Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   XTG_RS14125 Genome accession   NZ_LR994544
Coordinates   3313878..3315008 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas euroxanthea isolate X. euroxanthea CPBF 424     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3308878..3320008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XTG_RS14095 (XTG_002819) ruvX 3309292..3309759 (-) 468 WP_104583092.1 Holliday junction resolvase RuvX -
  XTG_RS14100 (XTG_002820) - 3309752..3310318 (-) 567 WP_115677633.1 YqgE/AlgH family protein -
  XTG_RS14105 (XTG_002821) - 3310664..3311203 (+) 540 WP_104648127.1 DNA-3-methyladenine glycosylase I -
  XTG_RS14110 (XTG_002822) - 3311200..3311970 (+) 771 WP_235858472.1 hypothetical protein -
  XTG_RS14115 (XTG_002823) - 3312032..3312931 (+) 900 WP_115677634.1 DUF72 domain-containing protein -
  XTG_RS14120 (XTG_002824) - 3312945..3313619 (-) 675 WP_166763425.1 YitT family protein -
  XTG_RS14125 (XTG_002825) pilU 3313878..3315008 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  XTG_RS14130 (XTG_002826) pilT 3315137..3316174 (-) 1038 WP_104583089.1 type IV pilus twitching motility protein PilT Machinery gene
  XTG_RS14135 (XTG_002827) - 3316499..3317191 (+) 693 WP_115677635.1 YggS family pyridoxal phosphate-dependent enzyme -
  XTG_RS14140 (XTG_002828) proC 3317266..3318120 (+) 855 WP_115677636.1 pyrroline-5-carboxylate reductase -
  XTG_RS14145 (XTG_002829) - 3318723..3319148 (+) 426 WP_104583086.1 HU family DNA-binding protein -
  XTG_RS14150 (XTG_002830) - 3319324..3319902 (+) 579 WP_115677637.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1134990 XTG_RS14125 WP_016902355.1 3313878..3315008(-) (pilU) [Xanthomonas euroxanthea isolate X. euroxanthea CPBF 424]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1134990 XTG_RS14125 WP_016902355.1 3313878..3315008(-) (pilU) [Xanthomonas euroxanthea isolate X. euroxanthea CPBF 424]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACGCCGCTGACCGCGCAACAGAGTCGCGACCTGG
TGCTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTGGAGGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGGATCATCATCTTCGTCGGTG
CCACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGTCGCGGCCGGCGTGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAAGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGACGCCAAGACCCTTGCCCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment