Detailed information NTG1121841

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: EL140_RS03595
• Genome accession: NZ_LR134336
• Coordinates: 706816..707466 (+)
• Length: 216 a.a.
• NCBI ID: WP_000452010.1
• Uniprot ID: A0A7H9FBM2
• Organism: Streptococcus oralis ATCC 35037 strain NCTC 11427
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 701816..712466

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL140_RS03560 (NCTC11427_00700)	cvfB	702090..702944 (+)	855	WP_001095468.1	RNA-binding virulence regulatory protein CvfB	-
EL140_RS03565 (NCTC11427_00701)	-	702956..703561 (+)	606	WP_000727655.1	GrpB family protein	-
EL140_RS03570 (NCTC11427_00702)	-	703558..703773 (+)	216	WP_001232091.1	YozE family protein	-
EL140_RS03575 (NCTC11427_00703)	-	703854..704840 (+)	987	WP_000658168.1	PhoH family protein	-
EL140_RS03580 (NCTC11427_00704)	ald	704891..706003 (-)	1113	WP_000904713.1	alanine dehydrogenase	-
EL140_RS03590 (NCTC11427_00705)	-	706180..706749 (+)	570	WP_000443753.1	GNAT family N-acetyltransferase	-
EL140_RS03595 (NCTC11427_00706)	comEA/celA/cilE	706816..707466 (+)	651	WP_000452010.1	helix-hairpin-helix domain-containing protein	Machinery gene
EL140_RS03600 (NCTC11427_00707)	comEC/celB	707450..709690 (+)	2241	WP_000083028.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EL140_RS03605	infC	709910..710440 (+)	531	WP_000848184.1	translation initiation factor IF-3	-
EL140_RS03610 (NCTC11427_00709)	rpmI	710473..710673 (+)	201	WP_001125942.1	50S ribosomal protein L35	-
EL140_RS03615 (NCTC11427_00710)	rplT	710725..711084 (+)	360	WP_000124830.1	50S ribosomal protein L20	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23183.41 Da
• Isoelectric Point: 5.9255

>NTDB_id=1121841 EL140_RS03595 WP_000452010.1 706816..707466(+) (comEA/celA/cilE) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
MEVIIEKIKEYKIIVICAVLGLALGAFFLLKPTSQTSVKETNLQAEVAAVSKDSSSEKEIKKEEKEESPEQDLITVDVKG
AVKSPGIYDLPVGSRVHDAVQKAGGLTDEADSKSLNLAQKVSDEALVYVPTKGEEAASQQAASGTTPSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTAD

Nucleotide

• Length: 651 bp

>NTDB_id=1121841 EL140_RS03595 WP_000452010.1 706816..707466(+) (comEA/celA/cilE) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
ATGGAAGTAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTCATCTGTGCTGTTTTAGGCTTGGCCTTGGGCGCATT
TTTCCTACTAAAGCCAACTTCACAAACATCTGTGAAAGAAACAAACTTGCAGGCTGAAGTTGCAGCTGTTTCAAAGGATT
CATCATCTGAAAAGGAAATCAAGAAGGAAGAAAAGGAAGAATCTCCTGAACAAGATCTAATCACAGTAGATGTTAAGGGG
GCTGTTAAATCGCCAGGGATTTATGATTTGCCAGTAGGGAGTCGTGTTCATGATGCCGTTCAGAAGGCAGGTGGCTTGAC
AGATGAGGCAGATAGCAAGTCGCTCAATCTAGCACAGAAAGTCAGTGACGAGGCTCTTGTCTATGTTCCAACTAAGGGAG
AAGAAGCAGCTAGTCAGCAGGCTGCCTCTGGAACGACTCCTTCGACAAGTAAAGAAAAGAAAGTCAACCTCAATAAAGCT
AGTCTGGAAGAACTAAAGCAGGTCAAAGGCTTGGGAGGAAAACGAGCCCAGGATATTATCGACCATCGTGAGGCAAATGG
CAAATTCAAGTCGGTTGATGAATTAAAGAAGGTCTCTGGTATTGGCGCTAAGACCATAGAAAAGCTAAAAGATTATGTCA
CAGCGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A7H9FBM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	89.815	100	0.898
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	89.352	100	0.894
comEA/celA/cilE	Streptococcus pneumoniae D39	87.5	100	0.875
comEA/celA/cilE	Streptococcus pneumoniae R6	87.5	100	0.875
comEA/celA/cilE	Streptococcus pneumoniae Rx1	87.5	100	0.875
comEA/celA/cilE	Streptococcus mitis SK321	85.648	100	0.856
comEA	Lactococcus lactis subsp. cremoris KW2	41.485	100	0.44
comEA	Streptococcus thermophilus LMD-9	39.32	95.37	0.375