Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BN1079_RS08830 Genome accession   NZ_CCSF01000001
Coordinates   1898434..1900137 (-) Length   567 a.a.
NCBI ID   WP_037023749.1    Uniprot ID   A0A078LTL0
Organism   Pseudomonas saudiphocaensis strain 20_BN     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1893434..1905137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN1079_RS08800 (BN1079_01804) - 1893595..1893846 (-) 252 WP_037023740.1 cytochrome C oxidase subunit IV family protein -
  BN1079_RS08805 (BN1079_01805) - 1893857..1894444 (-) 588 WP_037023742.1 cytochrome c oxidase subunit 3 -
  BN1079_RS08810 (BN1079_01806) - 1894428..1895207 (-) 780 WP_171819327.1 CbbQ/NirQ/NorQ/GpvN family protein -
  BN1079_RS08815 (BN1079_01807) - 1895773..1896087 (+) 315 WP_037023746.1 c-type cytochrome -
  BN1079_RS08820 (BN1079_01808) pilD 1896184..1897050 (-) 867 WP_037026723.1 prepilin peptidase Machinery gene
  BN1079_RS08825 (BN1079_01810) pilC 1897214..1898431 (-) 1218 WP_037023748.1 type II secretion system F family protein Machinery gene
  BN1079_RS08830 (BN1079_01811) pilB 1898434..1900137 (-) 1704 WP_037023749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BN1079_RS08835 (BN1079_01812) - 1900459..1900896 (+) 438 WP_037023750.1 pilin -
  BN1079_RS08840 (BN1079_01813) - 1901246..1902634 (+) 1389 WP_037023751.1 O-antigen ligase family protein -
  BN1079_RS08845 (BN1079_01814) - 1902684..1903328 (-) 645 WP_037023752.1 TIGR02281 family clan AA aspartic protease -
  BN1079_RS08850 (BN1079_01815) - 1903670..1904137 (-) 468 WP_139053075.1 phosphatidylglycerophosphatase A -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61966.97 Da        Isoelectric Point: 5.3441

>NTDB_id=111345 BN1079_RS08830 WP_037023749.1 1898434..1900137(-) (pilB) [Pseudomonas saudiphocaensis strain 20_BN]
MSDTISLSGLARQMVQAGVMDERTAQQAQVQALRSQTSLVTYVVQNKLAKGRAVAELASEQFGVVLFDLAALDKEVQPRE
IVSEKLARQHRALPLVKRGNKLYVAISDPSNQQAISDIQFSTGLTTEGILVEDDKLGVALDKFFEGGATGLEDMGDIDLD
GLDVETVSDDGKADDAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTFRVRLRTDGVLHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKNKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKKLYMDALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCSSCKKEVELPREALLHEGFP
EEKIGTFKLYGPVGCENCTGGYKGRVGIYEVVKNTPALARLIMEDGNSIDISTQMRKDGFNDLRTSALIKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=111345 BN1079_RS08830 WP_037023749.1 1898434..1900137(-) (pilB) [Pseudomonas saudiphocaensis strain 20_BN]
ATGAGCGACACTATTTCCCTATCCGGCCTAGCCCGGCAGATGGTGCAAGCCGGAGTGATGGATGAACGGACGGCCCAACA
GGCGCAGGTGCAGGCGCTGCGTAGTCAGACATCACTGGTCACCTATGTAGTACAGAACAAGTTGGCCAAGGGACGTGCGG
TCGCGGAGCTGGCCTCCGAGCAGTTTGGCGTGGTGCTGTTCGACCTGGCTGCGCTGGATAAGGAGGTTCAGCCCAGGGAG
ATCGTCAGCGAAAAGCTGGCGCGCCAGCATCGGGCGCTGCCATTGGTTAAACGTGGCAACAAACTATATGTAGCGATATC
AGACCCGAGTAATCAGCAGGCAATCAGTGATATCCAGTTCAGCACTGGGCTGACCACCGAAGGCATTCTGGTAGAAGACG
ACAAGCTGGGCGTGGCGCTGGACAAGTTCTTCGAGGGTGGCGCTACCGGTCTGGAAGACATGGGCGACATCGATCTGGAT
GGTCTGGATGTCGAGACGGTCAGCGATGACGGCAAAGCCGATGACGCTGGCGAGGCTGCCGACGATGCGCCAGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCCATACGTGGCGGCTCATCCGACCTGCATTTCGAACCCTACGAGAAAACCTTTC
GGGTGCGCCTGCGTACCGACGGGGTGTTGCATGAGGTGGCGCGGCCGCCGATCCAGCTGGCGCCACGGATTTCGGCGCGC
CTGAAAGTGATGGCCGGATTGGATATTTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGCTGTCGAAGAA
CAAGGCCATCGACTTTCGGGTTAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCTTCCA
GTGCGCAGATGGGCATCGATGCGTTGGGTTATGAAGAGTCGCAGAAAAAGCTCTATATGGATGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTGACGGGGCCAACCGGCTCGGGCAAGACGGTTTCCCTTTATACGGGGCTGAATATCCTCAACACGCC
GGATGTGAATATCTCCACGGCGGAAGACCCGGTAGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGGATGGACTTTGCGCAGGCGCTGCGCGCCTTTCTGCGTCAGGATCCGGACATCATCATGGTCGGCGAGATTCGTGAC
CTGGAAACTGCGGAGATTGCCATCAAGGCCGCGCAGACCGGCCATATGGTGATGTCGACGCTGCACACCAACAGCGCCGC
GGAAACCCTGACGCGTCTGCGCAACATGGGTGTGGCAGCCTTCAATATTGCCACCTCGGTGAACCTGATCATTGCTCAAC
GTCTGGCACGCAAGCTGTGCAGCAGTTGCAAGAAAGAGGTCGAGCTGCCGCGCGAGGCGCTGCTGCATGAAGGATTCCCG
GAAGAAAAAATCGGGACTTTCAAACTGTACGGGCCGGTAGGCTGTGAAAACTGTACAGGTGGTTACAAAGGGCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCTGCACTGGCCCGGCTTATCATGGAAGACGGTAACTCAATCGATATTTCCACCC
AGATGCGCAAGGACGGCTTTAACGACCTGCGGACTTCGGCGCTGATCAAGGCCATGCAGGGCGTGACGAGCCTGGAAGAA
GTCAACCGGGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A078LTL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.574

99.471

0.593
  pilB Acinetobacter baylyi ADP1

58.688

99.471

0.584
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.547
  pilF Neisseria gonorrhoeae MS11

49.558

99.647

0.494
  pilB Vibrio cholerae strain A1552

50

98.765

0.494
  pilB Vibrio campbellii strain DS40M4

47.909

100

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

51.816

92.24

0.478
  pilB/pilB1 Synechocystis sp. PCC 6803

36.731

100

0.4

Multiple sequence alignment