Detailed information NTG1140557

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DQN21_RS03980
• Genome accession: NZ_LS483409
• Coordinates: 728841..729506 (+)
• Length: 221 a.a.
• NCBI ID: WP_077496500.1
• Uniprot ID: -
• Organism: Streptococcus gallolyticus strain NCTC13773
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 723841..734506

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN21_RS03960 (NCTC13773_00797)	-	725260..726840 (+)	1581	WP_077496496.1	DEAD/DEAH box helicase	-
DQN21_RS03965 (NCTC13773_00798)	-	726884..727156 (-)	273	WP_009853764.1	GIY-YIG nuclease family protein	-
DQN21_RS03970 (NCTC13773_00799)	-	727131..727892 (-)	762	WP_077496498.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
DQN21_RS03975 (NCTC13773_00800)	-	728000..728746 (+)	747	WP_009853766.1	lysophospholipid acyltransferase family protein	-
DQN21_RS03980 (NCTC13773_00801)	comEA	728841..729506 (+)	666	WP_077496500.1	helix-hairpin-helix domain-containing protein	Machinery gene
DQN21_RS03985 (NCTC13773_00802)	comEC/celB	729499..731730 (+)	2232	WP_077496502.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DQN21_RS03990 (NCTC13773_00803)	holA	731804..732841 (+)	1038	WP_077498002.1	DNA polymerase III subunit delta	-
DQN21_RS03995 (NCTC13773_00804)	sodA	732930..733538 (+)	609	WP_061458979.1	superoxide dismutase SodA	-
DQN21_RS04000 (NCTC13773_00805)	-	733690..734415 (+)	726	WP_077496504.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23403.06 Da
• Isoelectric Point: 4.3235

>NTDB_id=1140557 DQN21_RS03980 WP_077496500.1 728841..729506(+) (comEA) [Streptococcus gallolyticus strain NCTC13773]
MIEEIKEKLLENKTLVAVLGTIIVMLIGFFAWSNVTKTTADAQSDLPALSTTFSASSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSSGSRVTDVVKLAGGFTEDADKKSVNLAEKVTDEAVIYVARVGENVAPATTNSQANGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=1140557 DQN21_RS03980 WP_077496500.1 728841..729506(+) (comEA) [Streptococcus gallolyticus strain NCTC13773]
ATGATTGAAGAAATAAAAGAAAAACTTTTGGAGAACAAAACGTTGGTAGCGGTGTTGGGAACAATAATTGTCATGTTAAT
CGGATTTTTTGCTTGGTCAAATGTGACAAAAACAACAGCGGATGCGCAAAGTGACCTACCTGCCCTAAGCACGACTTTTT
CGGCAAGTAGCAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAAACTCAAAAAGTGTTTGTAGATATC
AAAGGCGCTGTGAAAAATGAGGGGGTTTATGAGCTTTCAAGTGGCAGTCGTGTGACAGATGTGGTCAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATTTGGCTGAAAAAGTAACCGATGAAGCGGTGATTTATGTGGCAAGAG
TTGGTGAAAATGTTGCTCCTGCAACGACTAATTCACAAGCAAATGGCAGCGCTCAACAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCGGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGACATTATTGACTA
CCGTGATGCCAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGTATCGGAGAAAAAACACTAGAAAAAC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (157-219)

• SLBB domain (SLBB) (78-131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	48.069	100	0.507
comEA/celA/cilE	Streptococcus mitis SK321	47.059	100	0.471
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	45.74	100	0.462
comEA/celA/cilE	Streptococcus pneumoniae Rx1	44.843	100	0.452
comEA/celA/cilE	Streptococcus pneumoniae D39	44.843	100	0.452
comEA/celA/cilE	Streptococcus pneumoniae R6	44.843	100	0.452
comEA/celA/cilE	Streptococcus mitis NCTC 12261	44.395	100	0.448
comEA	Latilactobacillus sakei subsp. sakei 23K	37.668	100	0.38
comEA	Staphylococcus aureus MW2	36.036	100	0.362