Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KHN94_RS11560 Genome accession   NZ_HG992758
Coordinates   2564796..2566481 (+) Length   561 a.a.
NCBI ID   WP_182031853.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16 isolate B1REV17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2559796..2571481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN94_RS11540 (ACOMICROBIO_LOCUS1675) pdhR 2561149..2561916 (-) 768 WP_182031850.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  KHN94_RS11545 ampD 2562327..2562871 (-) 545 Protein_2257 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KHN94_RS11550 (ACOMICROBIO_LOCUS1678) nadC 2563243..2564130 (+) 888 WP_182031851.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KHN94_RS11555 (ACOMICROBIO_LOCUS1679) pilA 2564392..2564796 (+) 405 WP_182031852.1 pilin Machinery gene
  KHN94_RS11560 (ACOMICROBIO_LOCUS1680) pilB 2564796..2566481 (+) 1686 WP_182031853.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHN94_RS11565 (ACOMICROBIO_LOCUS1681) pilC 2566512..2567735 (+) 1224 WP_182031854.1 type II secretion system F family protein Machinery gene
  KHN94_RS11570 - 2567806..2568676 (+) 871 Protein_2262 A24 family peptidase -
  KHN94_RS11575 (ACOMICROBIO_LOCUS1683) coaE 2568677..2569291 (+) 615 WP_182031856.1 dephospho-CoA kinase -
  KHN94_RS11580 (ACOMICROBIO_LOCUS1684) zapD 2569319..2570059 (+) 741 WP_182031857.1 cell division protein ZapD -
  KHN94_RS11585 (ACOMICROBIO_EPCKBFOG_02371) yacG 2570164..2570358 (+) 195 WP_182031858.1 DNA gyrase inhibitor YacG -
  KHN94_RS11590 (ACOMICROBIO_LOCUS1685) rplS 2570729..2571082 (-) 354 WP_145532528.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61901.77 Da        Isoelectric Point: 5.7458

>NTDB_id=1112528 KHN94_RS11560 WP_182031853.1 2564796..2566481(+) (pilB) [Vibrio sp. B1FLJ16 isolate B1REV17]
MQTNLLTVLRQSGLLSLVQEESLIEQVNASGISMPEALVRSGYLTPEELTTHLSIAVGLPCVKLADYQYVSLCQQLGLRE
LITRHHALPLERTSSTLTLAVADPTNQQAEDDFRFATGFQVDLVIADFAEIDAAIRRLYGRSIGQEKPGLKEINQDELAS
LVDIGADEVDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSAASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKAAQTGHLVLSTLHTNSAAETVVRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQPQELTAALQHVGIHSTEN
IFQANPHGCNACTHGYSGRTGIYEVMHFNESLSEALIKGASVHELEKLAISHGMSTLQMSGLEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1112528 KHN94_RS11560 WP_182031853.1 2564796..2566481(+) (pilB) [Vibrio sp. B1FLJ16 isolate B1REV17]
GTGCAAACCAACCTCTTAACGGTTCTTCGCCAATCCGGGTTACTTAGCTTAGTCCAAGAAGAATCGTTAATAGAGCAAGT
CAACGCTTCTGGCATTTCGATGCCGGAAGCGTTAGTTCGTTCTGGCTATCTCACACCAGAAGAGCTGACTACGCACCTTA
GTATCGCAGTCGGGCTACCTTGCGTAAAACTCGCTGACTACCAATATGTATCTCTATGCCAACAACTTGGCTTGCGTGAA
TTGATCACCCGTCATCACGCACTGCCACTCGAGCGTACGTCCTCAACACTCACGTTGGCTGTCGCAGATCCGACCAATCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACCGGATTTCAGGTCGATCTAGTAATTGCTGACTTTGCCGAAATTGATGCCG
CTATCCGGCGATTGTATGGCCGCTCGATTGGTCAGGAAAAACCGGGCTTAAAAGAGATCAACCAAGACGAGCTGGCGAGC
CTTGTTGATATCGGTGCTGACGAAGTCGACAACATCGAAGATTTAAGCCAGGACGAATCTCCGGTCAGTCGCTATATCAA
CCAGATTTTATTGGACGCGGTGCGCAAAGGTGCATCGGATATCCACTTTGAACCGTATGAAAAAATGTACCGTGTGCGCC
TGCGCTGTGACGGTATCTTGATTGAAACCCAGCAACCTCCCAGCCACTTAAGCCGCCGCTTATCAGCGCGGATCAAAATA
CTGTCTAAACTGGATATCGCCGAAAGGCGCTTGCCCCAAGACGGGCGAATCAAGTTAAAACTCAATCAAGACACCGCGAT
TGATATGCGGGTATCGACCCTGCCTACTCTGTTTGGTGAAAAGATCGTACTGCGTCTGCTCGACAGCAGTGCTGCGTCAC
TGGATATCGATAAACTCGGTTACAGTGAGCAACAAAAACAGTTGTATCTCGATGCGTTGCGCCGTCCCCAAGGAATGATT
TTAATGACCGGCCCGACCGGGAGTGGTAAAACCGTCTCGCTATACACAGGGCTAAATATTCTCAATAAACCAGAAATCAA
TATCTCTACTGCGGAAGATCCGGTAGAAATCAACTTATCCGGCATTAATCAGGTTCAGGTTCAGCCTAAAATTGGCTTTG
GTTTTGCTGAGGCGCTGCGATCCTTCTTGCGTCAAGACCCGGATGTGGTGATGGTCGGGGAAATCCGTGACCTCGATACC
GCAGAAATCGCAATTAAAGCCGCACAAACCGGTCACTTAGTGCTTTCTACCCTGCATACCAACTCTGCTGCCGAAACCGT
GGTTCGTCTGTCTAATATGGGGGTGGAAAGCTTTAACCTCGCCTCTTCCCTAAGCCTTATCATTGCTCAACGCTTGGCAC
GAAAACTTTGCTCTCACTGCAAGCAGCCACAAGAACTTACCGCTGCTTTACAACATGTCGGTATTCACTCCACCGAAAAC
ATTTTTCAAGCTAACCCACACGGATGTAATGCATGCACTCACGGCTACTCAGGCCGTACCGGTATTTATGAAGTGATGCA
CTTTAATGAATCCTTATCAGAAGCACTGATTAAAGGCGCCTCTGTACACGAACTGGAAAAGCTCGCCATCAGTCATGGCA
TGAGCACCCTACAGATGTCCGGTTTAGAGAAACTAAAACAAGGGGTCACCAGCTTTAGTGAGCTCCAACGCGTGCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

87.522

100

0.875
  pilB Vibrio campbellii strain DS40M4

86.096

100

0.861
  pilB Vibrio cholerae strain A1552

72.776

100

0.729
  pilB Acinetobacter baumannii D1279779

50.566

94.474

0.478
  pilB Legionella pneumophila strain ERS1305867

49.165

96.078

0.472
  pilB Acinetobacter baylyi ADP1

49.336

93.939

0.463
  pilF Neisseria gonorrhoeae MS11

44.149

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

37.797

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

99.643

0.385
  pilF Thermus thermophilus HB27

37.205

98.217

0.365

Multiple sequence alignment