Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EL190_RS04530 Genome accession   NZ_LR134395
Coordinates   845367..846059 (+) Length   230 a.a.
NCBI ID   WP_006996187.1    Uniprot ID   -
Organism   Haemophilus aegyptius strain NCTC8134     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 840367..851059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL190_RS04510 (NCTC8134_00922) ampD 841641..842201 (-) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL190_RS04515 (NCTC8134_00923) pilA 842316..842765 (+) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL190_RS04520 (NCTC8134_00924) pilB 842762..844156 (+) 1395 WP_006996185.1 GspE/PulE family protein Machinery gene
  EL190_RS04525 (NCTC8134_00925) pilC 844153..845370 (+) 1218 WP_006996186.1 type II secretion system F family protein Machinery gene
  EL190_RS04530 (NCTC8134_00926) pilD 845367..846059 (+) 693 WP_006996187.1 prepilin peptidase Machinery gene
  EL190_RS04535 (NCTC8134_00927) rho 846115..847377 (-) 1263 WP_006996188.1 transcription termination factor Rho -
  EL190_RS04540 (NCTC8134_00928) metJ 847612..847929 (+) 318 WP_006996189.1 met regulon transcriptional regulator MetJ -
  EL190_RS04545 (NCTC8134_00929) cueR 847943..848329 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  EL190_RS04550 (NCTC8134_00930) - 848406..848612 (+) 207 WP_005654130.1 heavy-metal-associated domain-containing protein -
  EL190_RS04555 (NCTC8134_00931) - 848698..848904 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  EL190_RS04560 (NCTC8134_00932) - 848879..851050 (+) 2172 WP_006996190.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26761.78 Da        Isoelectric Point: 8.8544

>NTDB_id=1122733 EL190_RS04530 WP_006996187.1 845367..846059(+) (pilD) [Haemophilus aegyptius strain NCTC8134]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYVELFPQNFLSFQPSVSSIQQKKCGHILRYFLSVGVGFIFLQIAF
KDSIFTVWFGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYWY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=1122733 EL190_RS04530 WP_006996187.1 845367..846059(+) (pilD) [Haemophilus aegyptius strain NCTC8134]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTTTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACCTACGTTGAATTATTTCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAGTGTTGGAGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGTTCGGACTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGGGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATAATTTATTTCGTCAAATATTACTGGTATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

91.739

100

0.917
  pilD Haemophilus influenzae Rd KW20

91.304

100

0.913

Multiple sequence alignment