Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EL096_RS07570 Genome accession   NZ_LR134294
Coordinates   1402562..1403578 (-) Length   338 a.a.
NCBI ID   WP_114880773.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NCTC12977     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1397562..1408578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL096_RS12050 - 1397639..1397770 (-) 132 WP_000768904.1 hypothetical protein -
  EL096_RS07530 (NCTC12977_01466) - 1397771..1398961 (-) 1191 WP_000167766.1 acetate kinase -
  EL096_RS07535 (NCTC12977_01467) comYH 1399012..1399965 (-) 954 WP_050308921.1 class I SAM-dependent methyltransferase Machinery gene
  EL096_RS07540 - 1400026..1400620 (-) 595 Protein_1404 class I SAM-dependent methyltransferase -
  EL096_RS07545 (NCTC12977_01470) comGG/cglG 1400757..1401170 (-) 414 WP_050102600.1 competence type IV pilus minor pilin ComGG Machinery gene
  EL096_RS07550 (NCTC12977_01471) comGF/cglF 1401148..1401609 (-) 462 WP_000250544.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL096_RS07555 (NCTC12977_01472) comGE/cglE 1401572..1401874 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL096_RS07560 (NCTC12977_01473) comGD/cglD 1401837..1402241 (-) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL096_RS07565 (NCTC12977_01474) comGC/cglC 1402234..1402560 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  EL096_RS07570 (NCTC12977_01475) comGB/cglB 1402562..1403578 (-) 1017 WP_114880773.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL096_RS07575 (NCTC12977_01476) comGA/cglA/cilD 1403526..1404467 (-) 942 WP_000249549.1 competence type IV pilus ATPase ComGA Machinery gene
  EL096_RS07580 (NCTC12977_01477) - 1404543..1404908 (-) 366 WP_000286415.1 DUF1033 family protein -
  EL096_RS07585 (NCTC12977_01478) - 1405059..1406117 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EL096_RS07590 (NCTC12977_01479) nagA 1406280..1407431 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38548.65 Da        Isoelectric Point: 9.8287

>NTDB_id=1120573 EL096_RS07570 WP_114880773.1 1402562..1403578(-) (comGB/cglB) [Streptococcus pneumoniae strain NCTC12977]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETITFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVRTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1120573 EL096_RS07570 WP_114880773.1 1402562..1403578(-) (comGB/cglB) [Streptococcus pneumoniae strain NCTC12977]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCACCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCGGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae D39

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae R6

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae TIGR4

97.337

100

0.973
  comGB/cglB Streptococcus mitis SK321

94.083

100

0.941
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment