Detailed information NTG1126259

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: E0F31_RS04650
• Genome accession: NZ_LR216064
• Coordinates: 864794..865444 (+)
• Length: 216 a.a.
• NCBI ID: WP_050289035.1
• Uniprot ID: A0A4G4S6Q6
• Organism: Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 859794..870444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F31_RS04620 (SAMEA3714520_00862)	pyrH	860518..861255 (+)	738	WP_000002998.1	UMP kinase	-
E0F31_RS04625 (SAMEA3714520_00863)	frr	861264..861821 (+)	558	WP_001262225.1	ribosome recycling factor	-
E0F31_RS04630 (SAMEA3714520_00864)	cvfB	861881..862735 (+)	855	WP_001095438.1	RNA-binding virulence regulatory protein CvfB	-
E0F31_RS04635 (SAMEA3714520_00865)	-	862744..862959 (+)	216	WP_001232082.1	YozE family protein	-
E0F31_RS04640 (SAMEA3714520_00866)	-	863045..864049 (+)	1005	WP_050096410.1	PhoH family protein	-
E0F31_RS04645 (SAMEA3714520_00867)	-	864157..864726 (+)	570	WP_000443770.1	GNAT family N-acetyltransferase	-
E0F31_RS04650 (SAMEA3714520_00868)	comEA/celA/cilE	864794..865444 (+)	651	WP_050289035.1	ComEA family DNA-binding protein	Machinery gene
E0F31_RS04655 (SAMEA3714520_00869)	comEC/celB	865428..867668 (+)	2241	WP_054363167.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E0F31_RS04660	-	867847..868035 (+)	189	WP_044812623.1	hypothetical protein	-
E0F31_RS04665 (SAMEA3714520_00870)	-	868068..868655 (+)	588	WP_000939884.1	ATP-binding cassette domain-containing protein	-
E0F31_RS04670 (SAMEA3714520_00871)	-	868659..869843 (+)	1185	WP_050289032.1	ABC transporter permease	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23202.63 Da
• Isoelectric Point: 6.9977

>NTDB_id=1126259 E0F31_RS04650 WP_050289035.1 864794..865444(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MEAIIKKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSIDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1126259 E0F31_RS04650 WP_050289035.1 864794..865444(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGGAAGCAATTATCAAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCAGTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAGCTTAAACAGGTTAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATAGACCATCGTGAGGCAAATGG
GAAATTCAAGTCGATTGATGAACTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A4G4S6Q6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae D39	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae R6	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis NCTC 12261	94.907	100	0.949
comEA/celA/cilE	Streptococcus mitis SK321	90.278	100	0.903
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Bacillus subtilis subsp. subtilis str. 168	42.105	87.963	0.37
comEA	Latilactobacillus sakei subsp. sakei 23K	33.906	100	0.366