Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOI2P_RS02040 Genome accession   NZ_CP184784
Coordinates   416936..418642 (-) Length   568 a.a.
NCBI ID   WP_412523386.1    Uniprot ID   -
Organism   Shewanella chilikensis strain NTT9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411936..423642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOI2P_RS02010 (ACOI2P_02010) mutT 412499..412897 (-) 399 WP_101055951.1 8-oxo-dGTP diphosphatase MutT -
  ACOI2P_RS02015 (ACOI2P_02015) yacG 412894..413109 (-) 216 WP_039033599.1 DNA gyrase inhibitor YacG -
  ACOI2P_RS02020 (ACOI2P_02020) zapD 413154..413894 (-) 741 WP_165564352.1 cell division protein ZapD -
  ACOI2P_RS02025 (ACOI2P_02025) coaE 413891..414502 (-) 612 WP_101055953.1 dephospho-CoA kinase -
  ACOI2P_RS02030 (ACOI2P_02030) pilD 414503..415417 (-) 915 WP_412503030.1 prepilin peptidase Machinery gene
  ACOI2P_RS02035 (ACOI2P_02035) pilC 415492..416760 (-) 1269 WP_039033595.1 type II secretion system F family protein Machinery gene
  ACOI2P_RS02040 (ACOI2P_02040) pilB 416936..418642 (-) 1707 WP_412523386.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOI2P_RS02045 (ACOI2P_02045) - 418698..419144 (-) 447 WP_412486655.1 pilin -
  ACOI2P_RS02050 (ACOI2P_02050) - 419213..419593 (+) 381 WP_286997984.1 hypothetical protein -
  ACOI2P_RS02055 (ACOI2P_02055) nadC 420157..421017 (-) 861 WP_101055958.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACOI2P_RS02060 (ACOI2P_02060) ampD 421176..421754 (+) 579 WP_342209518.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACOI2P_RS02065 (ACOI2P_02065) ampE 421808..422659 (+) 852 WP_101055959.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62859.87 Da        Isoelectric Point: 5.2480

>NTDB_id=1111224 ACOI2P_RS02040 WP_412523386.1 416936..418642(-) (pilB) [Shewanella chilikensis strain NTT9]
MPTTGLHLGLSTLFIRKGLLNESSLTEAIKQSQNSNQALVTTLVTSKLLSARSIAELCYEEYGTPLLDLNEFDLSSIPDE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKVLEEDLSSLDIDGLDEES
LAGIEVTDTDKRPEDNSEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYMEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQAEEVPEHELLQLGFS
QQQIDSGFTVYKPCGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSLA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1111224 ACOI2P_RS02040 WP_412523386.1 416936..418642(-) (pilB) [Shewanella chilikensis strain NTT9]
ATGCCCACTACCGGCCTCCACTTAGGATTATCGACGCTATTTATCCGTAAGGGGCTGCTCAATGAGAGCAGCCTGACGGA
GGCCATCAAGCAGTCGCAAAACAGCAACCAAGCCCTGGTCACCACCCTGGTGACCAGCAAGCTTTTATCTGCCCGCAGCA
TAGCCGAGCTCTGTTATGAGGAGTACGGCACCCCGCTGCTGGATCTCAATGAGTTTGACCTCAGCAGCATTCCGGATGAG
TTTCTCAACAAGAAACTGATCGACAAACACAGATGTCTGCCGCTGTTCAAGCGCGGCAACCGCCTCTATATCGCCACCTC
GGATCCCACCAATATCGCCGCCCTGGAAGACTTTCAGTTCAGCGCCGGCCTGCATGCCGAGGCGATACTGGTCGAAGAGG
ACAAGTTAGCCAAGGCGCTGGAAAAGGTACTGGAAGAGGACTTGAGCTCGCTGGATATCGATGGCCTGGATGAAGAGTCC
CTCGCCGGCATTGAAGTCACAGATACCGACAAGCGCCCGGAAGACAACAGCGAAGGCAAGGATGACGCCCCCATCGTCAT
CTATATCAATAAAATTCTCACCGATGCCATCCGTAAGGGCGCATCGGATCTGCATTTTGAACCCTACGAGAAGCGCTACC
GCATCCGTTTTCGTATCGATGGCATACTCCATGAGGTGTCCGAGCCGCCGATTTCACTTTCCGGACGGATATCGGCTCGC
TTAAAGGTGATGTCCAAGCTGGATATCGCCGAGCGCCGGGTGCCCCAGGATGGCCGTATCAAGATGCGGCTGTCGCGCAA
CAAGAGCATCGACTTTCGGGTCAGTACCCTGCCTACCCTCTGGGGCGAAAAGATAGTGATGCGGATCCTCGACTCCTCTT
CGGCGCAGTTGGGCATAGAAAAGCTCGGCTATGAGGAAGACCAGAAACAAGCCTATATGGAGATGCTGGCAAGACCCCAG
GGGATGATCCTGGTTACTGGCCCCACGGGTTCGGGGAAAACCGTGTCCCTCTATACCGGCCTCAATATTCTCAATACCGA
GGAGCGCAATATCAGCACCGCCGAAGATCCGGTGGAGATTAACCTCGAAGGGGTTAACCAGGTGCATATCAACCCCAAGG
CCGGGTTAACCTTCGCCTCGGCGCTGCGCTCCTTTCTGCGTCAGGATCCGGACGTGGTGATGGTGGGGGAAATTCGTGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCCCAAACCGGCCACTTGGTGCTCTCGACCCTGCACACCAACTCATCGGC
CGAAACCCTCACCCGTTTGCTCAATATGGGCGTACCCGGCTACAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGTCTCTGCCCCGAATGCCGACAAGCGGAAGAGGTCCCCGAACATGAACTGCTGCAGCTGGGCTTCAGC
CAGCAACAGATAGATAGCGGTTTTACCGTTTATAAGCCATGCGGCTGTGAGCACTGCTCCGGCGGTTACAAGGGCCGAGT
CGGTATCTATGAAGTGATGAAGATGTCCGATGAAATTGCCAGAACCATTATGGAAGGCGGTAATTCACTGCAGATTGCCG
CTACCGCCAAGCAGCAGGGGATGAGGGACTTACGCCTGTCTGGCCTGCTTAAAGTCATTCAGGGCGTCACCAGCCTTGCC
GAGGTCAACCGGGTAACCAGTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.002

98.768

0.583
  pilB Acinetobacter baumannii D1279779

58.111

98.768

0.574
  pilB Legionella pneumophila strain ERS1305867

55.477

99.648

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

52.982

100

0.532
  pilB Vibrio cholerae strain A1552

53.506

95.423

0.511
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

48.936

99.296

0.486
  pilF Thermus thermophilus HB27

38.146

98.768

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.309

97.887

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

41.483

87.852

0.364

Multiple sequence alignment