Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BAMY6639_RS03425 Genome accession   NZ_CP006058
Coordinates   750438..751052 (-) Length   204 a.a.
NCBI ID   WP_061860722.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens UMAF6639     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745438..756052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMY6639_RS03405 (BAMY6639_03590) rpsT 745741..746007 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  BAMY6639_RS03410 (BAMY6639_03595) holA 746024..747065 (-) 1042 Protein_704 DNA polymerase III subunit delta -
  BAMY6639_RS20040 - 747105..747256 (-) 152 Protein_705 hypothetical protein -
  BAMY6639_RS19525 (BAMY6639_03600) - 747297..747431 (+) 135 WP_003152870.1 YqzM family protein -
  BAMY6639_RS03415 (BAMY6639_03605) comEC 747450..749801 (-) 2352 WP_060674655.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BAMY6639_RS03420 (BAMY6639_03610) - 749802..750371 (-) 570 WP_003152868.1 ComE operon protein 2 -
  BAMY6639_RS03425 (BAMY6639_03615) comEA 750438..751052 (-) 615 WP_061860722.1 helix-hairpin-helix domain-containing protein Machinery gene
  BAMY6639_RS03430 (BAMY6639_03620) comER 751111..751932 (+) 822 WP_061860723.1 late competence protein ComER -
  BAMY6639_RS03435 (BAMY6639_03625) - 752001..752738 (-) 738 WP_015417856.1 class I SAM-dependent DNA methyltransferase -
  BAMY6639_RS03440 (BAMY6639_03630) rsfS 752735..753091 (-) 357 WP_007408260.1 ribosome silencing factor -
  BAMY6639_RS03445 (BAMY6639_03635) yqeK 753115..753669 (-) 555 WP_061860724.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BAMY6639_RS03450 (BAMY6639_03640) - 753659..754228 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  BAMY6639_RS03455 (BAMY6639_03645) yhbY 754239..754529 (-) 291 WP_061860725.1 ribosome assembly RNA-binding protein YhbY -
  BAMY6639_RS03460 (BAMY6639_03650) aroE 754523..755365 (-) 843 WP_039254404.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22263.50 Da        Isoelectric Point: 6.4888

>NTDB_id=112953 BAMY6639_RS03425 WP_061860722.1 750438..751052(-) (comEA) [Bacillus amyloliquefaciens UMAF6639]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNINLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=112953 BAMY6639_RS03425 WP_061860722.1 750438..751052(-) (comEA) [Bacillus amyloliquefaciens UMAF6639]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATAT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.143

96.078

0.549
  comEA Lactococcus lactis subsp. cremoris KW2

35.586

100

0.387
  comEA Latilactobacillus sakei subsp. sakei 23K

33.19

100

0.377
  comEA/celA/cilE Streptococcus mitis NCTC 12261

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae Rx1

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae D39

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae R6

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

36.493

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment