Detailed information NTG1111680

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HMPREF9319_RS02540
• Genome accession: NZ_GL397128
• Coordinates: 510722..511387 (+)
• Length: 221 a.a.
• NCBI ID: WP_003063831.1
• Uniprot ID: E0PCE3
• Organism: Streptococcus equinus ATCC 700338
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 505722..516387

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF9319_RS10635	-	505797..506975 (+)	1179	Protein_496	MFS transporter	-
HMPREF9319_RS02515 (HMPREF9319_0511)	-	507030..507890 (+)	861	WP_003063816.1	helix-turn-helix transcriptional regulator	-
HMPREF9319_RS02520 (HMPREF9319_0512)	-	507895..508524 (+)	630	WP_052292720.1	ROK family protein	-
HMPREF9319_RS02525 (HMPREF9319_0513)	-	508756..509037 (-)	282	WP_003063819.1	GIY-YIG nuclease family protein	-
HMPREF9319_RS02530 (HMPREF9319_0514)	-	509012..509773 (-)	762	WP_003063822.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
HMPREF9319_RS02535 (HMPREF9319_0515)	-	509881..510627 (+)	747	WP_003063827.1	lysophospholipid acyltransferase family protein	-
HMPREF9319_RS02540 (HMPREF9319_0516)	comEA	510722..511387 (+)	666	WP_003063831.1	helix-hairpin-helix domain-containing protein	Machinery gene
HMPREF9319_RS02545 (HMPREF9319_0517)	comEC/celB	511380..513611 (+)	2232	WP_041972902.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HMPREF9319_RS02550 (HMPREF9319_0518)	holA	513685..514722 (+)	1038	WP_003063837.1	DNA polymerase III subunit delta	-
HMPREF9319_RS02555 (HMPREF9319_0519)	sodA	514811..515419 (+)	609	WP_041972900.1	superoxide dismutase SodA	-
HMPREF9319_RS02560 (HMPREF9319_0520)	-	515568..516293 (+)	726	WP_003063840.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23603.32 Da
• Isoelectric Point: 4.2822

>NTDB_id=1111680 HMPREF9319_RS02540 WP_003063831.1 510722..511387(+) (comEA) [Streptococcus equinus ATCC 700338]
MIEEIKEKLLENKTLAAVLGIILVMIIGFFAWLNVTKTTADAQNDLPALSTTFSTSSSASDLKTERSSSQTETQKMFVDI
KGAVKNEGVYELSNGSRVTDVVKLAGGFTEDADKKSVNLAEKVTDESVIYVARVDENVAPATTNSQANGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=1111680 HMPREF9319_RS02540 WP_003063831.1 510722..511387(+) (comEA) [Streptococcus equinus ATCC 700338]
ATGATTGAAGAAATAAAAGAAAAACTTTTGGAGAACAAAACGTTGGCAGCGGTATTGGGGATAATACTTGTCATGATAAT
CGGATTTTTTGCTTGGTTAAATGTGACAAAAACAACAGCGGATGCGCAAAATGACCTACCTGCCCTAAGCACGACTTTTT
CGACAAGTAGTAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAGACTCAAAAAATGTTTGTAGATATC
AAAGGTGCTGTGAAAAATGAGGGGGTTTATGAGCTTTCAAACGGTAGCCGTGTGACAGATGTGGTCAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATCTGGCTGAAAAAGTAACCGATGAATCGGTGATTTATGTGGCAAGAG
TTGATGAAAATGTTGCTCCTGCAACGACTAATTCACAAGCAAATGGCAGCGCTCAACAGGAAGAAAGTTCAGATAAAATC
AACCTTAATACCGCAACTTTGGCGGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGATATTATTGACTA
CCGTGATGCTAATGGTGGTTTTTCATCAGTTGATGACTTAGCGAATGTGTCTGGTATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (157-219)

• SLBB domain (SLBB) (78-131)

3D structure

• AlphaFold DB: E0PCE3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.28	100	0.511
comEA/celA/cilE	Streptococcus mitis SK321	46.154	100	0.462
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.891	100	0.439
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae D39	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae R6	43.636	99.548	0.434
comEA	Latilactobacillus sakei subsp. sakei 23K	37.946	100	0.385