Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOI0R_RS07295 Genome accession   NZ_CP184802
Coordinates   391857..393548 (-) Length   563 a.a.
NCBI ID   WP_237029686.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. piscicida strain DQ-SS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386857..398548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOI0R_RS07265 mutT 387371..387811 (-) 441 WP_420745475.1 8-oxo-dGTP diphosphatase MutT -
  ACOI0R_RS07270 yacG 387878..388087 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  ACOI0R_RS07275 zapD 388095..388832 (-) 738 WP_420745476.1 cell division protein ZapD -
  ACOI0R_RS07280 coaE 388900..389502 (-) 603 WP_420745477.1 dephospho-CoA kinase -
  ACOI0R_RS07285 pilD 389504..390382 (-) 879 WP_065170821.1 prepilin peptidase Machinery gene
  ACOI0R_RS07290 pilC 390565..391791 (-) 1227 WP_341488890.1 type II secretion system F family protein Machinery gene
  ACOI0R_RS07295 pilB 391857..393548 (-) 1692 WP_237029686.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOI0R_RS07300 - 393548..394045 (-) 498 WP_420745478.1 pilin -
  ACOI0R_RS07305 ampD 394513..395091 (+) 579 WP_138222126.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACOI0R_RS07310 pdhR 395451..396224 (+) 774 WP_176285891.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62525.17 Da        Isoelectric Point: 5.4754

>NTDB_id=1111332 ACOI0R_RS07295 WP_237029686.1 391857..393548(-) (pilB) [Photobacterium damselae subsp. piscicida strain DQ-SS1]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLCQSANTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDEERDQATDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=1111332 ACOI0R_RS07295 WP_237029686.1 391857..393548(-) (pilB) [Photobacterium damselae subsp. piscicida strain DQ-SS1]
ATGCACACCACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATTCATCTGGGTATATTTAGCGATCGCGAACTGGCACAACACCTTA
GCCACATTTTTGGTCTCACCCTCGTTTCAGTGCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGTCATCGCGCACTACCGTTATGCCAAAGCGCCAATACGTTATTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGACAAGATGAGTTCCGTTTTGCCACTGGCCGCAATATTGAGCCGCTACTACTAGAAAACAAACAGCTCGAATCAG
CTATCCGTCGTATTTATGGTCGTGAGTTGGGCGAAACCACCAATATGGCCAGTAGGGATGTCAGCGACGATGATCTGAAT
AACTTAGTCGATATTGGTGATGAAGAGCGAGATCAAGCGACAGATCTAGGTCAAGAAAATGAACCTGTTACTCGCTATAT
CAACCAAGTGCTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCTTATGAGACCAGCTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTCTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCACAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTCCGTGTTTCTACCCTGCCCACCTTATGGGGCGAAAAAGTGGTGCTGCGTATTTTAGATAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTACCTTAATGCTCTGCAACAGCCGCAAGGAATG
ATCTTAATGACAGGACCGACGGGCAGCGGTAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATATCTACCGCTGAAGATCCGGTAGAGATCAACTTACCTGGCATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCTGATGTAGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATCGCCGTTAAAGCAGCGCAAACTGGACATTTAGTACTATCGACCTTGCACACCAATTCTGCTGCCGAAAC
CATCACCCGCTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTGGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCAGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGTCGTCGTCTTGCTCAAGCTCTGGTTGATGGCGCAACTACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGTATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.634

100

0.616
  pilB Vibrio parahaemolyticus RIMD 2210633

60.924

100

0.609
  pilB Vibrio campbellii strain DS40M4

60.391

100

0.604
  pilB Legionella pneumophila strain ERS1305867

50

96.27

0.481
  pilB Acinetobacter baylyi ADP1

48.905

97.336

0.476
  pilB Acinetobacter baumannii D1279779

50.958

92.718

0.472
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.499

92.185

0.364
  pilB Haemophilus influenzae 86-028NP

41.856

86.146

0.361
  pilB Haemophilus influenzae Rd KW20

41.856

86.146

0.361

Multiple sequence alignment