Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   FGL14_RS00690 Genome accession   NZ_LR594045
Coordinates   98989..99930 (+) Length   313 a.a.
NCBI ID   WP_138083656.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC9413     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93989..104930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL14_RS00680 (NCTC9413_00140) rpoC 94699..98340 (+) 3642 WP_037589802.1 DNA-directed RNA polymerase subunit beta' -
  FGL14_RS00685 (NCTC9413_00141) - 98543..98908 (+) 366 WP_003055257.1 DUF1033 family protein -
  FGL14_RS00690 (NCTC9413_00142) comYA 98989..99930 (+) 942 WP_138083656.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL14_RS00695 (NCTC9413_00143) comYB 99866..100897 (+) 1032 WP_138084099.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL14_RS00700 (NCTC9413_00144) comYC 100899..101225 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL14_RS00705 (NCTC9413_00145) comGD 101200..101628 (+) 429 WP_041788719.1 competence type IV pilus minor pilin ComGD -
  FGL14_RS00710 (NCTC9413_00146) comGE 101636..101881 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  FGL14_RS00715 (NCTC9413_00147) comGF 101862..102302 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  FGL14_RS00720 (NCTC9413_00148) comGG 102331..102654 (+) 324 WP_227985720.1 competence type IV pilus minor pilin ComGG -
  FGL14_RS00725 (NCTC9413_00149) comYH 102717..103670 (+) 954 WP_138083657.1 class I SAM-dependent methyltransferase Machinery gene
  FGL14_RS00730 (NCTC9413_00150) - 103729..104925 (+) 1197 WP_138083658.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35192.31 Da        Isoelectric Point: 7.2467

>NTDB_id=1127716 FGL14_RS00690 WP_138083656.1 98989..99930(+) (comYA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC9413]
MVQALARSILKEAEQIQAQDIYILPKEDQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGYLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1127716 FGL14_RS00690 WP_138083656.1 98989..99930(+) (comYA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC9413]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AGATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGTAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGATATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.361

0.613
  comYA Streptococcus mutans UA159

61.736

99.361

0.613
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

99.042

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.361

0.604
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

51.768

99.361

0.514
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364

Multiple sequence alignment