Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   R615_RS14940 Genome accession   NZ_CP006829
Coordinates   3287115..3289214 (-) Length   699 a.a.
NCBI ID   WP_025266364.1    Uniprot ID   -
Organism   Thalassolituus oleivorans R6-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3282115..3294214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R615_RS14925 (R615_15230) - 3284020..3285468 (-) 1449 WP_025266361.1 SPOR domain-containing protein -
  R615_RS14930 (R615_15235) aroB 3285486..3286577 (-) 1092 WP_025266362.1 3-dehydroquinate synthase -
  R615_RS14935 (R615_15240) aroK 3286592..3287110 (-) 519 WP_197019469.1 shikimate kinase AroK -
  R615_RS14940 (R615_15245) pilQ 3287115..3289214 (-) 2100 WP_025266364.1 type IV pilus secretin PilQ Machinery gene
  R615_RS14945 (R615_15250) - 3289217..3289756 (-) 540 WP_025266365.1 pilus assembly protein PilP -
  R615_RS14950 (R615_15255) - 3289758..3290396 (-) 639 WP_025266366.1 type 4a pilus biogenesis protein PilO -
  R615_RS14955 (R615_15260) - 3290398..3290952 (-) 555 WP_015488345.1 PilN domain-containing protein -
  R615_RS14960 (R615_15265) pilM 3290952..3292049 (-) 1098 WP_015488346.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75434.88 Da        Isoelectric Point: 4.7659

>NTDB_id=114316 R615_RS14940 WP_025266364.1 3287115..3289214(-) (pilQ) [Thalassolituus oleivorans R6-15]
MKIIKGLRQVVFAVPAMLLLMSADALAVTLKDIKFSTMAGDITEIALEFDGAPPEVSGYTIEKPARIAIDLPGVQSALQT
KRHEIGNGNARSATIVATKDRARLVVNLTQLAAYTTTANGNTLLVRIGERSEEKVFAGNTVTSASGASVSPASDMKVVNV
DFRRGELGEGQIQIMLNDARASANIRREGSKIIADLSGVRLPDVLSRRLDVTDFATPVKYVDARREDGVTRIVIEPTSDN
FEYLAYQTDKLLSINVSEPPKDQKEAQKKQFPFTGEKLSLNFQNIEVRAVLQLIADFTGLNLVASDSVQGAITLRLQNVP
WDQALDLVLKTKGLGQRQMGSVLLIAPAAEIAAREKVELEAVRQVEELAPLVTEYMQLKYAKASVLATLLTSEQGLLSER
GTAVVDERTNTLLMKDTSANLEKVREAIIMLDVPVRQVLIEARIVVANTSVGKELGVKWGGASFKDNGSNWTTAGGSQQT
LTEGSQILFDRATTGSSTQNIDLTNANVVDFGATNAAASTFAIGYQTADFLLDLELSAIETEGRAEIVSQPRVITADGQT
ASIESGTEIPYQQASSSGATNVAFKSAVLRLEVTPQITPDDRIIMDLLINQDSVGEQTSAGPSINTNAVETQVLVENGET
VVLGGIFRSEDVTTIRKTPFFGDLPLIGALFRYKSKTDTKSELLVFITPRLVKDTLSTR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=114316 R615_RS14940 WP_025266364.1 3287115..3289214(-) (pilQ) [Thalassolituus oleivorans R6-15]
ATGAAAATAATTAAGGGCTTACGTCAGGTTGTATTCGCGGTGCCGGCAATGTTGTTGCTGATGTCTGCTGATGCCTTGGC
TGTCACACTGAAAGACATAAAATTTTCAACCATGGCGGGTGATATTACCGAGATCGCGTTGGAGTTTGATGGGGCGCCGC
CAGAAGTTAGTGGTTACACCATTGAGAAGCCTGCACGTATCGCAATTGACTTACCAGGTGTGCAGAGTGCTCTACAAACC
AAGCGTCATGAAATTGGTAACGGTAATGCGCGCAGTGCGACCATTGTGGCGACTAAAGATCGTGCACGTCTCGTAGTGAA
TTTAACTCAGCTGGCGGCTTACACAACAACAGCCAACGGTAATACCTTACTTGTTCGAATCGGAGAGCGTTCGGAAGAGA
AAGTATTCGCCGGAAACACAGTTACCAGTGCTTCAGGCGCATCAGTTAGTCCTGCTAGCGATATGAAAGTCGTTAACGTT
GATTTCCGTCGTGGAGAGCTAGGTGAGGGTCAAATTCAGATCATGCTGAACGATGCGCGAGCGTCTGCCAACATACGTCG
TGAAGGTTCAAAAATTATTGCAGATTTAAGTGGCGTGCGTTTACCTGATGTATTAAGCCGTCGACTTGATGTCACTGATT
TCGCCACGCCCGTTAAATATGTTGATGCGCGTCGTGAAGATGGCGTAACACGTATTGTGATTGAGCCGACTTCGGATAAC
TTTGAGTATTTGGCCTATCAAACCGATAAATTGTTATCGATCAACGTATCTGAGCCACCAAAAGATCAAAAAGAAGCACA
GAAGAAGCAATTTCCATTTACCGGTGAAAAACTATCTCTTAACTTCCAAAATATCGAAGTTCGCGCTGTGTTGCAGTTGA
TTGCGGACTTCACTGGTTTGAACTTAGTTGCCTCAGATTCGGTGCAGGGGGCGATTACGCTGCGTTTGCAGAATGTGCCT
TGGGATCAAGCATTGGATTTAGTGCTAAAAACCAAAGGTTTAGGGCAGCGTCAAATGGGCTCTGTGCTCTTGATTGCACC
AGCGGCTGAAATTGCTGCGCGTGAAAAAGTGGAGCTCGAAGCGGTTCGTCAAGTTGAAGAACTGGCACCGTTAGTTACGG
AATACATGCAGCTTAAATATGCCAAAGCATCTGTACTGGCTACATTGTTGACGTCTGAGCAAGGTTTGTTATCAGAGCGC
GGTACTGCGGTAGTTGATGAACGTACCAATACTTTGTTAATGAAAGACACTTCTGCAAACTTAGAAAAAGTACGCGAAGC
TATCATTATGCTGGACGTTCCTGTTCGCCAAGTGCTGATTGAGGCTCGCATTGTGGTAGCAAACACCAGTGTTGGTAAAG
AACTCGGCGTGAAATGGGGTGGGGCTTCGTTTAAAGACAATGGCAGCAATTGGACCACTGCTGGTGGCAGTCAGCAAACC
TTAACAGAAGGCTCGCAGATCTTGTTTGACCGCGCGACCACTGGCAGTTCTACACAAAATATCGATTTGACCAACGCCAA
CGTAGTGGATTTCGGTGCTACAAACGCAGCAGCGTCAACGTTCGCGATTGGATATCAAACAGCAGACTTCTTGTTGGATC
TAGAGTTGTCGGCGATTGAAACCGAAGGTCGTGCTGAAATTGTATCCCAGCCGCGTGTCATTACGGCGGATGGTCAGACT
GCCTCTATTGAGTCAGGTACAGAAATTCCATACCAGCAAGCTAGCTCAAGTGGCGCGACTAACGTGGCCTTTAAGTCAGC
TGTTTTACGCTTGGAAGTGACTCCGCAAATTACTCCTGATGATCGAATTATCATGGATTTGCTCATTAATCAGGACTCTG
TTGGCGAGCAAACCTCAGCTGGTCCTAGTATCAATACCAATGCGGTTGAGACTCAAGTGTTAGTTGAGAATGGCGAAACA
GTAGTACTTGGTGGTATCTTCCGTTCTGAAGATGTGACCACCATCCGTAAAACACCGTTTTTTGGCGATTTACCTCTGAT
TGGTGCGTTGTTCCGCTATAAGAGTAAGACGGATACTAAGAGTGAGCTGCTTGTGTTCATTACCCCTCGTTTGGTGAAGG
ATACGCTCAGCACTCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-437)

Bacterial type II and III secretion system protein (Secretin)

(538-693)

AMIN domain (AMIN)

(41-127)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.515

100

0.491
  pilQ Acinetobacter baumannii strain A118

41.918

100

0.438
  pilQ Acinetobacter baumannii D1279779

41.918

100

0.438
  comQ Acinetobacter baylyi ADP1

40.251

100

0.413
  pilQ Legionella pneumophila strain Lp02

39.97

94.134

0.376
  pilQ Legionella pneumophila strain ERS1305867

40.031

93.276

0.373
  pilQ Neisseria gonorrhoeae strain FA1090

34.497

100

0.368

Multiple sequence alignment