Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL249_RS04285 Genome accession   NZ_LR134378
Coordinates   1055510..1057309 (-) Length   599 a.a.
NCBI ID   WP_005674124.1    Uniprot ID   -
Organism   Lautropia mirabilis strain NCTC12852     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1050510..1062309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL249_RS04260 (NCTC12852_00846) - 1051040..1051261 (-) 222 WP_005674129.1 DNA gyrase inhibitor YacG -
  EL249_RS04265 (NCTC12852_00847) zapD 1051261..1052016 (-) 756 WP_005674128.1 cell division protein ZapD -
  EL249_RS04270 (NCTC12852_00848) coaE 1052212..1052901 (-) 690 WP_005674127.1 dephospho-CoA kinase -
  EL249_RS04275 (NCTC12852_00849) pilD 1053053..1053970 (-) 918 WP_005674126.1 prepilin peptidase Machinery gene
  EL249_RS04280 (NCTC12852_00850) pilC 1054182..1055429 (-) 1248 WP_005674125.1 type II secretion system F family protein Machinery gene
  EL249_RS04285 (NCTC12852_00851) pilB 1055510..1057309 (-) 1800 WP_005674124.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL249_RS04290 (NCTC12852_00852) - 1057486..1058454 (-) 969 WP_040531668.1 Dyp-type peroxidase -
  EL249_RS04295 (NCTC12852_00853) - 1059079..1060377 (+) 1299 WP_005674120.1 MFS transporter -
  EL249_RS04300 (NCTC12852_00854) - 1060404..1061813 (+) 1410 WP_005674119.1 NarK family nitrate/nitrite MFS transporter -

Sequence

Protein


Download         Length: 599 a.a.        Molecular weight: 65574.42 Da        Isoelectric Point: 5.0098

>NTDB_id=1122465 EL249_RS04285 WP_005674124.1 1055510..1057309(-) (pilB) [Lautropia mirabilis strain NCTC12852]
MDPSRLFLIHEGRLPVSANLPPQAKNERADKADAVQLPGIARVLIQHQLLSPEQAREAHAKAQAAENGNFLDELVINSKV
LNPLRLATFLAETFGMPLMDLASLDFSTLPLDQIDRKLLAETRVIPLIKRGNRLAVLLSDPTDNQAIERVKFQQQAIIDP
IVVEHSKLVKLIQNLDQSTADKLADLIGDVEEEESAVADGAVALEDNADVDDAPIVRFLQKVLMDAIQGGASDIHFEPYE
KFYRIRFRVDGELREITQPPLVIKDKLASRIKVISRLDISEKRVPQDGRMKLVISNNRAIDFRVSTLPTLFGEKIVMRIL
DPSSAKLGIDALGYDPEQKKALTEAIARPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNIATAEDPAEINLPGINQVNV
NDKAGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILI
TAQRLARRLCTQCKQPVDLDDDALLAAGFTEDDLDGSWRPYKAVGCERCNGSGYKGRVGIYQVMPISEEIQRIILNSGNA
MEIAAQAKREGVNDLRTSGLIKVKQGLTTIEEVLGVTNE

Nucleotide


Download         Length: 1800 bp        

>NTDB_id=1122465 EL249_RS04285 WP_005674124.1 1055510..1057309(-) (pilB) [Lautropia mirabilis strain NCTC12852]
ATGGATCCTTCCCGGCTTTTCCTGATCCATGAGGGTAGGTTGCCCGTGTCCGCAAATCTTCCACCGCAAGCAAAGAACGA
ACGTGCCGACAAGGCCGATGCCGTGCAGCTGCCCGGCATTGCCCGCGTGCTGATCCAGCACCAGCTGCTGTCGCCCGAGC
AGGCGCGGGAAGCCCATGCCAAGGCTCAGGCAGCCGAGAACGGCAACTTCCTCGACGAGCTGGTCATCAACAGCAAGGTG
CTGAACCCGCTGCGGCTGGCCACCTTCCTGGCCGAGACCTTCGGCATGCCGCTGATGGATCTGGCCAGCCTGGACTTCAG
CACCCTGCCCCTGGACCAGATCGACCGCAAGCTGCTGGCCGAGACCCGCGTCATCCCGCTGATCAAGCGCGGCAACCGCC
TGGCCGTGCTGCTGTCCGACCCGACTGACAACCAGGCCATCGAGCGCGTCAAGTTCCAGCAGCAGGCCATCATCGACCCC
ATCGTCGTCGAGCACTCCAAGCTGGTCAAGCTGATCCAGAACCTGGACCAGAGCACCGCCGACAAGCTGGCCGACCTGAT
CGGTGACGTGGAAGAAGAAGAGTCCGCTGTCGCCGATGGCGCCGTGGCGCTGGAAGACAACGCCGACGTGGACGACGCGC
CGATCGTGCGCTTCCTGCAGAAGGTGCTGATGGATGCGATCCAGGGCGGTGCCTCGGACATCCACTTCGAGCCCTACGAG
AAGTTCTACCGGATCCGCTTCCGGGTGGACGGCGAGCTGCGTGAGATCACGCAGCCGCCCCTGGTCATCAAGGACAAGCT
CGCCTCGCGCATCAAGGTCATCTCCCGGCTGGACATCTCGGAGAAGCGGGTGCCGCAGGACGGCCGGATGAAGCTGGTCA
TCTCCAACAACCGCGCCATCGACTTCCGGGTCTCCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATGCGTATTCTG
GACCCCTCTTCCGCGAAGCTGGGGATCGATGCGCTGGGCTATGACCCCGAGCAGAAGAAGGCGCTGACCGAGGCCATCGC
CCGTCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTGTCGCTCTACACCTGCCTGAACATCC
TGAACCAGCCGGGCGTCAACATCGCCACCGCCGAGGACCCGGCCGAGATCAACCTGCCCGGCATCAACCAGGTCAACGTC
AACGACAAGGCAGGCCTGACCTTCGCTGCAGCCCTGAAATCCTTCCTGCGTCAGGATCCGGACATCATCATGGTGGGTGA
GATCCGCGACCTGGAAACTGCCGACATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGATGTCCACGCTGCACACCA
ACGACGCCCCCACCACGCTCACCCGTCTGATGAACATGGGCGTGGCACCCTTCAACATCGCCTCGTCGGTGATCCTGATC
ACCGCGCAGCGTCTGGCCCGTCGGCTGTGCACCCAGTGCAAGCAGCCCGTGGACCTGGACGATGACGCCCTGCTGGCCGC
CGGCTTCACCGAAGACGACCTGGACGGCAGCTGGCGCCCCTACAAGGCCGTGGGCTGCGAGCGCTGCAACGGCTCGGGCT
ACAAGGGCCGGGTGGGCATCTACCAGGTCATGCCGATCTCCGAAGAGATCCAGCGCATCATCCTCAATTCCGGCAACGCC
ATGGAAATTGCCGCCCAGGCCAAGCGCGAGGGCGTCAACGACCTGCGCACCTCGGGGCTGATCAAGGTCAAGCAAGGCCT
GACCACCATCGAGGAAGTGCTGGGCGTCACCAACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(221-488)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

94.825

0.534
  pilF Neisseria gonorrhoeae MS11

55.95

93.99

0.526
  pilB Acinetobacter baylyi ADP1

54.577

94.825

0.518
  pilB Legionella pneumophila strain ERS1305867

52.373

94.992

0.497
  pilB Vibrio cholerae strain A1552

47.237

93.656

0.442
  pilB Vibrio parahaemolyticus RIMD 2210633

45.341

93.155

0.422
  pilB Vibrio campbellii strain DS40M4

44.385

93.656

0.416
  pilF Thermus thermophilus HB27

39.547

95.826

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.292

94.324

0.371

Multiple sequence alignment