Detailed information NTG1142114

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: SHEWBE_RS03865
• Genome accession: NZ_LS483452
• Coordinates: 821851..822762 (-)
• Length: 303 a.a.
• NCBI ID: WP_112351593.1
• Uniprot ID: A0A330M127
• Organism: Shewanella benthica strain DB21MT-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 816851..827762

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SHEWBE_RS03840 (SHEWBE_0896)	-	818449..819318 (-)	870	WP_112351588.1	LysR substrate-binding domain-containing protein	-
SHEWBE_RS03845 (SHEWBE_0897)	mutT	819441..819812 (-)	372	WP_231926407.1	8-oxo-dGTP diphosphatase MutT	-
SHEWBE_RS03850 (SHEWBE_0898)	yacG	819872..820084 (-)	213	WP_112351590.1	DNA gyrase inhibitor YacG	-
SHEWBE_RS03855 (SHEWBE_0899)	zapD	820190..820924 (-)	735	WP_112351591.1	cell division protein ZapD	-
SHEWBE_RS03860 (SHEWBE_0900)	coaE	820933..821538 (-)	606	WP_112351592.1	dephospho-CoA kinase	-
SHEWBE_RS03865 (SHEWBE_0901)	pilD	821851..822762 (-)	912	WP_112351593.1	prepilin peptidase	Machinery gene
SHEWBE_RS03870 (SHEWBE_0902)	pilC	822822..824093 (-)	1272	WP_112351594.1	type II secretion system F family protein	Machinery gene
SHEWBE_RS03875 (SHEWBE_0903)	pilB	824135..825841 (-)	1707	WP_112351595.1	type IV-A pilus assembly ATPase PilB	Machinery gene
SHEWBE_RS20870 (SHEWBE_0904)	-	825930..826388 (-)	459	WP_112351596.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 303 a.a.
• Molecular weight: 33516.82 Da
• Isoelectric Point: 7.0083

>NTDB_id=1142114 SHEWBE_RS03865 WP_112351593.1 821851..822762(-) (pilD) [Shewanella benthica strain DB21MT-2]
MSELITILSHNLWLFSAIAFIFAAVIGSFLNVVIHRLPVMMKREWQQECNQYLNEYHQELIAPISKKLERAIDGYPEKYN
LIVPGSACPKCKTDIKPWHNLPVIGWLMLRGKCASCSTAISARYPVIELLTGLAVAFLALHFGPTWEFACATLLTLVLIA
LTGIDLDEMLLPDQLTLPLLWFGLLINLNGTFASSSDALIGAAAGYLSLWSVFWAFKLLTGKDGMGYGDFKLLAVFGAWF
GWQLLPLIILLSSVVGAVIGIMLIISKKLNHGNPIPFGPYIALAGWIAMIWGSDINTWYLSTL

Nucleotide

• Length: 912 bp

>NTDB_id=1142114 SHEWBE_RS03865 WP_112351593.1 821851..822762(-) (pilD) [Shewanella benthica strain DB21MT-2]
ATGTCAGAATTAATCACCATATTAAGCCACAACTTGTGGCTCTTTAGCGCCATCGCTTTTATCTTTGCCGCCGTCATAGG
TAGCTTTCTCAATGTGGTCATTCATCGCTTGCCGGTGATGATGAAACGTGAGTGGCAGCAAGAATGTAACCAATACCTCA
ACGAGTATCATCAAGAGCTTATAGCGCCCATCTCAAAAAAACTCGAACGAGCCATCGATGGTTATCCTGAGAAATATAAC
CTGATCGTGCCGGGTTCGGCCTGCCCGAAATGCAAGACCGATATTAAGCCCTGGCATAACCTGCCTGTTATTGGCTGGCT
GATGCTAAGAGGAAAGTGCGCCTCGTGCAGCACAGCCATATCGGCGCGCTACCCAGTCATAGAATTATTGACAGGCTTGG
CGGTGGCTTTTCTCGCGCTGCATTTCGGCCCGACCTGGGAATTTGCCTGCGCAACCTTACTCACCTTGGTGCTGATAGCC
CTAACAGGTATAGACCTCGATGAGATGCTGCTTCCCGACCAACTCACCCTCCCCCTGCTATGGTTCGGTTTATTGATTAA
CCTAAATGGCACCTTTGCCAGCAGCAGCGACGCATTAATCGGCGCCGCCGCGGGTTATCTGAGCCTCTGGAGTGTCTTCT
GGGCATTTAAGCTATTAACCGGCAAGGATGGCATGGGCTATGGTGATTTTAAGCTGTTAGCCGTGTTTGGTGCCTGGTTT
GGTTGGCAACTACTGCCACTCATCATACTGCTATCATCGGTAGTCGGCGCAGTCATTGGCATCATGCTGATCATCAGTAA
AAAACTCAATCACGGTAATCCAATTCCCTTTGGCCCTTATATCGCGCTAGCGGGCTGGATAGCCATGATCTGGGGCAGTG
ATATCAATACCTGGTATCTATCCACCCTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-142)

• Type IV leader peptidase family (Peptidase_A24) (153-261)

3D structure

• AlphaFold DB: A0A330M127

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.082	96.37	0.512
pilD	Vibrio campbellii strain DS40M4	50.667	99.01	0.502
pilD	Acinetobacter baumannii D1279779	48.837	99.34	0.485
pilD	Acinetobacter nosocomialis M2	48.837	99.34	0.485
pilD	Neisseria gonorrhoeae MS11	45.556	89.109	0.406