Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CFBP498_RS06850 Genome accession   NZ_LR828253
Coordinates   1655410..1656540 (+) Length   376 a.a.
NCBI ID   WP_006448884.1    Uniprot ID   A0A0G8P0N2
Organism   Xanthomonas hortorum pv. gardneri strain CFBP 8129     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1650410..1661540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP498_RS06825 (CFBP8129_13900) - 1650715..1651335 (-) 621 WP_074057704.1 M23 family metallopeptidase -
  CFBP498_RS06830 (CFBP8129_13910) - 1651469..1651894 (-) 426 WP_043889331.1 HU family DNA-binding protein -
  CFBP498_RS06835 (CFBP8129_13920) proC 1652332..1653186 (-) 855 WP_006448888.1 pyrroline-5-carboxylate reductase -
  CFBP498_RS06840 (CFBP8129_13930) - 1653234..1653926 (-) 693 WP_006448887.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFBP498_RS06845 (CFBP8129_13940) pilT 1654250..1655287 (+) 1038 WP_006448885.1 type IV pilus twitching motility protein PilT Machinery gene
  CFBP498_RS06850 (CFBP8129_13950) pilU 1655410..1656540 (+) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFBP498_RS06855 (CFBP8129_13960) - 1656842..1657516 (+) 675 WP_043889842.1 YitT family protein -
  CFBP498_RS06860 (CFBP8129_13970) - 1657658..1658203 (-) 546 WP_006448882.1 DNA-3-methyladenine glycosylase I -
  CFBP498_RS06865 (CFBP8129_13980) - 1658501..1659067 (+) 567 WP_006448880.1 YqgE/AlgH family protein -
  CFBP498_RS06870 (CFBP8129_13990) ruvX 1659060..1659527 (+) 468 WP_006448879.1 Holliday junction resolvase RuvX -
  CFBP498_RS06875 (CFBP8129_14000) - 1659544..1660491 (+) 948 WP_006448878.1 aspartate carbamoyltransferase catalytic subunit -
  CFBP498_RS06880 (CFBP8129_14010) - 1660689..1661123 (+) 435 WP_006448877.1 OsmC family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41991.26 Da        Isoelectric Point: 6.7684

>NTDB_id=1132386 CFBP498_RS06850 WP_006448884.1 1655410..1656540(+) (pilU) [Xanthomonas hortorum pv. gardneri strain CFBP 8129]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1132386 CFBP498_RS06850 WP_006448884.1 1655410..1656540(+) (pilU) [Xanthomonas hortorum pv. gardneri strain CFBP 8129]
ATGAGCACTATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACTTCGGGCAT
GCCGCCGGCGATCAAGGTCAACGGCAAGATCAGCCCGATCACCCAGACACCGCTGACGGCGCAACAAAGTCGCGATTTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGTCAGGG
GTCGGGCGTTTTCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGGATTCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTGGGCG
CCACCGGTACCGGGAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAAGGCA
TGGACCACGCGGTGGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACTCCGGACGGGCGCAGCCGTCGCGTGGCGATGGAGATCATGCTGGGCACCCCGCTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGTTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTATGCCGATTCGCAGAACGA
AGTGCGCCTGCGCATCAAACTCTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGGATGGATGGTGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8P0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.667

98.138

0.654
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364

Multiple sequence alignment