Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN96_RS05075 Genome accession   NZ_LS483406
Coordinates   1086000..1087094 (+) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas maltophilia strain NCTC10498     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1081000..1092094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN96_RS05050 (NCTC10498_01013) - 1081456..1082058 (-) 603 WP_049397188.1 short chain dehydrogenase -
  DQN96_RS05055 (NCTC10498_01014) - 1082157..1083032 (+) 876 WP_060381038.1 LysR family transcriptional regulator -
  DQN96_RS05060 (NCTC10498_01015) proC 1083209..1084030 (-) 822 WP_060381039.1 pyrroline-5-carboxylate reductase -
  DQN96_RS05065 (NCTC10498_01016) - 1084049..1084726 (-) 678 WP_049397192.1 YggS family pyridoxal phosphate-dependent enzyme -
  DQN96_RS05070 (NCTC10498_01017) pilT 1084814..1085851 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  DQN96_RS05075 (NCTC10498_01018) pilU 1086000..1087094 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN96_RS05080 (NCTC10498_01019) - 1087302..1088285 (+) 984 WP_111722884.1 aldo/keto reductase -
  DQN96_RS05085 (NCTC10498_01020) - 1088333..1089232 (-) 900 WP_005412489.1 LysR family transcriptional regulator -
  DQN96_RS05090 (NCTC10498_01021) - 1089326..1089535 (+) 210 WP_005412490.1 DUF4287 domain-containing protein -
  DQN96_RS05095 (NCTC10498_01022) - 1089646..1090353 (+) 708 WP_014036228.1 YitT family protein -
  DQN96_RS05100 (NCTC10498_01023) - 1090437..1090982 (-) 546 WP_005408375.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=1140412 DQN96_RS05075 WP_043033789.1 1086000..1087094(+) (pilU) [Stenotrophomonas maltophilia strain NCTC10498]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=1140412 DQN96_RS05075 WP_043033789.1 1086000..1087094(+) (pilU) [Stenotrophomonas maltophilia strain NCTC10498]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACTCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCATGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGCACCGGTAAATCGACGTCGCTGGCAGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATTGGTGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAATTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGCTGTCACAGGGCCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363

Multiple sequence alignment