Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   EL087_RS04520 Genome accession   NZ_LR134288
Coordinates   921633..922475 (-) Length   280 a.a.
NCBI ID   WP_126407101.1    Uniprot ID   A0A4U9YZR9
Organism   Streptococcus milleri strain NCTC11169     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 916633..927475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL087_RS04505 (NCTC11169_00896) trmFO 917384..918718 (-) 1335 WP_126407097.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  EL087_RS04510 (NCTC11169_00897) - 918893..919390 (-) 498 WP_126407099.1 phosphoribosylanthranilate isomerase -
  EL087_RS04515 (NCTC11169_00898) topA 919454..921547 (-) 2094 WP_106083555.1 type I DNA topoisomerase -
  EL087_RS04520 (NCTC11169_00899) dprA/cilB/dalA 921633..922475 (-) 843 WP_126407101.1 DNA-processing protein DprA Machinery gene
  EL087_RS04525 (NCTC11169_00900) - 922558..923115 (-) 558 WP_126407103.1 sugar O-acetyltransferase -
  EL087_RS04530 (NCTC11169_00901) - 923105..923932 (-) 828 Protein_846 PRD domain-containing protein -
  EL087_RS04535 (NCTC11169_00902) - 923929..924711 (-) 783 WP_003029676.1 ribonuclease HII -
  EL087_RS04540 (NCTC11169_00903) ylqF 924698..925549 (-) 852 WP_003029675.1 ribosome biogenesis GTPase YlqF -
  EL087_RS04545 (NCTC11169_00904) - 925669..926586 (-) 918 WP_003029674.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 31086.57 Da        Isoelectric Point: 5.0636

>NTDB_id=1120264 EL087_RS04520 WP_126407101.1 921633..922475(-) (dprA/cilB/dalA) [Streptococcus milleri strain NCTC11169]
MDNFDIYKLKEAGLTNQQVINVLSYAEIQEKELSVKDMAVVSECRNPALFIEKYLQLDDDLLCQEFEKFPSFSILDDVYP
WDLSEIYNPPVLLFYKGNLDLLKLPKVAVVGSRNSSRTGSQSVQKIVKELNNELVIVSGLARGIDTSAHMAALQNGGRTI
AVIGTGLDVFYPRANKKLQSYLGEHHLVLSEYGPESEPLKFHFPERNRIIAGLCRGVIVAEAKMRSGSLITCERAMEEGR
DVFAIPGSILDGRSDGCHHLIQEGAKCVTSGADVLSEFQF

Nucleotide


Download         Length: 843 bp        

>NTDB_id=1120264 EL087_RS04520 WP_126407101.1 921633..922475(-) (dprA/cilB/dalA) [Streptococcus milleri strain NCTC11169]
ATGGATAATTTTGATATTTATAAATTAAAAGAAGCCGGCTTGACCAACCAACAGGTCATCAATGTTCTTTCGTATGCTGA
GATACAAGAAAAAGAATTATCAGTAAAAGATATGGCAGTTGTGTCGGAATGCCGTAATCCAGCTCTTTTCATTGAAAAAT
ATCTGCAGCTAGATGATGATTTATTATGTCAAGAATTTGAAAAATTTCCGTCTTTTTCTATCTTGGATGATGTGTATCCT
TGGGATTTGAGTGAAATTTACAATCCACCCGTTCTCCTTTTCTACAAAGGAAACTTGGATTTGTTAAAATTACCGAAAGT
AGCTGTTGTGGGAAGTCGCAATAGTAGCCGAACAGGCAGTCAATCTGTTCAAAAAATTGTTAAAGAGCTAAACAATGAGC
TAGTTATCGTAAGCGGCTTAGCGCGCGGAATTGATACATCAGCTCACATGGCAGCGTTGCAAAATGGTGGTCGAACGATT
GCCGTTATTGGAACCGGTTTAGATGTTTTTTATCCAAGAGCAAATAAAAAACTTCAATCTTATTTGGGAGAACATCACCT
TGTGCTTAGTGAATACGGTCCCGAATCAGAGCCATTGAAATTTCATTTTCCAGAGCGGAATCGCATTATTGCAGGTTTGT
GTAGAGGCGTTATTGTAGCTGAAGCCAAAATGCGCTCAGGAAGTCTAATTACTTGTGAGCGCGCAATGGAAGAAGGGCGA
GATGTGTTTGCTATTCCAGGGTCAATTTTAGACGGCAGAAGTGATGGCTGTCATCATTTGATTCAAGAGGGTGCAAAGTG
CGTCACATCAGGCGCTGACGTACTTTCTGAATTCCAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-269)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-60)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U9YZR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

76.786

100

0.768
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

76.429

100

0.764
  dprA/cilB/dalA Streptococcus pneumoniae D39

76.429

100

0.764
  dprA/cilB/dalA Streptococcus pneumoniae R6

76.429

100

0.764
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

76.429

100

0.764
  dprA/cilB/dalA Streptococcus mitis SK321

76.071

100

0.761
  dprA Streptococcus mutans UA159

65

100

0.65
  dprA Lactococcus lactis subsp. cremoris KW2

57.5

100

0.575
  dprA Staphylococcus aureus N315

37.943

100

0.382
  dprA Staphylococcus aureus MW2

37.722

100

0.379
  dprA Legionella pneumophila strain ERS1305867

51.232

72.5

0.371
  dprA Bacillus subtilis subsp. subtilis str. 168

45.374

81.071

0.368

Multiple sequence alignment