Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1Z78_RS05730 Genome accession   NZ_LR813084
Coordinates   1272878..1274620 (-) Length   580 a.a.
NCBI ID   WP_234640593.1    Uniprot ID   -
Organism   Delftia tsuruhatensis isolate BB1455     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1267878..1279620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1Z78_RS05700 (TML_01138) - 1268509..1269000 (+) 492 WP_234640587.1 NUDIX domain-containing protein -
  L1Z78_RS05705 (TML_01139) - 1269001..1269225 (-) 225 WP_234640588.1 DNA gyrase inhibitor YacG -
  L1Z78_RS05710 (TML_01140) zapD 1269243..1269998 (-) 756 WP_234640589.1 cell division protein ZapD -
  L1Z78_RS05715 (TML_01141) coaE 1270088..1270726 (-) 639 WP_234640590.1 dephospho-CoA kinase -
  L1Z78_RS05720 (TML_01142) pilD 1270741..1271622 (-) 882 WP_234640591.1 prepilin peptidase Machinery gene
  L1Z78_RS05725 (TML_01143) pilC 1271622..1272839 (-) 1218 WP_234640592.1 type II secretion system F family protein Machinery gene
  L1Z78_RS05730 (TML_01144) pilB 1272878..1274620 (-) 1743 WP_234640593.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L1Z78_RS05740 (TML_01146) - 1274869..1275798 (-) 930 WP_234642099.1 polyprenyl synthetase family protein -
  L1Z78_RS05745 (TML_01147) rplU 1276030..1276341 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  L1Z78_RS05750 (TML_01148) rpmA 1276357..1276614 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  L1Z78_RS05755 (TML_01149) cgtA 1276773..1277888 (+) 1116 WP_234640594.1 Obg family GTPase CgtA -
  L1Z78_RS05760 (TML_01150) proB 1277972..1279120 (+) 1149 WP_234640595.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62699.82 Da        Isoelectric Point: 5.1506

>NTDB_id=1130382 L1Z78_RS05730 WP_234640593.1 1272878..1274620(-) (pilB) [Delftia tsuruhatensis isolate BB1455]
MAVADSSLKNASAVASLPGIGRALVSAGKVSQQAAEAAASKAVANRSSFIAELSSAGTISALEVAETVSTMFSAPLLDLD
AIDPMRLPQELLDTKISTTYRVLPLSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLDRLIAQLNKSAGEA
MESYGGGGDFEFGDLKTDEPAEEKDNTAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLLDAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLDAGYADAEVDGSWVTYKPVGCPACNNGYKGRVGIYQVMPISEDIQRIILQDGSALEIAAQARREGVRSLREAG
LHKARMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1130382 L1Z78_RS05730 WP_234640593.1 1272878..1274620(-) (pilB) [Delftia tsuruhatensis isolate BB1455]
ATGGCTGTTGCTGACTCCTCGCTCAAGAACGCTTCCGCTGTCGCATCGCTGCCAGGCATAGGGCGCGCCCTGGTCTCTGC
CGGCAAGGTATCGCAGCAGGCAGCGGAAGCAGCCGCCAGCAAGGCCGTCGCCAACCGCAGTTCGTTCATCGCGGAACTGA
GCAGCGCAGGCACCATTTCCGCACTGGAAGTGGCAGAGACCGTCTCTACCATGTTCAGCGCGCCGCTGCTCGATCTCGAT
GCCATCGACCCGATGCGGCTGCCGCAGGAACTGCTGGACACCAAGATCAGCACGACCTACCGCGTCCTGCCGCTGAGCAA
GCGCGGCAACCGCCTCACGGTGGCCACGGCCGACCCCACGCAGCAGGAAGCCGCGGAACAGATCAAGTTCACCACGCAGC
TTGGTGTGGACTGGATCATCGTCGAGGCTGACAAGCTGGACAGGCTGATAGCGCAACTGAACAAAAGTGCGGGCGAAGCC
ATGGAAAGCTATGGCGGCGGCGGCGACTTCGAGTTCGGCGACCTCAAGACCGACGAACCCGCCGAGGAAAAGGACAACAC
GGCGGCGGCGGACGTCGAAGACGCACCCATCGTCAAGTTCCTGCACAAGATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACTTGCACTTCGAACCCTATGAGCATCAGTACCGCGTGCGCTTTCGTATCGACGGCGAGTTGCGCGAGATTGCCTCG
CCGCCGATTGCCATCAAGGAAAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAAAAGCGTGTTCC
CCAGGACGGACGCATGAAGCTGAAGGTCGGTCCGGACCGTGTCATCGATTTCCGCGTCAGCACGCTGCCCACGCTGTTCG
GCGAAAAGATCGTGATCCGTATCCTGGATCCCAGTTCTGCCAAGATGGGCATCGATGCCCTGGGCTACGAGCCCGAGGAG
AAGGCACGCCTGCTTGATGCCATCGGCCGCCCCTACGGCATGATCCTGGTCACCGGACCCACGGGCTCGGGCAAGACCGT
GTCGCTCTACACCTGCCTGAACCTGCTGAACAAGCCCGGGGTGAACATCGCGACGGCCGAGGACCCTTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTGAACGAGAAGGCCGGCCTCACCTTCGCGACGGCGCTCAAGGCCTTTCTGCGCCAG
GATCCGGACATCATCATGGTCGGCGAAATCCGGGACCTGGAGACTGCCGATATTTCGATCAAGGCCGCCCAGACCGGCCA
CTTGGTGCTGTCCACGCTGCACACCAATGACGCGCCCACCACGCTGACCCGCATGCGCAACATGGGCATCGCGCCATTCA
ACATCGCCTCCAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCAGGCGGCTGTGCCCCCAGTGCAAGGAACCCGCCGAC
ATTCCCCGGGAGGCCTTGCTCGACGCGGGCTATGCCGACGCGGAAGTCGACGGCAGCTGGGTCACCTACAAACCCGTCGG
CTGCCCGGCCTGCAACAATGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAGGACATCCAGCGCA
TCATCCTGCAGGACGGCAGCGCCCTGGAGATCGCGGCCCAGGCCAGGCGCGAAGGCGTGCGATCGCTGCGCGAAGCCGGC
CTGCACAAAGCCCGGATGGGACTGACCTCACTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.009

98.103

0.54
  pilB Acinetobacter baumannii D1279779

55.142

97.241

0.536
  pilF Neisseria gonorrhoeae MS11

52.297

97.586

0.51
  pilB Legionella pneumophila strain ERS1305867

49.735

97.414

0.484
  pilB Vibrio cholerae strain A1552

46.071

96.552

0.445
  pilB Vibrio campbellii strain DS40M4

45.259

96.379

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

92.241

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.529

98.448

0.379
  pilF Thermus thermophilus HB27

38.556

97.931

0.378

Multiple sequence alignment