Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EL249_RS04275 Genome accession   NZ_LR134378
Coordinates   1053053..1053970 (-) Length   305 a.a.
NCBI ID   WP_005674126.1    Uniprot ID   E7RYM4
Organism   Lautropia mirabilis strain NCTC12852     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048053..1058970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL249_RS04260 (NCTC12852_00846) - 1051040..1051261 (-) 222 WP_005674129.1 DNA gyrase inhibitor YacG -
  EL249_RS04265 (NCTC12852_00847) zapD 1051261..1052016 (-) 756 WP_005674128.1 cell division protein ZapD -
  EL249_RS04270 (NCTC12852_00848) coaE 1052212..1052901 (-) 690 WP_005674127.1 dephospho-CoA kinase -
  EL249_RS04275 (NCTC12852_00849) pilD 1053053..1053970 (-) 918 WP_005674126.1 prepilin peptidase Machinery gene
  EL249_RS04280 (NCTC12852_00850) pilC 1054182..1055429 (-) 1248 WP_005674125.1 type II secretion system F family protein Machinery gene
  EL249_RS04285 (NCTC12852_00851) pilB 1055510..1057309 (-) 1800 WP_005674124.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL249_RS04290 (NCTC12852_00852) - 1057486..1058454 (-) 969 WP_040531668.1 Dyp-type peroxidase -

Sequence

Protein


Download         Length: 305 a.a.        Molecular weight: 32745.94 Da        Isoelectric Point: 6.4774

>NTDB_id=1122463 EL249_RS04275 WP_005674126.1 1053053..1053970(-) (pilD) [Lautropia mirabilis strain NCTC12852]
MGIEDIYWITPEFSVIVAGVVGLLVGSFLNVVIHRLPLMMQAQWEAELEEALAQQNPAAAEAAPPVEKKAPFNLLVPRSR
CPHCGHQITALENIPVLSWLFLRGACRECRKPIPVRYPLVELLTGVLAAASVWHLGFGVTGIAAAIFCCVLVALTFIDYD
TQLLPDSLTLPLLWGGLLLNLTSHGMAPLPDAVIGAMTGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWFGWQALP
AIILMSSVIGAVVGISLIFFKGHGRSQPIPFGPYLAGAGLAMLFLGGQVMTWMGVGVQPSVIIGP

Nucleotide


Download         Length: 918 bp        

>NTDB_id=1122463 EL249_RS04275 WP_005674126.1 1053053..1053970(-) (pilD) [Lautropia mirabilis strain NCTC12852]
ATGGGCATCGAAGACATCTACTGGATCACGCCGGAGTTCTCCGTGATCGTCGCCGGCGTGGTGGGCCTGCTGGTGGGCAG
CTTCCTCAACGTGGTCATCCACCGGCTGCCGCTGATGATGCAGGCCCAGTGGGAAGCCGAACTGGAAGAAGCGCTGGCAC
AACAGAACCCCGCTGCCGCAGAAGCTGCCCCCCCGGTCGAGAAGAAGGCCCCCTTCAACCTGCTGGTACCGCGCTCGCGC
TGCCCGCACTGCGGCCACCAGATCACCGCCCTGGAAAACATCCCGGTGCTGTCGTGGCTCTTCCTGCGCGGAGCCTGCCG
CGAATGCCGCAAGCCCATTCCGGTGCGCTACCCGCTGGTGGAACTGCTGACCGGCGTGCTGGCCGCCGCCAGCGTCTGGC
ATCTGGGCTTCGGCGTCACCGGCATTGCCGCCGCCATCTTCTGCTGCGTGCTGGTGGCCCTCACCTTTATCGACTACGAC
ACCCAGCTGCTGCCCGACAGCCTGACGCTGCCGCTGCTGTGGGGCGGGCTGCTGCTCAACCTTACCTCACACGGCATGGC
GCCGCTGCCCGATGCCGTCATCGGCGCCATGACCGGCTACCTGTCGCTGTGGAGCGTCTACTGGCTCTTCAAGCTGCTCA
CCGGCAAGGAAGGCATGGGCTACGGCGACTTCAAGCTGCTGGCCGCCCTGGGCGCGTGGTTCGGCTGGCAGGCCCTGCCC
GCCATCATCCTGATGTCCTCGGTCATCGGCGCCGTCGTGGGCATCAGCCTCATCTTCTTCAAGGGCCACGGCCGCAGCCA
GCCCATCCCCTTCGGCCCCTACCTGGCCGGCGCAGGCCTGGCCATGCTGTTCCTGGGTGGCCAGGTCATGACCTGGATGG
GAGTGGGTGTGCAGCCTTCGGTCATCATCGGGCCTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-134)

Type IV leader peptidase family (Peptidase_A24)

(146-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E7RYM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.778

94.426

0.498
  pilD Vibrio campbellii strain DS40M4

52.083

94.426

0.492
  pilD Neisseria gonorrhoeae MS11

48.014

90.82

0.436
  pilD Acinetobacter baumannii D1279779

46.377

90.492

0.42
  pilD Acinetobacter nosocomialis M2

46.182

90.164

0.416

Multiple sequence alignment