Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   SPYH293_RS00645 Genome accession   NZ_HG316453
Coordinates   101868..102806 (+) Length   312 a.a.
NCBI ID   WP_020904831.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain H293     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96868..107806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYH293_RS00635 (SPYH293_00133) rpoC 97617..101258 (+) 3642 WP_031488660.1 DNA-directed RNA polymerase subunit beta' -
  SPYH293_RS00640 (SPYH293_00134) - 101410..101775 (+) 366 WP_002986560.1 DUF1033 family protein -
  SPYH293_RS00645 (SPYH293_00135) comYA 101868..102806 (+) 939 WP_020904831.1 competence type IV pilus ATPase ComGA Machinery gene
  SPYH293_RS00650 (SPYH293_00136) comYB 102742..103776 (+) 1035 WP_226316548.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPYH293_RS00655 (SPYH293_00137) comYC 103778..104104 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  SPYH293_RS00660 (SPYH293_00138) comGD 104079..104507 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  SPYH293_RS00665 (SPYH293_00139) comGE 104464..104748 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  SPYH293_RS00670 (SPYH293_00140) comYF 104741..105175 (+) 435 WP_020904833.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPYH293_RS00675 (SPYH293_00141) comGG 105159..105485 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  SPYH293_RS00680 (SPYH293_00142) comYH 105583..106536 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  SPYH293_RS00685 (SPYH293_00143) - 106595..107791 (+) 1197 WP_020904834.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35041.28 Da        Isoelectric Point: 8.5825

>NTDB_id=1111843 SPYH293_RS00645 WP_020904831.1 101868..102806(+) (comYA) [Streptococcus pyogenes strain H293]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGHESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1111843 SPYH293_RS00645 WP_020904831.1 101868..102806(+) (comYA) [Streptococcus pyogenes strain H293]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCATGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.359

0.603
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

60.129

99.679

0.599
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.679

0.593
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment