Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLVWI_RS15120 Genome accession   NZ_LR962897
Coordinates   3489036..3490166 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3484036..3495166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLVWI_RS15090 (X12_003013) - 3484426..3484866 (-) 441 WP_016902361.1 OsmC family protein -
  ACLVWI_RS15095 (X12_003014) - 3485077..3486024 (-) 948 WP_024939387.1 aspartate carbamoyltransferase catalytic subunit -
  ACLVWI_RS15100 (X12_003015) ruvX 3486040..3486507 (-) 468 WP_024939388.1 Holliday junction resolvase RuvX -
  ACLVWI_RS15105 (X12_003016) - 3486500..3487066 (-) 567 WP_011051924.1 YqgE/AlgH family protein -
  ACLVWI_RS15110 (X12_003017) - 3487397..3487942 (+) 546 WP_047129056.1 DNA-3-methyladenine glycosylase I -
  ACLVWI_RS15115 (X12_003018) - 3488080..3488754 (-) 675 WP_164741848.1 YitT family protein -
  ACLVWI_RS15120 (X12_003019) pilU 3489036..3490166 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLVWI_RS15125 (X12_003020) pilT 3490289..3491326 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLVWI_RS15130 (X12_003021) - 3491648..3492340 (+) 693 WP_104644730.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLVWI_RS15135 (X12_003022) proC 3492402..3493250 (+) 849 WP_104644731.1 pyrroline-5-carboxylate reductase -
  ACLVWI_RS15140 (X12_003023) - 3493851..3494276 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  ACLVWI_RS15145 (X12_003024) - 3494412..3495032 (+) 621 WP_411861971.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1134947 ACLVWI_RS15120 WP_016902355.1 3489036..3490166(-) (pilU) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1134947 ACLVWI_RS15120 WP_016902355.1 3489036..3490166(-) (pilU) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACCCCGCTGACCGCGCAACAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGTATCCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATTTTTGTCGGTG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTAGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGTTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment