Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL190_RS04520 Genome accession   NZ_LR134395
Coordinates   842762..844156 (+) Length   464 a.a.
NCBI ID   WP_006996185.1    Uniprot ID   -
Organism   Haemophilus aegyptius strain NCTC8134     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 837762..849156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL190_RS04495 (NCTC8134_00919) rsmE 837780..838517 (-) 738 WP_006996180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EL190_RS04500 (NCTC8134_00920) lnt 838567..840096 (-) 1530 WP_013525535.1 apolipoprotein N-acyltransferase -
  EL190_RS04505 (NCTC8134_00921) corC 840119..841018 (-) 900 WP_006996182.1 CNNM family magnesium/cobalt transport protein CorC -
  EL190_RS04510 (NCTC8134_00922) ampD 841641..842201 (-) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL190_RS04515 (NCTC8134_00923) pilA 842316..842765 (+) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL190_RS04520 (NCTC8134_00924) pilB 842762..844156 (+) 1395 WP_006996185.1 GspE/PulE family protein Machinery gene
  EL190_RS04525 (NCTC8134_00925) pilC 844153..845370 (+) 1218 WP_006996186.1 type II secretion system F family protein Machinery gene
  EL190_RS04530 (NCTC8134_00926) pilD 845367..846059 (+) 693 WP_006996187.1 prepilin peptidase Machinery gene
  EL190_RS04535 (NCTC8134_00927) rho 846115..847377 (-) 1263 WP_006996188.1 transcription termination factor Rho -
  EL190_RS04540 (NCTC8134_00928) metJ 847612..847929 (+) 318 WP_006996189.1 met regulon transcriptional regulator MetJ -
  EL190_RS04545 (NCTC8134_00929) cueR 847943..848329 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  EL190_RS04550 (NCTC8134_00930) - 848406..848612 (+) 207 WP_005654130.1 heavy-metal-associated domain-containing protein -
  EL190_RS04555 (NCTC8134_00931) - 848698..848904 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52936.41 Da        Isoelectric Point: 5.4883

>NTDB_id=1122731 EL190_RS04520 WP_006996185.1 842762..844156(+) (pilB) [Haemophilus aegyptius strain NCTC8134]
MTSYALLHTQRVTAQNGEIFTISPDLWVRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1122731 EL190_RS04520 WP_006996185.1 842762..844156(+) (pilB) [Haemophilus aegyptius strain NCTC8134]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCTGATTTATG
GGTGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATAGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCGATGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGGGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.922

100

0.989
  pilB Haemophilus influenzae Rd KW20

96.76

99.784

0.966
  pilB Glaesserella parasuis strain SC1401

57.895

98.276

0.569
  pilB Vibrio cholerae strain A1552

40.038

100

0.455
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.877

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.853

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment