Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC/celB   Type   Machinery gene
Locus tag   E0F31_RS04655 Genome accession   NZ_LR216064
Coordinates   865428..867668 (+) Length   746 a.a.
NCBI ID   WP_054363167.1    Uniprot ID   A0A4J1X0C6
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 860428..872668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS04620 (SAMEA3714520_00862) pyrH 860518..861255 (+) 738 WP_000002998.1 UMP kinase -
  E0F31_RS04625 (SAMEA3714520_00863) frr 861264..861821 (+) 558 WP_001262225.1 ribosome recycling factor -
  E0F31_RS04630 (SAMEA3714520_00864) cvfB 861881..862735 (+) 855 WP_001095438.1 RNA-binding virulence regulatory protein CvfB -
  E0F31_RS04635 (SAMEA3714520_00865) - 862744..862959 (+) 216 WP_001232082.1 YozE family protein -
  E0F31_RS04640 (SAMEA3714520_00866) - 863045..864049 (+) 1005 WP_050096410.1 PhoH family protein -
  E0F31_RS04645 (SAMEA3714520_00867) - 864157..864726 (+) 570 WP_000443770.1 GNAT family N-acetyltransferase -
  E0F31_RS04650 (SAMEA3714520_00868) comEA/celA/cilE 864794..865444 (+) 651 WP_050289035.1 ComEA family DNA-binding protein Machinery gene
  E0F31_RS04655 (SAMEA3714520_00869) comEC/celB 865428..867668 (+) 2241 WP_054363167.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  E0F31_RS04660 - 867847..868035 (+) 189 WP_044812623.1 hypothetical protein -
  E0F31_RS04665 (SAMEA3714520_00870) - 868068..868655 (+) 588 WP_000939884.1 ATP-binding cassette domain-containing protein -
  E0F31_RS04670 (SAMEA3714520_00871) - 868659..869843 (+) 1185 WP_050289032.1 ABC transporter permease -
  E0F31_RS04675 (SAMEA3714520_00872) infC 870154..870684 (+) 531 WP_000848180.1 translation initiation factor IF-3 -
  E0F31_RS04680 (SAMEA3714520_00873) rpmI 870717..870917 (+) 201 WP_001125943.1 50S ribosomal protein L35 -
  E0F31_RS04685 (SAMEA3714520_00874) rplT 870969..871328 (+) 360 WP_000124836.1 50S ribosomal protein L20 -
  E0F31_RS04690 (SAMEA3714520_00875) - 871386..871766 (+) 381 WP_000157154.1 VOC family protein -

Sequence

Protein


Download         Length: 746 a.a.        Molecular weight: 84718.29 Da        Isoelectric Point: 9.3723

>NTDB_id=1126260 E0F31_RS04655 WP_054363167.1 865428..867668(+) (comEC/celB) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCLFIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQN
LVDSVERVRILPDTIKVNGDSLSFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLTFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLISLALVYDLRKNIKKLTVLCLLITGLFLLTK
HPLENEITMLDVGQGESIFLWDITGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
EHVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKTSQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide


Download         Length: 2241 bp        

>NTDB_id=1126260 E0F31_RS04655 WP_054363167.1 865428..867668(+) (comEC/celB) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTGTTACTATGGCTTTACTACGCCATTTT
CTCAGTATCCTATTTTGCTTTGTTGGGTTTTGTTTTTCTGCTAGTCTGCCTCTTTATCCAATTTCCTTGGAAATCAGCAG
GTAAAGTTCTAGTGATTTGTGGAGTCTTTGGCTTCTGGTTTCTGTTTCAAACTTGGCAACAGAGTCAAGTGAGTCAAAAT
TTGGTGGATTCTGTTGAAAGGGTACGGATTTTACCAGACACTATTAAGGTCAACGGTGACAGTCTGTCCTTTCGTGGTAA
GTCTGATGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACTGACC
TTCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTGACTACCAAGGCTAT
CTGAAGACTCAGGGAATTTACCAGACACTCAATATTAAAAGAATCCAGTCACTCCAAAAGGTTGGTAGTTGGGATATAGG
TGAAAACCTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGATGCACTTCCCAGACCCTATGCGCAATTACATGA
CAGGACTTTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGCATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTAACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTGCTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTATTTGACTTGACCTTCTTACCGCTCTTGTCTATT
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTGGAGGGCATTATTCGCTTGGT
GTCACAGGTGACAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGTTTTTAATCCTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGAAAAAACATTAAAAAGCTAACGGTATTGTGCTTATTGATTACAGGGCTCTTTCTCCTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTATGGGATATAACTGGGAAAAC
CATTCTCATAGATGTCGGTGGCAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGCATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAACTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

Competence protein (Competence)

(216-458)

Domain of unknown function (DUF4131) (DUF4131)

(30-176)

Metallo-beta-lactamase superfamily (Lactamase_B)

(492-697)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4J1X0C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus pneumoniae Rx1

96.381

100

0.964
  comEC/celB Streptococcus pneumoniae D39

96.381

100

0.964
  comEC/celB Streptococcus pneumoniae R6

96.381

100

0.964
  comEC/celB Streptococcus pneumoniae TIGR4

95.979

100

0.96
  comEC/celB Streptococcus mitis SK321

91.421

100

0.914
  comEC/celB Streptococcus mitis NCTC 12261

91.409

99.866

0.913
  comEC Lactococcus lactis subsp. cremoris KW2

44.399

99.33

0.441

Multiple sequence alignment