Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL230_RS03850 Genome accession   NZ_LR134490
Coordinates   757157..758551 (+) Length   464 a.a.
NCBI ID   WP_126513488.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC11873     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 752157..763551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL230_RS03825 (NCTC11873_00760) rsmE 752173..752910 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EL230_RS03830 (NCTC11873_00761) lnt 752960..754489 (-) 1530 WP_126513487.1 apolipoprotein N-acyltransferase -
  EL230_RS03835 (NCTC11873_00762) corC 754512..755411 (-) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  EL230_RS03840 (NCTC11873_00763) ampD 756043..756597 (-) 555 WP_041175038.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL230_RS03845 (NCTC11873_00764) pilA 756711..757160 (+) 450 WP_015701561.1 prepilin peptidase-dependent pilin Machinery gene
  EL230_RS03850 (NCTC11873_00765) pilB 757157..758551 (+) 1395 WP_126513488.1 GspE/PulE family protein Machinery gene
  EL230_RS03855 (NCTC11873_00766) pilC 758548..759768 (+) 1221 WP_126513489.1 type II secretion system F family protein Machinery gene
  EL230_RS03860 (NCTC11873_00767) pilD 759765..760457 (+) 693 WP_126513490.1 prepilin peptidase Machinery gene
  EL230_RS03865 (NCTC11873_00768) rho 760511..761773 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  EL230_RS03870 (NCTC11873_00769) metJ 762018..762335 (+) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  EL230_RS03875 (NCTC11873_00770) cueR 762349..762735 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  EL230_RS03880 (NCTC11873_00771) - 762812..763018 (+) 207 WP_012054944.1 heavy-metal-associated domain-containing protein -
  EL230_RS03885 (NCTC11873_00772) - 763092..763298 (+) 207 WP_012054944.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52921.50 Da        Isoelectric Point: 5.9833

>NTDB_id=1123199 EL230_RS03850 WP_126513488.1 757157..758551(+) (pilB) [Haemophilus influenzae strain NCTC11873]
MTSYALLHTQRVTAKNGEVFTISPDLWERNRQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELGGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLRESGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1123199 EL230_RS03850 WP_126513488.1 757157..758551(+) (pilB) [Haemophilus influenzae strain NCTC11873]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCGGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCTTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAGCCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACACGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGGTGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTGCGTGC
TGCCCAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGCTTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAATAGCTTATTGCTCGTTATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACCGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

97.414

100

0.974
  pilB Haemophilus influenzae 86-028NP

97.408

99.784

0.972
  pilB Glaesserella parasuis strain SC1401

58.333

98.276

0.573
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.763

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment