Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MORIYA_RS15630 Genome accession   NZ_LS483250
Coordinates   3363096..3364799 (-) Length   567 a.a.
NCBI ID   WP_112716616.1    Uniprot ID   -
Organism   Moritella yayanosii strain DB21MT 5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3358096..3369799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MORIYA_RS21840 - 3359273..3359359 (+) 87 Protein_3008 SEC-C metal-binding domain-containing protein -
  MORIYA_RS15605 (MORIYA_3290) mutT 3359508..3359900 (+) 393 WP_112716606.1 8-oxo-dGTP diphosphatase MutT -
  MORIYA_RS15610 (MORIYA_3291) yacG 3359932..3360123 (-) 192 WP_232011740.1 DNA gyrase inhibitor YacG -
  MORIYA_RS15615 (MORIYA_3292) coaE 3360152..3360757 (-) 606 WP_112716610.1 dephospho-CoA kinase -
  MORIYA_RS15620 (MORIYA_3293) pilD 3360788..3361675 (-) 888 WP_112716612.1 A24 family peptidase Machinery gene
  MORIYA_RS15625 (MORIYA_3294) pilC 3361763..3363019 (-) 1257 WP_112716614.1 type II secretion system F family protein Machinery gene
  MORIYA_RS15630 (MORIYA_3295) pilB 3363096..3364799 (-) 1704 WP_112716616.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MORIYA_RS15635 (MORIYA_3296) - 3364806..3365246 (-) 441 WP_112716618.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MORIYA_RS15640 (MORIYA_3297) nadC 3365637..3366479 (-) 843 WP_112716620.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MORIYA_RS15645 (MORIYA_3298) - 3366504..3367010 (-) 507 WP_112716622.1 TIGR02281 family clan AA aspartic protease -
  MORIYA_RS15650 (MORIYA_3299) ampD 3367221..3367784 (+) 564 WP_112716624.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MORIYA_RS15655 (MORIYA_3300) ampE 3367786..3368634 (+) 849 WP_112716626.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62755.84 Da        Isoelectric Point: 4.9781

>NTDB_id=1135270 MORIYA_RS15630 WP_112716616.1 3363096..3364799(-) (pilB) [Moritella yayanosii strain DB21MT 5]
MPHQHNASGLAHSLVAHSYLDVEEIPQLLQSAKQEGKAFTTYIVDNSIIDSQILAQLLEREYGVPLLDLDTFNLEEIPDN
LLNEKLIEKHHALPIYMQGQTLYLAMSDPTNVSALEEFAFSFSLHTEVLLVDELQLQKALDTVLESDIDALGDLNDADIG
DLEVDNSESRLEQESKDGADDAPIVKFVNKILLDAIKKGASDIHFEPYEFKYRVRFRIDGILHEMVSPPVNLAMRFSARL
KVMAKLDIAERRVPQDGRIKLKLSKNKSIDLRVSTLPTMWGEKVVMRILDSNQASLNIDILGYDEKQKALYLKALTKPQG
MILITGPTGSGKTVSLYSGLNILNTVERNISTAEDPIEINLSGINQVQINLKAGLTFPVALRSFLRQDPDVVMVGEIRDI
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIGSSVSLIIAQRLGRRLCNECKQQEDIPASELAKFGFSQ
PQIDHGITPFKAVGCNQCTKGYKGRVGIYEVMPMSDTISRMILTGSNSMALHDQAQQEGMDTLRQSALNKVIDGITSLLE
VERITSH

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1135270 MORIYA_RS15630 WP_112716616.1 3363096..3364799(-) (pilB) [Moritella yayanosii strain DB21MT 5]
ATGCCACACCAGCATAACGCGAGCGGCCTGGCACATAGCCTGGTCGCACATTCATACCTCGATGTAGAAGAGATCCCACA
ACTTCTGCAAAGCGCCAAACAGGAAGGTAAAGCCTTCACGACTTATATCGTCGACAATAGTATCATCGACAGTCAAATAT
TAGCGCAATTATTAGAACGTGAATACGGCGTGCCACTGCTCGACTTAGATACCTTTAATCTCGAAGAAATCCCCGATAAT
CTGCTCAATGAAAAACTCATTGAGAAACATCATGCCTTACCTATTTATATGCAGGGCCAAACTCTATACCTAGCGATGTC
AGATCCAACAAATGTAAGCGCACTAGAAGAGTTTGCCTTTAGCTTTAGTTTACATACCGAAGTTCTATTGGTTGATGAAC
TACAATTACAAAAAGCCTTAGACACTGTATTAGAAAGTGATATTGATGCCCTAGGCGATCTTAATGATGCCGATATCGGT
GACTTGGAAGTGGATAATAGCGAGTCACGTTTAGAGCAAGAAAGTAAAGACGGCGCGGATGACGCGCCAATCGTTAAATT
TGTGAATAAAATTCTACTCGATGCGATTAAAAAAGGCGCCTCTGATATTCATTTCGAACCCTATGAGTTTAAATATCGCG
TACGCTTTCGTATTGACGGGATCTTACATGAAATGGTATCGCCACCGGTTAACTTAGCCATGCGCTTTTCCGCGCGTTTG
AAAGTCATGGCCAAATTAGATATTGCCGAGCGCAGAGTGCCACAAGATGGGCGGATAAAATTAAAATTATCCAAGAATAA
ATCCATTGATCTACGTGTCAGCACCCTACCCACCATGTGGGGTGAAAAAGTAGTGATGCGGATCTTAGATTCCAACCAAG
CCAGTCTCAATATCGATATACTCGGTTATGACGAAAAACAAAAAGCGCTCTATCTTAAAGCACTCACCAAACCGCAAGGC
ATGATCCTGATCACCGGACCAACCGGTAGTGGTAAAACCGTGTCTTTGTACAGCGGATTAAATATTCTGAATACCGTGGA
ACGTAACATCTCCACCGCCGAGGATCCTATCGAGATCAATCTCAGCGGCATTAATCAGGTGCAGATCAACCTCAAAGCAG
GACTTACTTTTCCCGTCGCATTACGTTCATTCTTACGTCAAGATCCAGATGTGGTAATGGTCGGTGAGATCCGCGATATA
GAAACAGCAGAGATTGCCATCAAAGCCGCGCAAACCGGTCATTTAGTGTTATCGACATTGCATACCAACTCCGCCGCAGA
AACTCTGACCCGTTTATCAAATATGGGGGTGCCGGCTTATAATATTGGTTCATCCGTCAGCTTGATCATCGCTCAGCGCC
TTGGTCGTCGGCTTTGTAATGAGTGTAAACAACAGGAAGATATTCCGGCAAGTGAATTAGCTAAATTTGGCTTTAGTCAA
CCACAAATTGATCACGGCATTACCCCGTTTAAAGCCGTCGGTTGTAACCAGTGCACCAAGGGTTATAAAGGTCGCGTGGG
GATCTACGAAGTCATGCCCATGTCTGATACGATCTCACGGATGATCTTAACCGGCAGTAACTCCATGGCACTCCACGATC
AAGCTCAGCAGGAAGGCATGGATACCTTACGCCAATCCGCATTAAACAAAGTCATAGACGGGATCACCAGTTTGCTTGAA
GTTGAACGTATCACCAGTCATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-144)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.603

100

0.538
  pilB Acinetobacter baylyi ADP1

52.128

99.471

0.519
  pilB Acinetobacter baumannii D1279779

52.406

98.942

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

52.285

96.473

0.504
  pilB Vibrio cholerae strain A1552

52.908

94.004

0.497
  pilB Vibrio campbellii strain DS40M4

51.201

95.414

0.489
  pilF Neisseria gonorrhoeae MS11

47.5

98.765

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

37.684

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.448

100

0.384
  pilF Thermus thermophilus HB27

37.168

99.647

0.37

Multiple sequence alignment