Detailed information NTG0111343

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BN1079_RS08820
• Genome accession: NZ_CCSF01000001
• Coordinates: 1896184..1897050 (-)
• Length: 288 a.a.
• NCBI ID: WP_037026723.1
• Uniprot ID: A0A078LX96
• Organism: Pseudomonas saudiphocaensis strain 20_BN
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1891184..1902050

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BN1079_RS08790 (BN1079_01801)	-	1891298..1892728 (-)	1431	WP_037023738.1	cbb3-type cytochrome c oxidase subunit I	-
BN1079_RS08795 (BN1079_01802)	-	1892732..1893172 (-)	441	WP_037023739.1	c-type cytochrome	-
BN1079_RS08800 (BN1079_01804)	-	1893595..1893846 (-)	252	WP_037023740.1	cytochrome C oxidase subunit IV family protein	-
BN1079_RS08805 (BN1079_01805)	-	1893857..1894444 (-)	588	WP_037023742.1	cytochrome c oxidase subunit 3	-
BN1079_RS08810 (BN1079_01806)	-	1894428..1895207 (-)	780	WP_171819327.1	CbbQ/NirQ/NorQ/GpvN family protein	-
BN1079_RS08815 (BN1079_01807)	-	1895773..1896087 (+)	315	WP_037023746.1	c-type cytochrome	-
BN1079_RS08820 (BN1079_01808)	pilD	1896184..1897050 (-)	867	WP_037026723.1	prepilin peptidase	Machinery gene
BN1079_RS08825 (BN1079_01810)	pilC	1897214..1898431 (-)	1218	WP_037023748.1	type II secretion system F family protein	Machinery gene
BN1079_RS08830 (BN1079_01811)	pilB	1898434..1900137 (-)	1704	WP_037023749.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BN1079_RS08835 (BN1079_01812)	-	1900459..1900896 (+)	438	WP_037023750.1	pilin	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 32008.10 Da
• Isoelectric Point: 8.2368

>NTDB_id=111343 BN1079_RS08820 WP_037026723.1 1896184..1897050(-) (pilD) [Pseudomonas saudiphocaensis strain 20_BN]
MIFEFLASNTLAFVLCVLVLGLLVGSFLNVLIYRLPVMMQRQWRAQAREFLELPVDSNGAPFNLLLPHSRCPHCNHRIRA
WENVPLASWLALRGKCSACKAPISKRYPLVELACGVLSGYIAWHFGFTWQAGAMLLLTWGLLAMSMIDVDHQLLPDSLVL
PLLWLGLIVNSFSLFASLEDALWGAVAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVGA
VLGTIMLRMQKAESSTPIPFGPYLAIAGWIALLWGEQLTASYLQFARF

Nucleotide

• Length: 867 bp

>NTDB_id=111343 BN1079_RS08820 WP_037026723.1 1896184..1897050(-) (pilD) [Pseudomonas saudiphocaensis strain 20_BN]
ATGATATTTGAGTTTCTGGCCAGCAATACGCTGGCCTTTGTTTTGTGTGTGCTGGTGCTGGGGTTGCTGGTGGGCAGCTT
TCTTAACGTCCTTATTTACCGTTTGCCGGTCATGATGCAGCGGCAGTGGCGTGCACAGGCGCGGGAGTTCCTGGAGTTGC
CGGTCGACTCGAATGGAGCGCCGTTCAATCTGCTTTTACCGCACTCTCGATGCCCGCATTGCAATCATCGGATTCGAGCC
TGGGAGAACGTTCCGCTGGCTAGCTGGCTGGCGCTGCGTGGCAAGTGCTCGGCATGCAAGGCGCCGATCAGCAAGCGCTA
TCCGCTAGTGGAGCTGGCGTGTGGAGTGCTCTCGGGATATATCGCCTGGCATTTTGGATTCACCTGGCAGGCAGGAGCGA
TGTTGCTGCTGACCTGGGGGTTGCTGGCGATGAGCATGATCGATGTCGATCACCAGCTGCTGCCGGACTCGCTGGTGCTG
CCGCTGCTATGGCTGGGGCTGATCGTTAACAGTTTCAGCCTGTTCGCTTCGCTGGAAGACGCGCTATGGGGGGCTGTGGC
GGGCTATCTCAGCCTGTGGTCGGTGTACTGGTTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGAGACTTCA
AGTTACTGGCCATGCTGGGCGCCTGGGGCGGTTGGCAGGTGCTTCCGCTGACCATATTGCTGTCTTCTGTGGTCGGCGCG
GTGCTGGGAACCATCATGCTGCGCATGCAGAAAGCGGAGAGCAGTACGCCTATTCCCTTTGGCCCCTATCTGGCGATTGC
CGGCTGGATTGCGTTGCTCTGGGGCGAGCAGCTCACTGCCAGTTATCTGCAGTTTGCCCGGTTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: A0A078LX96

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.143	94.792	0.542
pilD	Vibrio campbellii strain DS40M4	53.846	94.792	0.51
pilD	Acinetobacter nosocomialis M2	48.592	98.611	0.479
pilD	Acinetobacter baumannii D1279779	48.239	98.611	0.476
pilD	Neisseria gonorrhoeae MS11	52.964	87.847	0.465