Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   SHEWBE_RS03870 Genome accession   NZ_LS483452
Coordinates   822822..824093 (-) Length   423 a.a.
NCBI ID   WP_112351594.1    Uniprot ID   A0A330LY92
Organism   Shewanella benthica strain DB21MT-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817822..829093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWBE_RS03840 (SHEWBE_0896) - 818449..819318 (-) 870 WP_112351588.1 LysR substrate-binding domain-containing protein -
  SHEWBE_RS03845 (SHEWBE_0897) mutT 819441..819812 (-) 372 WP_231926407.1 8-oxo-dGTP diphosphatase MutT -
  SHEWBE_RS03850 (SHEWBE_0898) yacG 819872..820084 (-) 213 WP_112351590.1 DNA gyrase inhibitor YacG -
  SHEWBE_RS03855 (SHEWBE_0899) zapD 820190..820924 (-) 735 WP_112351591.1 cell division protein ZapD -
  SHEWBE_RS03860 (SHEWBE_0900) coaE 820933..821538 (-) 606 WP_112351592.1 dephospho-CoA kinase -
  SHEWBE_RS03865 (SHEWBE_0901) pilD 821851..822762 (-) 912 WP_112351593.1 prepilin peptidase Machinery gene
  SHEWBE_RS03870 (SHEWBE_0902) pilC 822822..824093 (-) 1272 WP_112351594.1 type II secretion system F family protein Machinery gene
  SHEWBE_RS03875 (SHEWBE_0903) pilB 824135..825841 (-) 1707 WP_112351595.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SHEWBE_RS20870 (SHEWBE_0904) - 825930..826388 (-) 459 WP_112351596.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SHEWBE_RS03885 (SHEWBE_0905) nadC 827137..828054 (-) 918 WP_112351597.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SHEWBE_RS03890 (SHEWBE_0907) ampD 828274..828861 (+) 588 WP_231926551.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45837.08 Da        Isoelectric Point: 10.0841

>NTDB_id=1142115 SHEWBE_RS03870 WP_112351594.1 822822..824093(-) (pilC) [Shewanella benthica strain DB21MT-2]
MASASIIKKRQARAKRTNANSQAKIYTFEWKGVNKAGQKTSGELKGATAAEVRSQLKTQGVSPKSVKKQSASLFQLGAPK
ITAMDIAMITRQIATMLSAGVPLVTTIEMLGRGHEKAKMRELLGTIVNDVQAGIPLSDALRPHRQYFDDLYVDLVAAGEH
SGSLDAVFDRVATYREKAEALKSKIKKAMFYPAAVVVVAIGVTSLLLLFVVPQFQEIFAGFGAELPAFTQFVIGISEALQ
ASWYIFAVIIMVSIFLFKRAHRNSQLFRDKVDVLLLKIPIIGPILHKAAMARFARTLATTFAAGVPLIDGLESSAGASGN
AVYRKAVLKVRTEVMAGMQMNVAMSTTNLFPDMLIQMVMIGEESGGLDDMLNKVANIYEMQVDDAVDGLSSLIEPIMMVV
IGTLVGGLIVAMYLPIFEMGKII

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=1142115 SHEWBE_RS03870 WP_112351594.1 822822..824093(-) (pilC) [Shewanella benthica strain DB21MT-2]
ATGGCTAGCGCAAGCATCATTAAAAAAAGACAGGCAAGAGCTAAGAGAACCAATGCCAACAGCCAAGCTAAGATCTATAC
CTTCGAATGGAAAGGAGTCAACAAAGCCGGTCAAAAAACCTCGGGTGAGCTTAAAGGTGCCACGGCTGCCGAAGTGAGAA
GTCAGCTAAAGACTCAGGGGGTCAGCCCCAAATCGGTGAAGAAGCAATCGGCGAGTCTCTTCCAGCTGGGTGCTCCCAAG
ATCACCGCCATGGATATCGCCATGATCACCCGCCAAATAGCCACCATGCTCTCGGCCGGGGTGCCACTGGTGACCACCAT
AGAGATGCTGGGCCGCGGCCATGAGAAAGCCAAGATGCGCGAGCTGCTTGGCACGATCGTAAACGATGTCCAAGCCGGTA
TTCCGCTCTCTGATGCATTAAGGCCCCATAGGCAATATTTCGATGATCTCTATGTGGATCTGGTCGCCGCGGGGGAACAC
TCGGGATCCTTAGATGCCGTGTTCGATCGCGTCGCAACCTACAGGGAGAAAGCCGAGGCACTTAAATCTAAAATCAAGAA
GGCCATGTTCTACCCGGCTGCCGTGGTGGTGGTTGCCATAGGCGTGACATCATTACTGTTACTGTTTGTGGTGCCACAGT
TTCAGGAAATTTTTGCCGGTTTCGGTGCCGAACTGCCTGCCTTTACTCAGTTCGTCATCGGAATATCGGAGGCACTGCAA
GCCTCCTGGTATATATTTGCGGTGATCATCATGGTCAGCATTTTTCTATTTAAGCGTGCCCATAGAAATTCGCAATTGTT
TAGAGATAAAGTCGATGTTTTACTCCTTAAAATACCGATTATTGGCCCCATACTGCATAAGGCCGCCATGGCGCGGTTCG
CACGTACACTGGCGACCACATTTGCTGCAGGCGTCCCGCTAATCGATGGCTTAGAGTCCTCCGCTGGCGCATCGGGCAAT
GCCGTTTATCGTAAAGCGGTACTCAAGGTCCGCACCGAAGTGATGGCGGGCATGCAGATGAACGTTGCCATGAGTACCAC
TAACCTGTTTCCCGATATGCTGATCCAGATGGTGATGATAGGTGAAGAGTCCGGTGGACTCGATGATATGCTCAACAAGG
TCGCTAATATTTACGAGATGCAGGTCGATGACGCCGTGGATGGCCTCTCTAGCCTGATCGAACCCATCATGATGGTGGTG
ATTGGTACGCTAGTCGGGGGCTTAATCGTCGCCATGTATCTTCCCATCTTCGAAATGGGTAAGATTATCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(293-415)

Type II secretion system (T2SS), protein F (T2SSF)

(90-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A330LY92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.808

93.617

0.522
  pilC Acinetobacter baumannii D1279779

51.005

94.09

0.48
  pilC Legionella pneumophila strain ERS1305867

50.758

93.617

0.475
  pilC Acinetobacter baylyi ADP1

50.63

93.853

0.475
  pilC Vibrio cholerae strain A1552

48.866

93.853

0.459
  pilC Vibrio campbellii strain DS40M4

46.135

94.799

0.437
  pilG Neisseria meningitidis 44/76-A

42.317

93.853

0.397
  pilG Neisseria gonorrhoeae MS11

42.317

93.853

0.397

Multiple sequence alignment