Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R615_RS14960 Genome accession   NZ_CP006829
Coordinates   3290952..3292049 (-) Length   365 a.a.
NCBI ID   WP_015488346.1    Uniprot ID   -
Organism   Thalassolituus oleivorans R6-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3285952..3297049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R615_RS14935 (R615_15240) aroK 3286592..3287110 (-) 519 WP_197019469.1 shikimate kinase AroK -
  R615_RS14940 (R615_15245) pilQ 3287115..3289214 (-) 2100 WP_025266364.1 type IV pilus secretin PilQ Machinery gene
  R615_RS14945 (R615_15250) - 3289217..3289756 (-) 540 WP_025266365.1 pilus assembly protein PilP -
  R615_RS14950 (R615_15255) - 3289758..3290396 (-) 639 WP_025266366.1 type 4a pilus biogenesis protein PilO -
  R615_RS14955 (R615_15260) - 3290398..3290952 (-) 555 WP_015488345.1 PilN domain-containing protein -
  R615_RS14960 (R615_15265) pilM 3290952..3292049 (-) 1098 WP_015488346.1 pilus assembly protein PilM Machinery gene
  R615_RS14965 (R615_15270) - 3292182..3294776 (+) 2595 WP_025266368.1 penicillin-binding protein 1A -
  R615_RS14970 (R615_15275) - 3294838..3296241 (-) 1404 WP_197019470.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 39515.55 Da        Isoelectric Point: 4.2678

>NTDB_id=114317 R615_RS14960 WP_015488346.1 3290952..3292049(-) (pilM) [Thalassolituus oleivorans R6-15]
MQAREFDGDFVVLASLFNKKNKALLGVDISSTSVKVLELSRQGDRYQVEAYATEPLPLNAVVEQSISNDEAVGEAIRKAI
ARSRTSAKAAAIAVAGSAVITKTVHMSATLKDDEMDFQIRAEADQYIPYPLEEVALDWEIQGPSEGLADMVDVLLAACRS
ETVDRRKDAVEYAGISVERVDVEAFCTERAFALLGAQVVGEDVETVAVLDIGATMTTLSVLHEGKSIYTREQLFGGKQLT
EDIKRRYGLSDEEAQRAKIEGGVPDDYESEVLDPFRQSVVQQVSRSLQFFYSSSQFNDVDCIVLAGGTSAIAGLADRVQE
TLGIPTVVANPFVSMTLSQKVNANLLNSDAPSLMIAAGLALRSFD

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=114317 R615_RS14960 WP_015488346.1 3290952..3292049(-) (pilM) [Thalassolituus oleivorans R6-15]
ATGCAAGCTCGCGAATTTGATGGGGATTTTGTCGTGCTGGCTAGCCTGTTTAACAAGAAAAATAAGGCGCTTCTGGGCGT
GGACATAAGCTCAACGTCAGTCAAAGTGCTCGAGCTCAGCCGTCAAGGCGATCGCTATCAGGTGGAAGCCTATGCGACAG
AGCCTCTCCCGCTCAATGCGGTTGTAGAGCAGAGTATTAGCAATGACGAAGCGGTAGGTGAAGCAATTCGTAAAGCAATT
GCTCGTTCGCGCACGAGTGCAAAAGCAGCAGCCATTGCGGTTGCTGGCTCAGCGGTTATTACTAAAACCGTTCATATGAG
TGCGACTCTTAAAGACGATGAGATGGATTTTCAGATTCGCGCAGAAGCGGATCAGTATATTCCATACCCTCTCGAAGAGG
TCGCGCTTGATTGGGAAATTCAAGGCCCATCAGAAGGATTAGCCGATATGGTTGATGTCCTTTTGGCTGCTTGTCGCTCT
GAAACCGTTGATCGTCGTAAAGACGCAGTCGAATATGCCGGTATTTCGGTAGAGCGCGTCGACGTTGAAGCATTTTGCAC
AGAGCGTGCCTTTGCACTCTTGGGAGCGCAGGTTGTTGGTGAAGATGTGGAGACCGTCGCAGTTCTTGATATTGGCGCGA
CCATGACCACGCTTAGTGTTCTTCATGAAGGGAAATCAATTTACACTCGCGAACAGTTGTTCGGGGGGAAGCAATTGACG
GAAGACATTAAAAGACGTTACGGCTTGTCGGATGAAGAAGCACAGCGTGCAAAAATAGAAGGTGGTGTTCCTGATGATTA
TGAATCTGAAGTCTTAGATCCATTTCGCCAGTCGGTTGTGCAGCAAGTGAGTCGTTCATTGCAATTTTTCTATTCATCAA
GTCAATTTAATGATGTCGACTGTATTGTTTTAGCAGGTGGTACGTCAGCGATTGCTGGATTGGCAGATAGAGTTCAAGAA
ACTCTGGGTATCCCTACCGTGGTGGCAAATCCTTTCGTTAGCATGACGCTGTCGCAAAAGGTAAATGCGAATTTGTTAAA
TAGTGATGCACCTTCTCTAATGATTGCGGCAGGATTGGCATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(26-364)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.714

95.89

0.515
  comM Acinetobacter nosocomialis M2

53.714

95.89

0.515
  comM Acinetobacter baylyi ADP1

50.286

95.89

0.482
  pilM Legionella pneumophila strain ERS1305867

43.099

97.26

0.419

Multiple sequence alignment