Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LOK40_RS14140 Genome accession   NZ_HG992336
Coordinates   3300495..3301625 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas arboricola strain 1311a isolate 1311a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3295495..3306625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOK40_RS14110 (XA1311A_27530) - 3295884..3296324 (-) 441 WP_016902361.1 OsmC family protein -
  LOK40_RS14115 (XA1311A_27540) - 3296542..3297489 (-) 948 WP_016902360.1 aspartate carbamoyltransferase catalytic subunit -
  LOK40_RS14120 (XA1311A_27550) ruvX 3297505..3297972 (-) 468 WP_024939388.1 Holliday junction resolvase RuvX -
  LOK40_RS14125 (XA1311A_27560) - 3297965..3298531 (-) 567 WP_011051924.1 YqgE/AlgH family protein -
  LOK40_RS14130 (XA1311A_27570) - 3298867..3299412 (+) 546 WP_228607729.1 DNA-3-methyladenine glycosylase I -
  LOK40_RS14135 (XA1311A_27580) - 3299539..3300213 (-) 675 WP_164739560.1 YitT family protein -
  LOK40_RS14140 (XA1311A_27590) pilU 3300495..3301625 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOK40_RS14145 (XA1311A_27600) pilT 3301748..3302785 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  LOK40_RS14150 (XA1311A_27610) - 3303108..3303800 (+) 693 WP_102584992.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOK40_RS14155 (XA1311A_27620) proC 3303862..3304707 (+) 846 WP_102584993.1 pyrroline-5-carboxylate reductase -
  LOK40_RS14160 (XA1311A_27630) - 3305306..3305731 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  LOK40_RS14165 (XA1311A_27640) - 3305816..3306487 (+) 672 WP_228607730.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1112294 LOK40_RS14140 WP_016902355.1 3300495..3301625(-) (pilU) [Xanthomonas arboricola strain 1311a isolate 1311a]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1112294 LOK40_RS14140 WP_016902355.1 3300495..3301625(-) (pilU) [Xanthomonas arboricola strain 1311a isolate 1311a]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGTCCGATCACCCAGACTCCGCTGACGGCGCAACAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTTCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACGGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTTGGCG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGACACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAATGCACTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCATGCCGTGGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCGAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGTTGATTCCGACCCCGGATGGCCGCGGCCGGCGCGTGGCGATGGAGATCATGCTGGGTACACCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment