Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLQZX_RS15140 Genome accession   NZ_LR962898
Coordinates   3470895..3472025 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1514     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465895..3477025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLQZX_RS15110 (X12_003006) - 3466238..3466678 (-) 441 WP_016902361.1 OsmC family protein -
  ACLQZX_RS15115 (X12_003007) - 3466931..3467878 (-) 948 WP_039526216.1 aspartate carbamoyltransferase catalytic subunit -
  ACLQZX_RS15120 (X12_003008) ruvX 3467894..3468361 (-) 468 WP_024939388.1 Holliday junction resolvase RuvX -
  ACLQZX_RS15125 (X12_003009) - 3468354..3468920 (-) 567 WP_011051924.1 YqgE/AlgH family protein -
  ACLQZX_RS15130 (X12_003010) - 3469256..3469801 (+) 546 WP_104594471.1 DNA-3-methyladenine glycosylase I -
  ACLQZX_RS15135 (X12_003011) - 3469939..3470613 (-) 675 WP_024940756.1 YitT family protein -
  ACLQZX_RS15140 (X12_003012) pilU 3470895..3472025 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLQZX_RS15145 (X12_003013) pilT 3472148..3473185 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLQZX_RS15150 (X12_003014) - 3473508..3474200 (+) 693 WP_211914097.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLQZX_RS15155 (X12_003015) proC 3474262..3475107 (+) 846 WP_211914098.1 pyrroline-5-carboxylate reductase -
  ACLQZX_RS15160 (X12_003016) - 3475709..3476134 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  ACLQZX_RS15165 (X12_003017) - 3476271..3476891 (+) 621 WP_211914099.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1134965 ACLQZX_RS15140 WP_016902355.1 3470895..3472025(-) (pilU) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1514]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1134965 ACLQZX_RS15140 WP_016902355.1 3470895..3472025(-) (pilU) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1514]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACCCCGCTGACCGCGCAACAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTTGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTGGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGTACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACCCCGGATGGCCGCGGCCGGCGCGTGGCGATGGAGATCATGCTGGGCACACCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCTGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment