Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H1W95_RS08860 Genome accession   NZ_LR822030
Coordinates   1723120..1724061 (-) Length   313 a.a.
NCBI ID   WP_180483385.1    Uniprot ID   A0AAU9H8P4
Organism   Streptococcus thermophilus isolate STH_CIRM_1046     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1723788..1784241 1723120..1724061 flank -273


Gene organization within MGE regions


Location: 1723120..1784241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W95_RS08860 (STHERMO_2006) comGA/cglA/cilD 1723120..1724061 (-) 942 WP_180483385.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W95_RS08865 (STHERMO_2007) - 1724142..1724504 (-) 363 WP_014608746.1 DUF1033 family protein -
  H1W95_RS08870 (STHERMO_2008) rpoC 1724663..1728301 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -
  H1W95_RS08875 (STHERMO_2009) rpoB 1728402..1731983 (-) 3582 WP_023909983.1 DNA-directed RNA polymerase subunit beta -
  H1W95_RS08880 (STHERMO_2010) pbp1b 1732344..1734767 (-) 2424 WP_180483387.1 penicillin-binding protein PBP1B -
  H1W95_RS08885 (STHERMO_2011) tyrS 1734868..1736124 (+) 1257 WP_011681693.1 tyrosine--tRNA ligase -
  H1W95_RS08890 (STHERMO_2012) ilvC 1736250..1737272 (-) 1023 WP_002952054.1 ketol-acid reductoisomerase -
  H1W95_RS08895 (STHERMO_2013) ilvN 1737348..1737824 (-) 477 WP_011226635.1 acetolactate synthase small subunit -
  H1W95_RS08900 (STHERMO_2014) - 1737817..1739517 (-) 1701 WP_014622011.1 acetolactate synthase large subunit -
  H1W95_RS08905 (STHERMO_2015) ilvD 1739652..1741372 (-) 1721 Protein_1718 dihydroxy-acid dehydratase -
  H1W95_RS08910 (STHERMO_2017) - 1741542..1743209 (-) 1668 WP_014608752.1 DAK2 domain-containing protein -
  H1W95_RS08915 (STHERMO_2018) - 1743209..1743574 (-) 366 WP_011681698.1 Asp23/Gls24 family envelope stress response protein -
  H1W95_RS08920 (STHERMO_2019) - 1743684..1744973 (-) 1290 WP_011681699.1 MATE family efflux transporter -
  H1W95_RS08925 (STHERMO_2020) thrC 1745031..1746515 (-) 1485 WP_014608753.1 threonine synthase -
  H1W95_RS10375 adhE 1747103..1749695 (-) 2593 Protein_1723 bifunctional acetaldehyde-CoA/alcohol dehydrogenase -
  H1W95_RS08960 (STHERMO_2026) - 1749834..1751729 (-) 1896 WP_014608759.1 M13 family metallopeptidase -
  H1W95_RS10380 - 1751810..1752962 (-) 1153 Protein_1725 alpha-amylase family glycosyl hydrolase -
  H1W95_RS10750 (STHERMO_2031) - 1753029..1753345 (-) 317 Protein_1726 PTS sugar transporter subunit IIA -
  H1W95_RS10755 (STHERMO_2032) - 1753400..1753765 (-) 366 WP_014608761.1 PTS trehalose transporter subunit IIBC -
  H1W95_RS08990 (STHERMO_2035) treR 1754111..1754821 (+) 711 Protein_1728 trehalose operon repressor -
  H1W95_RS08995 (STHERMO_2036) - 1754877..1755437 (-) 561 WP_002948266.1 TIGR01440 family protein -
  H1W95_RS09000 (STHERMO_2037) - 1755450..1755788 (-) 339 WP_041829338.1 hypothetical protein -
  H1W95_RS09005 (STHERMO_2038) - 1755821..1756177 (-) 357 WP_164135671.1 hypothetical protein -
  H1W95_RS10385 (STHERMO_2039) - 1756441..1756728 (+) 288 WP_224102980.1 helix-turn-helix domain-containing protein -
  H1W95_RS09010 (STHERMO_2040) - 1756745..1757431 (+) 687 WP_232087022.1 IS30 family transposase -
  H1W95_RS09015 (STHERMO_2041) - 1757849..1758631 (-) 783 WP_180483390.1 toll/interleukin-1 receptor domain-containing protein -
  H1W95_RS09860 (STHERMO_2042) - 1758791..1759558 (+) 768 WP_197926869.1 DUF262 domain-containing protein -
  H1W95_RS09865 (STHERMO_2043) - 1759515..1759811 (+) 297 WP_197926870.1 hypothetical protein -
  H1W95_RS10530 (STHERMO_2044) - 1759815..1760279 (+) 465 WP_197926871.1 GmrSD restriction endonuclease domain-containing protein -
  H1W95_RS09875 (STHERMO_2045) - 1760263..1760628 (+) 366 WP_197926872.1 GmrSD restriction endonuclease domain-containing protein -
  H1W95_RS09025 (STHERMO_2047) - 1761255..1761740 (+) 486 WP_232087023.1 hypothetical protein -
  H1W95_RS09030 (STHERMO_2048) - 1761757..1762059 (+) 303 WP_167401585.1 hypothetical protein -
  H1W95_RS10760 - 1762220..1762360 (+) 141 WP_180483615.1 helix-turn-helix domain-containing protein -
  H1W95_RS09040 (STHERMO_2050) - 1762353..1763450 (+) 1098 WP_179959070.1 DNA cytosine methyltransferase -
  H1W95_RS09045 (STHERMO_2051) - 1763437..1764663 (+) 1227 WP_232087024.1 DNA cytosine methyltransferase -
  H1W95_RS09050 (STHERMO_2052) - 1764688..1765731 (-) 1044 WP_180483392.1 restriction endonuclease PLD domain-containing protein -
  H1W95_RS09055 (STHERMO_2054) - 1765924..1766829 (-) 906 WP_180483618.1 restriction endonuclease PLD domain-containing protein -
  H1W95_RS09060 - 1766933..1767720 (-) 788 Protein_1746 ISL3 family transposase -
  H1W95_RS09065 - 1767848..1768105 (-) 258 Protein_1747 hypothetical protein -
  H1W95_RS09070 (STHERMO_2057) - 1768255..1768941 (-) 687 WP_180483395.1 helix-turn-helix domain-containing protein -
  H1W95_RS09075 (STHERMO_2058) - 1768950..1769810 (-) 861 WP_180483397.1 ImmA/IrrE family metallo-endopeptidase -
  H1W95_RS09080 (STHERMO_2060) - 1770303..1770491 (-) 189 WP_180483399.1 helix-turn-helix transcriptional regulator -
  H1W95_RS09085 (STHERMO_2061) - 1770497..1770838 (-) 342 WP_180483401.1 helix-turn-helix domain-containing protein -
  H1W95_RS09090 (STHERMO_2063) - 1771701..1771997 (+) 297 WP_073945520.1 cytoplasmic protein -
  H1W95_RS09095 (STHERMO_2064) - 1772019..1772480 (+) 462 WP_084831216.1 conjugal transfer protein -
  H1W95_RS09100 (STHERMO_2065) - 1772494..1774182 (+) 1689 WP_180483403.1 FtsK/SpoIIIE domain-containing protein -
  H1W95_RS09105 (STHERMO_2066) - 1774390..1775622 (+) 1233 WP_180483405.1 replication initiation factor domain-containing protein -
  H1W95_RS09110 (STHERMO_2067) - 1775637..1775867 (+) 231 WP_021144867.1 hypothetical protein -
  H1W95_RS09115 (STHERMO_2068) - 1775871..1776428 (+) 558 WP_045769331.1 hypothetical protein -
  H1W95_RS09120 (STHERMO_2069) - 1776440..1777435 (+) 996 WP_180483407.1 conjugal transfer protein -
  H1W95_RS09125 (STHERMO_2070) - 1777445..1777669 (+) 225 WP_084831221.1 hypothetical protein -
  H1W95_RS09130 (STHERMO_2071) - 1777672..1778082 (+) 411 WP_180483409.1 conjugal transfer protein -
  H1W95_RS09135 (STHERMO_2072) - 1778096..1780600 (+) 2505 WP_180483411.1 ATP-binding protein -
  H1W95_RS09140 (STHERMO_2073) - 1780612..1782492 (+) 1881 WP_180483413.1 conjugal transfer protein -
  H1W95_RS09145 (STHERMO_2074) - 1782494..1782718 (+) 225 WP_155222550.1 hypothetical protein -
  H1W95_RS09150 (STHERMO_2075) - 1782744..1783856 (+) 1113 WP_180483415.1 CHAP domain-containing protein -
  H1W95_RS09155 (STHERMO_2076) - 1783870..1784118 (+) 249 WP_037611155.1 hypothetical protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35552.76 Da        Isoelectric Point: 6.8365

>NTDB_id=1131492 H1W95_RS08860 WP_180483385.1 1723120..1724061(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1046]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVARMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKRDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGISYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1131492 H1W95_RS08860 WP_180483385.1 1723120..1724061(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1046]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGAGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACGGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTTCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

64.952

99.361

0.645
  comYA Streptococcus mutans UA159

64.217

100

0.642
  comYA Streptococcus mutans UA140

64.217

100

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.666

99.361

0.633
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.666

99.361

0.633
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.666

99.361

0.633
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.666

99.361

0.633
  comYA Streptococcus gordonii str. Challis substr. CH1

62.62

100

0.626
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.577

99.681

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505
  comGA Latilactobacillus sakei subsp. sakei 23K

38.158

97.125

0.371

Multiple sequence alignment