Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLVWI_RS16535 Genome accession   NZ_LR962897
Coordinates   3837883..3839592 (-) Length   569 a.a.
NCBI ID   WP_411863116.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3832883..3844592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLVWI_RS16525 (X12_003297) - 3833302..3834915 (+) 1614 WP_411862065.1 sensor histidine kinase -
  ACLVWI_RS16530 (X12_003298) pilR 3835108..3836496 (+) 1389 WP_411862066.1 sigma-54-dependent transcriptional regulator Regulator
  ACLVWI_RS16535 (X12_003299) pilB 3837883..3839592 (-) 1710 WP_411863116.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLVWI_RS16540 (X12_003300) - 3839665..3840177 (-) 513 WP_411862067.1 pilin -
  ACLVWI_RS16545 (X12_003301) pilA/pilAI 3840181..3840609 (-) 429 WP_230433800.1 pilin Machinery gene
  ACLVWI_RS16550 (X12_003302) pilC 3840949..3842205 (+) 1257 WP_411862068.1 type II secretion system F family protein Machinery gene
  ACLVWI_RS16555 (X12_003303) - 3842212..3843075 (+) 864 WP_411862069.1 prepilin peptidase -
  ACLVWI_RS16560 (X12_003304) coaE 3843089..3843694 (+) 606 WP_411862070.1 dephospho-CoA kinase -
  ACLVWI_RS16565 - 3844120..3844566 (+) 447 Protein_3246 DUF6531 domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61600.56 Da        Isoelectric Point: 5.7600

>NTDB_id=1134952 ACLVWI_RS16535 WP_411863116.1 3837883..3839592(-) (pilB) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586]
MGITGIARRLVQDGSLEEGAARSAMDQAAAAKVPLPQWFAEKKLVSAAQLAAANAIEFGMPLMDVSVFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGDDDEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLSQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKASLPEHALLAEGF
TPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVKAAHGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1134952 ACLVWI_RS16535 WP_411863116.1 3837883..3839592(-) (pilB) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586]
ATCGGCATAACTGGTATTGCTCGTCGATTAGTGCAAGATGGATCACTTGAGGAAGGAGCCGCAAGGTCAGCCATGGATCA
AGCGGCGGCGGCCAAGGTTCCGCTTCCTCAATGGTTTGCAGAAAAAAAACTTGTTAGCGCAGCTCAGCTCGCCGCAGCCA
ATGCAATCGAATTCGGCATGCCTCTGATGGATGTGTCTGTATTCGACGCCAGCCAGAACGCCGTCAAGCTGGTCAGTGAG
GAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCCTGGTCGACGAGGACCAGATCCGTC
GTACCTTGGAGCAGTGGCAAGCCAGCAACGCGTCGTTGGGCTCGTCGCTCGGGGACGATGATGAGGGGATGGGCGACCTG
GACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCATTTCGAACCATATGAAGACGACT
ACCGGGTGCGGCTGCGGATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCGGTGAAGTTGAGCCAGCGTATCGCGGCA
CGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACATTGTTCGGCGAAAAGGTAGTGTTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCG
TACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTTGTATACGGCGCTGGGCATCCTCAATGA
CGAAACGCGCAACATTTCCACCGCGGAGGATCCGGTCGAAATTCGGTTGCCCGGCGTCAACCAGGTGCAGCAGAACAACA
AGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCATTCCTGCGCCAGGACCCGGATATCATCATGGTCGGCGAAATCCGC
GACTTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGTTGTCGACGCTGCACACCAACGATGC
GCCGCAGACCATTGCGCGCCTGATGAACATGGGCATTGCGCCGTACAACATCACCTCGTCGGTGACGTTGGTCATTGCGC
AGCGTCTGGCGCGTCGGTTGTGCAACAACTGCAAGCGCAAGGCGAGTCTGCCCGAGCATGCGTTGTTGGCCGAAGGCTTC
ACGCCGGCGCAGATCGCTGCCGGTATCGAGCTGTACGAGGCGGTCGGTTGCGACGAGTGCACCGAAGGCTACAAGGGCCG
TACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGCAACGCGATGCAGATCG
CCGAGGCGGCGCAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTCAAGGCCGCGCACGGGGTGACCAGCCTG
GCCGAGATCAATCGGGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

99.824

0.557
  pilB Acinetobacter baylyi ADP1

55.106

99.824

0.55
  pilB Legionella pneumophila strain ERS1305867

52.9

100

0.529
  pilF Neisseria gonorrhoeae MS11

48.768

99.824

0.487
  pilB Vibrio cholerae strain A1552

50

97.012

0.485
  pilB Vibrio campbellii strain DS40M4

46.397

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

98.418

0.459
  pilF Thermus thermophilus HB27

39.808

91.388

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.048

92.267

0.36

Multiple sequence alignment