Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL255_RS18775 Genome accession   NZ_LR134376
Coordinates   4053923..4055629 (+) Length   568 a.a.
NCBI ID   WP_042653207.1    Uniprot ID   -
Organism   Aeromonas encheleia strain NCTC12917     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4048923..4060629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL255_RS18745 (NCTC12917_03763) pdhR 4050093..4050857 (-) 765 WP_042653203.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  EL255_RS18750 (NCTC12917_03764) ampD 4051134..4051706 (-) 573 WP_042653321.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL255_RS18755 (NCTC12917_03765) - 4051841..4052311 (+) 471 WP_042653204.1 TIGR02281 family clan AA aspartic protease -
  EL255_RS18760 (NCTC12917_03766) nadC 4052315..4053172 (+) 858 WP_042653205.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EL255_RS18770 - 4053494..4053919 (+) 426 WP_042653206.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EL255_RS18775 (NCTC12917_03767) pilB 4053923..4055629 (+) 1707 WP_042653207.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  EL255_RS18780 (NCTC12917_03768) pilC 4055760..4057001 (+) 1242 WP_042653208.1 type II secretion system F family protein Machinery gene
  EL255_RS18785 (NCTC12917_03769) pilD 4057106..4057978 (+) 873 WP_042653209.1 A24 family peptidase Machinery gene
  EL255_RS18790 (NCTC12917_03770) coaE 4057997..4058611 (+) 615 WP_042653210.1 dephospho-CoA kinase -
  EL255_RS18795 (NCTC12917_03771) zapD 4058649..4059371 (+) 723 WP_042653211.1 cell division protein ZapD -
  EL255_RS18800 (NCTC12917_03772) yacG 4059388..4059582 (+) 195 WP_042653212.1 DNA gyrase inhibitor YacG -
  EL255_RS18805 (NCTC12917_03773) mutT 4059639..4060046 (-) 408 WP_042653213.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62645.11 Da        Isoelectric Point: 5.8493

>NTDB_id=1122446 EL255_RS18775 WP_042653207.1 4053923..4055629(+) (pilB) [Aeromonas encheleia strain NCTC12917]
MTSSPNSGLATSLAASSLLSEPDSLRFLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTTAIGKLMENEQDALGMDHIDESEI
SELEVSDEGSRLDEGVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLRLSRHRSMDMRVNTLPTMWGEKIVIRLLDSSATRLNIEQLGFDDRQKAQYLKALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTNEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKTPEVVPDAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRNLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1122446 EL255_RS18775 WP_042653207.1 4053923..4055629(+) (pilB) [Aeromonas encheleia strain NCTC12917]
ATGACCTCTAGCCCCAATAGCGGCCTGGCCACAAGCCTGGCCGCCTCTTCCCTCCTCAGTGAGCCTGACTCACTACGCTT
CTTGAGCCAGGCCAGGGCGCAGCGCAAGCCTTTTGTCACCTTCCTGATCGAGAACGATATCCTCGACAGCAAGGCATTGG
CGGACTTCTGTGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACGGAAGCCTTGCTGGTCGAGGAGAGCA
AACTGACCACCGCCATCGGCAAGCTGATGGAGAACGAGCAGGATGCGCTCGGCATGGACCATATCGACGAGTCTGAGATC
TCGGAACTCGAAGTCTCTGACGAAGGCTCTCGGCTCGATGAGGGCGTCAATACGGCGGATGATGATGCCCCCATAGTGAA
GTACATCAACAAGATAATGATGGACGCCATCAAGCGCGGCGCCTCGGATCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTTCGGATCGACGGCATTCTGCATGAAATCGCCACCCCACCGGTCAACCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGGCGCCTGCCTCAGGATGGCCGGATCAAGTTGAGGCTGTCACGCCA
CAGATCTATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGTGAAAAGATTGTGATCCGCCTGCTGGACTCCTCGG
CGACTCGCCTCAACATAGAGCAACTCGGTTTCGACGACCGGCAGAAGGCCCAATACCTGAAGGCCCTCTCCAAGCCTCAG
GGCATGATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACGGTCTCACTCTATACCGGCCTCAACATTCTCAATACCAA
CGAGGTCAATATCTCTACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTTAACCAGGTGCAGATCAACCCCAAGG
CCGGACTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCGGATATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAGACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGTCATCTGGTGCTCTCGACCCTGCACACCAATTCGGCGGC
CGAGACCCTCACACGGATGATGAACATGGGGGTCCCCGCCTTCAACATCGCCTCCTCGGTGACTCTCATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGACACCGGAGGTGGTGCCGGACGCCGAGCTGCTGGAACTGGGCTTTACC
CCCCAGCAGCTGGCCGCCGGATTGCGGCTGTTCAAGCCGGTCGGTTGCAAGGAGTGCTCGGGAGGATACCGAGGCCGCGT
CGGCATCTATGAGATCATGCTCATGTCCGACAACATCGCGAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATAGCCG
CCATTGCCCAGAAGGAGGGCATGCGTAATCTGCGTATCTCGGGTCTCGAGAAGGCACGGTTGGGGGTCACCAGCCTGGCC
GAGATCAACCGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.386

100

0.546
  pilB Acinetobacter baumannii D1279779

56.377

95.246

0.537
  pilB Legionella pneumophila strain ERS1305867

53.982

99.472

0.537
  pilB Vibrio cholerae strain A1552

53.108

99.12

0.526
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.296

0.496
  pilF Neisseria gonorrhoeae MS11

45.567

99.296

0.452
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37

Multiple sequence alignment