Detailed information NTG1143333

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: D5R51_RS02010
• Genome accession: NZ_LS997867
• Coordinates: 408884..409759 (-)
• Length: 291 a.a.
• NCBI ID: WP_000418747.1
• Uniprot ID: H9L4S3
• Organism: Vibrio paracholerae strain NCTC 30
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 403884..414759

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D5R51_RS01990 (SAMEA104470976_00396)	parC	404581..406866 (+)	2286	WP_119090807.1	DNA topoisomerase IV subunit A	-
D5R51_RS01995 (SAMEA104470976_00397)	yacG	407153..407350 (-)	198	WP_000162865.1	DNA gyrase inhibitor YacG	-
D5R51_RS02000 (SAMEA104470976_00398)	zapD	407507..408247 (-)	741	WP_119090808.1	cell division protein ZapD	-
D5R51_RS02005 (SAMEA104470976_00399)	coaE	408279..408887 (-)	609	WP_162891880.1	dephospho-CoA kinase	-
D5R51_RS02010 (SAMEA104470976_00400)	pilD	408884..409759 (-)	876	WP_000418747.1	A24 family peptidase	Machinery gene
D5R51_RS02015 (SAMEA104470976_00401)	pilC	409817..411043 (-)	1227	WP_000648506.1	type II secretion system F family protein	Machinery gene
D5R51_RS02020 (SAMEA104470976_00402)	pilB	411086..412774 (-)	1689	WP_000957200.1	type IV-A pilus assembly ATPase PilB	Machinery gene
D5R51_RS02025 (SAMEA104470976_00403)	pilA	412781..413218 (-)	438	WP_119090810.1	type IV pilin protein	Machinery gene
D5R51_RS02030 (SAMEA104470976_00404)	nadC	413463..414353 (-)	891	WP_119090811.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32433.69 Da
• Isoelectric Point: 7.1618

>NTDB_id=1143333 D5R51_RS02010 WP_000418747.1 408884..409759(-) (pilD) [Vibrio paracholerae strain NCTC 30]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide

• Length: 876 bp

>NTDB_id=1143333 D5R51_RS02010 WP_000418747.1 408884..409759(-) (pilD) [Vibrio paracholerae strain NCTC 30]
ATGGAACTGTTTTACTTCTACCCTTGGCTTTTTCCGGTGCTCGCCACCCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATTCCACTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCATTTTCCATTCGGTGTATTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCGACCTTCATCGACTTCGATACCCTGTTGTTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCGCTGGCACTGCTTGGCTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCACTGTGGTCGATTTATTGGCTATTTAAACTGCTGACTGGTAAAGAAGGCATGGGGTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTCGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: H9L4S3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	100	100	1
pilD	Vibrio campbellii strain DS40M4	75.347	98.969	0.746
pilD	Acinetobacter nosocomialis M2	53.676	93.471	0.502
pilD	Acinetobacter baumannii D1279779	53.309	93.471	0.498
pilD	Neisseria gonorrhoeae MS11	44.792	98.969	0.443