCAAGCGCTACTTTCACATTTGATAACTC
TTCAGTAAACAGTGCTGTTATTAAATACAGCAAATCAGCCACTGGATGGGTCTGTTCTATAACACCAAACCAAGCATCCG
CTGTTACAACAGAAACAAAACCTAAAGGCTGTAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.223

100

0.49
  pilA Vibrio cholerae strain A1552

45.223

100

0.49
  pilA Vibrio cholerae C6706

45.223

100

0.49
  pilA Vibrio parahaemolyticus RIMD 2210633

48.819

87.586

0.428
  pilA Pseudomonas aeruginosa PAK

36.601

100

0.386
  pilA Acinetobacter baumannii strain A118

37.589

97.241

0.366

Multiple sequence alignment