Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LMI_RS05490 Genome accession   NZ_LN614830
Coordinates   1228742..1230241 (+) Length   499 a.a.
NCBI ID   WP_052679464.1    Uniprot ID   A0A098GEV0
Organism   Legionella micdadei strain ATCC 33218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1223742..1235241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMI_RS05465 (LMI_1177) - 1224772..1225743 (-) 972 WP_045098894.1 hypothetical protein -
  LMI_RS05470 (LMI_1178) pilM 1226085..1227056 (+) 972 WP_161939854.1 type IV pilus assembly protein PilM -
  LMI_RS05475 (LMI_1180) - 1227046..1227591 (+) 546 WP_045098896.1 PilN domain-containing protein -
  LMI_RS05480 (LMI_1181) pilO 1227720..1228196 (+) 477 WP_161939853.1 type 4a pilus biogenesis protein PilO -
  LMI_RS05485 (LMI_1182) - 1228197..1228709 (+) 513 WP_045098898.1 pilus assembly protein PilP -
  LMI_RS05490 (LMI_1183) pilQ 1228742..1230241 (+) 1500 WP_052679464.1 type IV pilus secretin PilQ Machinery gene
  LMI_RS05495 (LMI_1184) aroK 1230543..1231070 (+) 528 WP_045098899.1 shikimate kinase AroK -
  LMI_RS05500 (LMI_1185) aroB 1231057..1232163 (+) 1107 WP_045098900.1 3-dehydroquinate synthase -
  LMI_RS05505 (LMI_1186) - 1232165..1233628 (+) 1464 WP_045098901.1 AAA family ATPase -
  LMI_RS05510 (LMI_1187) pnuC 1233833..1234399 (-) 567 WP_045098902.1 nicotinamide riboside transporter PnuC -
  LMI_RS05515 (LMI_1188) - 1234774..1235211 (+) 438 WP_045098903.1 universal stress protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 54912.94 Da        Isoelectric Point: 9.9615

>NTDB_id=1113646 LMI_RS05490 WP_052679464.1 1228742..1230241(+) (pilQ) [Legionella micdadei strain ATCC 33218]
MSRIILILILIIGNDCLFAMDSNVNGTKLSRVNQENKQEIVQTLPMEQKSYNNKDMVKFISLNFQEIKVSAVLQLLADFT
GINIVVSDKVTGSLSLRLDDILWDQALDIILTTRSLSKRKIGNVMFVAPVDEIIELEKKEFSMENDRRNFAPLRSDLLQV
NYAKAADIANLLTDPKTTLLSPRGSVNVDARTNTIWLQDIGTHVKKIRELVKRLDVPVKQVLIEARIVNATKDFARDLGV
RFGVSRPNHLVGRLKGIKQSVPYSGMEEEVPLAERLNLDLAALPITASPASVGVALAKLGNGILLDLELSALESEGKGEI
IANPRLITTNQQTAVIESGEEIPYQEATLSGSTAVAFKKAVLSLKVTPQITPDSKILMDLQINQNIPSVKVFNGVPSILT
KEIQTSVLINDGQTVVLGGIYKRDKYNALNRVPFLGELPVVGRLFRNKTTAVKNEELLIFITPRIITNNLSAVTMEGRRQ
AIAGGVKLGKFGKRVGSIH

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1113646 LMI_RS05490 WP_052679464.1 1228742..1230241(+) (pilQ) [Legionella micdadei strain ATCC 33218]
TTGAGTAGGATTATTCTAATTCTTATATTGATAATAGGAAATGATTGTTTGTTTGCAATGGATAGTAACGTAAATGGGAC
TAAATTGAGTCGCGTTAATCAGGAGAATAAACAGGAAATAGTCCAGACGCTACCTATGGAGCAGAAATCCTATAATAATA
AGGATATGGTCAAGTTTATTTCTTTAAATTTTCAGGAGATTAAAGTAAGCGCTGTCCTGCAATTATTGGCTGATTTTACT
GGAATAAATATAGTAGTCAGTGATAAAGTAACAGGTAGCTTAAGTTTGCGTTTGGATGACATACTTTGGGACCAAGCATT
GGATATTATTCTTACAACTCGATCTTTGAGTAAGCGAAAAATAGGCAATGTAATGTTTGTAGCTCCTGTTGATGAAATCA
TAGAGCTTGAAAAAAAAGAATTTAGTATGGAGAATGATCGCAGAAATTTTGCACCCCTGCGATCAGACTTACTACAAGTT
AATTATGCAAAAGCAGCTGATATTGCTAATTTACTAACAGACCCAAAAACTACACTATTATCTCCTCGAGGTAGTGTAAA
TGTGGATGCAAGAACCAACACCATTTGGTTACAGGATATTGGGACTCATGTTAAAAAAATAAGGGAGCTTGTAAAGCGCC
TTGATGTCCCTGTTAAACAAGTACTCATCGAGGCACGCATTGTCAATGCGACAAAAGATTTTGCAAGGGATTTGGGTGTC
CGTTTTGGTGTATCTAGGCCAAACCATTTAGTTGGCCGGTTAAAAGGCATTAAGCAGTCAGTACCATATAGTGGAATGGA
AGAAGAGGTCCCTTTGGCTGAACGTCTAAATCTTGATCTGGCTGCGCTACCAATTACAGCGTCGCCGGCATCAGTTGGCG
TTGCTTTAGCGAAATTGGGTAATGGTATACTTCTCGATTTAGAGCTTTCTGCACTTGAAAGTGAAGGAAAGGGAGAAATA
ATTGCTAATCCTAGATTAATAACAACCAATCAACAGACAGCTGTGATTGAGTCGGGAGAGGAAATACCATACCAAGAAGC
GACTTTGAGTGGGTCAACTGCTGTCGCATTCAAGAAAGCAGTACTAAGCTTAAAGGTGACACCACAAATTACACCAGATA
GTAAGATTTTGATGGATTTGCAGATTAATCAAAATATACCGTCTGTGAAGGTATTTAATGGAGTTCCTTCTATTTTAACA
AAGGAAATTCAGACAAGTGTCCTGATTAATGATGGACAAACGGTTGTTTTAGGGGGTATTTATAAAAGAGATAAGTACAA
TGCACTAAATCGTGTTCCTTTCCTAGGAGAATTGCCTGTCGTGGGACGATTATTTCGAAACAAAACGACTGCGGTTAAAA
ATGAGGAGTTGCTCATTTTCATTACTCCTAGGATAATAACAAACAACTTGTCAGCAGTAACGATGGAAGGGCGAAGGCAA
GCGATTGCAGGAGGTGTTAAGTTGGGCAAGTTTGGTAAGCGAGTAGGATCGATTCATTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(158-220)

Bacterial type II and III secretion system protein (Secretin)

(311-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098GEV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

63.974

91.784

0.587
  pilQ Legionella pneumophila strain Lp02

63.974

91.784

0.587
  comQ Acinetobacter baylyi ADP1

42.283

94.79

0.401
  pilQ Vibrio cholerae strain A1552

47.242

83.567

0.395
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

47.242

83.567

0.395
  pilQ Pseudomonas aeruginosa PAK

41.756

93.587

0.391
  pilQ Vibrio campbellii strain DS40M4

45.882

85.17

0.391
  pilQ Acinetobacter baumannii strain A118

40.87

92.184

0.377
  pilQ Acinetobacter baumannii D1279779

40.523

91.984

0.373

Multiple sequence alignment