Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CFBP8129_RS17620 Genome accession   NZ_LR828257
Coordinates   4026522..4027652 (-) Length   376 a.a.
NCBI ID   WP_006448884.1    Uniprot ID   A0A0G8P0N2
Organism   Xanthomonas hortorum pv. vitians strain CFBP 498     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4021522..4032652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP8129_RS17590 (CFBP498_36010) - 4021942..4022376 (-) 435 WP_006448877.1 OsmC family protein -
  CFBP8129_RS17595 (CFBP498_36020) - 4022571..4023518 (-) 948 WP_006448878.1 aspartate carbamoyltransferase catalytic subunit -
  CFBP8129_RS17600 (CFBP498_36030) ruvX 4023535..4024002 (-) 468 WP_006448879.1 Holliday junction resolvase RuvX -
  CFBP8129_RS17605 (CFBP498_36040) - 4023995..4024561 (-) 567 WP_074057701.1 YqgE/AlgH family protein -
  CFBP8129_RS17610 (CFBP498_36050) - 4024859..4025404 (+) 546 WP_074057702.1 DNA-3-methyladenine glycosylase I -
  CFBP8129_RS17615 (CFBP498_36060) - 4025546..4026220 (-) 675 WP_043889842.1 YitT family protein -
  CFBP8129_RS17620 (CFBP498_36070) pilU 4026522..4027652 (-) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFBP8129_RS17625 (CFBP498_36080) pilT 4027775..4028812 (-) 1038 WP_006448885.1 type IV pilus twitching motility protein PilT Machinery gene
  CFBP8129_RS17630 (CFBP498_36090) - 4029136..4029828 (+) 693 WP_006448887.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFBP8129_RS17635 (CFBP498_36100) proC 4029876..4030730 (+) 855 WP_074057703.1 pyrroline-5-carboxylate reductase -
  CFBP8129_RS17640 (CFBP498_36110) - 4031169..4031594 (+) 426 WP_043889331.1 HU family DNA-binding protein -
  CFBP8129_RS17645 (CFBP498_36120) - 4031728..4032348 (+) 621 WP_074057704.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41991.26 Da        Isoelectric Point: 6.7684

>NTDB_id=1132404 CFBP8129_RS17620 WP_006448884.1 4026522..4027652(-) (pilU) [Xanthomonas hortorum pv. vitians strain CFBP 498]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1132404 CFBP8129_RS17620 WP_006448884.1 4026522..4027652(-) (pilU) [Xanthomonas hortorum pv. vitians strain CFBP 498]
ATGAGCACTATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACTTCGGGCAT
GCCGCCGGCGATCAAGGTCAACGGCAAGATCAGCCCGATCACCCAGACACCGCTGACGGCGCAACAAAGTCGCGATTTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGTCAGGG
GTCGGGCGTTTTCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGGATTCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTGGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAACACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGTACCCGCGAAGGCA
TGGACCACGCGGTGGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAATTGATTCCGACGCCGGACGGACGCAGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACCCCGTTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGTACCTTC
GATCAGAGCCTGTTCGAGTTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTATGCCGATTCGCAGAACGA
AGTGCGCCTGCGCATCAAACTCTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGGATGGATGGTGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8P0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.667

98.138

0.654
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364

Multiple sequence alignment