Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DQO04_RS02115 Genome accession   NZ_LS483433
Coordinates   469151..470491 (+) Length   446 a.a.
NCBI ID   WP_084379833.1    Uniprot ID   -
Organism   Pseudomonas mucidolens strain NCTC8068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 464151..475491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQO04_RS02095 (NCTC8068_00415) pilM 466739..467392 (+) 654 WP_084379837.1 type IV pilus biogenesis protein PilM -
  DQO04_RS02100 (NCTC8068_00416) - 467392..467943 (+) 552 WP_084379836.1 PilN domain-containing protein -
  DQO04_RS02105 (NCTC8068_00417) - 467940..468560 (+) 621 WP_084379835.1 type 4a pilus biogenesis protein PilO -
  DQO04_RS02110 (NCTC8068_00418) - 468557..469081 (+) 525 WP_084379834.1 pilus assembly protein PilP -
  DQO04_RS02115 (NCTC8068_00419) pilQ 469151..470491 (+) 1341 WP_084379833.1 type IV pilus secretin PilQ Machinery gene
  DQO04_RS02120 (NCTC8068_00420) aroK 470496..471014 (+) 519 WP_084379832.1 shikimate kinase AroK -
  DQO04_RS02125 (NCTC8068_00421) aroB 471162..472262 (+) 1101 WP_084379831.1 3-dehydroquinate synthase -
  DQO04_RS02130 (NCTC8068_00422) - 472273..473823 (+) 1551 WP_084379830.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 48286.86 Da        Isoelectric Point: 4.7057

>NTDB_id=1141359 DQO04_RS02115 WP_084379833.1 469151..470491(+) (pilQ) [Pseudomonas mucidolens strain NCTC8068]
MVIAVPNRVELIQLPPPGAASLSNGRGAFTGDKLSLNFQNIEVRAVLQQIADVAGLNLVAADDVGGSITLRLKDVPWDQA
LDLVLQAKGLDKRVSANVLLVASAQELAERELRALESRRQLTELAPLRRELLQVNYANAAQLAKLFQSVSGLEGASDERG
SVAVDDRTNNIIVYQTQERLDELRHIVAQLDIPIRQVMIEARIVEANVDYDKSTGVRWGGLIQNKGNWSGGGINNGSSEE
AGQSESTPSSPFVDLGVVNGSSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSS
GATSVSFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSKTQSNVV
DKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=1141359 DQO04_RS02115 WP_084379833.1 469151..470491(+) (pilQ) [Pseudomonas mucidolens strain NCTC8068]
ATGGTCATCGCCGTGCCCAATCGGGTCGAGCTGATTCAACTTCCACCGCCTGGTGCTGCGTCGCTGAGTAACGGACGTGG
CGCGTTTACCGGCGATAAATTATCCCTCAACTTCCAGAACATCGAGGTCCGCGCTGTCTTGCAGCAGATCGCCGACGTTG
CCGGCCTCAATCTGGTGGCCGCTGATGATGTCGGCGGCTCGATTACCTTGCGTCTCAAAGACGTACCGTGGGACCAGGCG
CTGGATCTGGTGCTGCAAGCCAAGGGGTTGGACAAGCGGGTGAGCGCTAACGTGCTGTTGGTGGCATCGGCGCAGGAATT
GGCCGAGCGTGAGTTGCGGGCTCTGGAGTCGCGCAGGCAGTTGACTGAACTGGCACCGCTGCGGCGCGAGCTGCTGCAAG
TCAACTATGCCAATGCCGCGCAACTGGCCAAGTTGTTCCAGTCGGTGAGCGGCCTGGAAGGCGCGTCCGATGAACGTGGT
TCGGTGGCGGTGGATGATCGGACCAACAACATCATCGTCTACCAAACCCAGGAGCGCCTGGACGAGTTGCGGCATATCGT
TGCGCAACTGGATATTCCGATTCGCCAGGTCATGATCGAGGCGCGGATCGTCGAAGCCAATGTCGATTACGACAAGAGTA
CGGGCGTGCGCTGGGGAGGGCTGATACAGAATAAGGGCAATTGGAGCGGTGGCGGCATCAATAACGGATCTTCAGAGGAG
GCCGGGCAGTCGGAGAGTACGCCCAGTTCACCGTTTGTCGACTTGGGCGTGGTCAATGGCTCGTCCGGCCTCGGTATCGC
TTTTATCACCGATAACGTGCTGCTCGATCTGGAGCTGACGGCGATGGAAAAAACCGGTAACGGTGAAATCGTCTCGCAGC
CCAAAGTGGTCACGTCCGACAAGGAAACCGCGCGGATCCTCAAGGGCACCGAGATTCCCTACCAGGAGTCCAGTTCCAGC
GGGGCTACCTCGGTGTCGTTCAAGGAGGCTTCGTTATCCCTGGAGGTGACGCCGCAAATCACCCCGGACGACCGGGTAAT
CATGGAGGTGAAAGTCACCAAGGATGAGCCTGACTACCTGAATAAACTCAACGACGTGCCGCCCATCAAGAAAAACGAGG
TCAATGCCAAGGTGCTGGTCAAGGATGGCGAGACCATTGTCATTGGCGGCGTTTTCTCGAAAACTCAAAGCAACGTGGTA
GATAAAGTGCCATTTTTGGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGGGATGTGGTCTCGGAGAAAAAATCCGA
GCTGCTGGTATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(58-102)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)

Bacterial type II and III secretion system protein (Secretin)

(281-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.548

98.206

0.693
  comQ Acinetobacter baylyi ADP1

43.326

100

0.444
  pilQ Acinetobacter baumannii D1279779

41.949

100

0.444
  pilQ Acinetobacter baumannii strain A118

41.949

100

0.444
  pilQ Vibrio campbellii strain DS40M4

43.972

94.843

0.417
  pilQ Legionella pneumophila strain ERS1305867

42.588

95.291

0.406
  pilQ Legionella pneumophila strain Lp02

42.588

95.291

0.406
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.301

93.722

0.406
  pilQ Vibrio cholerae strain A1552

43.301

93.722

0.406

Multiple sequence alignment