Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACODUA_RS03305 Genome accession   NZ_CP184748
Coordinates   744107..745327 (+) Length   406 a.a.
NCBI ID   WP_014147180.1    Uniprot ID   G4T0U4
Organism   Methylotuvimicrobium alcaliphilum 20Z     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739107..750327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACODUA_RS03295 (ACODUA_03295) - 739460..742270 (+) 2811 WP_014147178.1 valine--tRNA ligase -
  ACODUA_RS03300 (ACODUA_03300) pilB 742386..744104 (+) 1719 WP_014147179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACODUA_RS03305 (ACODUA_03305) pilC 744107..745327 (+) 1221 WP_014147180.1 type II secretion system F family protein Machinery gene
  ACODUA_RS03310 (ACODUA_03310) pilD 745713..746615 (+) 903 WP_014147181.1 prepilin peptidase Machinery gene
  ACODUA_RS03315 (ACODUA_03315) coaE 746602..747225 (+) 624 WP_084685285.1 dephospho-CoA kinase -
  ACODUA_RS03320 (ACODUA_03320) zapD 747229..747999 (+) 771 WP_014147183.1 cell division protein ZapD -
  ACODUA_RS03325 (ACODUA_03325) yacG 747999..748205 (+) 207 WP_014147184.1 DNA gyrase inhibitor YacG -
  ACODUA_RS03330 (ACODUA_03330) - 748833..749255 (-) 423 WP_014147186.1 hypothetical protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44944.00 Da        Isoelectric Point: 6.8775

>NTDB_id=1111131 ACODUA_RS03305 WP_014147180.1 744107..745327(+) (pilC) [Methylotuvimicrobium alcaliphilum 20Z]
MSEQIEQIDFIWDGKDKSGKRTKGEIAAKSETIARSELRKMGVRVISVKKKPKPLFGARVKAITPGDIAIFSRQLATMLE
AGVPLVQSFDIIGRGHENPSMQKMLLDIKTDIEGGDTLAEALKRRPVYFDELFCNLVESGEQAGVLETLLDKIATYKEKT
ESLKKKIKKALTYPIAVLVVAFVVTAILLIFVVPVFEELFQSFGADLPAFTKMVINLSEWMQEWWWIIVLSIVAIVYVFG
YFHKRSRPFNHFLDRTLLKIPVVGLILNKSAIARFARTLSTMSAAGVPLVEALESVAGACGNIIYADAVLKMREDVATGQ
RLQFAMQQTVLFPHMVTQMVAIGEESGSIDTMLSKVADFYEEEVDNLVDNLSSLMEPLIMSVLGVLVGGLIVAMYLPIFK
MGAAVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1111131 ACODUA_RS03305 WP_014147180.1 744107..745327(+) (pilC) [Methylotuvimicrobium alcaliphilum 20Z]
ATGTCTGAACAAATCGAACAAATTGATTTCATCTGGGATGGTAAAGACAAAAGCGGGAAACGCACCAAGGGGGAAATCGC
CGCGAAATCCGAAACGATTGCTCGGAGCGAATTGCGCAAAATGGGCGTGCGCGTCATTAGTGTGAAGAAAAAACCCAAAC
CCTTGTTCGGCGCGCGTGTCAAAGCCATTACGCCAGGCGATATCGCTATTTTTAGCCGTCAACTCGCGACGATGCTCGAA
GCCGGCGTGCCGTTGGTGCAATCGTTCGATATCATCGGGAGAGGTCATGAAAACCCGAGCATGCAAAAAATGCTGCTTGA
TATCAAGACGGATATCGAAGGCGGCGATACCTTGGCCGAAGCGCTGAAAAGAAGACCGGTCTATTTCGATGAGCTTTTTT
GTAATTTGGTCGAGTCGGGCGAACAGGCCGGGGTGCTTGAGACATTGCTCGATAAGATAGCGACTTATAAAGAAAAAACC
GAATCGCTCAAGAAAAAAATTAAAAAAGCGTTGACCTACCCGATTGCGGTGCTGGTCGTCGCGTTTGTCGTCACCGCCAT
TTTGCTGATTTTCGTCGTGCCGGTGTTCGAAGAGTTGTTTCAGAGCTTCGGAGCCGATCTTCCCGCTTTCACGAAGATGG
TTATCAACCTTTCCGAATGGATGCAAGAGTGGTGGTGGATCATCGTCCTCAGTATTGTGGCAATCGTTTATGTTTTTGGT
TATTTCCATAAACGCTCAAGACCGTTCAATCATTTTCTGGACAGAACCTTATTGAAAATACCGGTTGTCGGATTGATCTT
GAATAAATCAGCCATCGCACGCTTCGCCAGAACGTTGTCGACGATGTCCGCCGCCGGCGTTCCGTTAGTTGAAGCGCTGG
AGTCTGTTGCGGGTGCTTGCGGAAACATTATTTATGCCGATGCTGTGTTAAAGATGCGCGAAGATGTCGCGACCGGCCAA
CGCTTGCAATTTGCCATGCAGCAAACCGTTTTGTTCCCCCACATGGTCACCCAAATGGTCGCAATCGGTGAGGAATCCGG
TTCGATAGACACGATGCTGAGTAAAGTTGCCGATTTTTACGAAGAAGAGGTCGATAATTTGGTAGACAATCTCAGCAGCT
TGATGGAGCCGTTGATCATGTCGGTGTTGGGTGTCTTGGTTGGCGGCTTGATCGTTGCGATGTACCTGCCGATCTTTAAA
ATGGGTGCGGCAGTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4T0U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

61.307

98.03

0.601
  pilC Pseudomonas stutzeri DSM 10701

58.586

97.537

0.571
  pilC Acinetobacter baumannii D1279779

58.186

97.783

0.569
  pilC Acinetobacter baylyi ADP1

56.171

97.783

0.549
  pilG Neisseria meningitidis 44/76-A

45.75

98.522

0.451
  pilG Neisseria gonorrhoeae MS11

45.5

98.522

0.448
  pilC Vibrio campbellii strain DS40M4

41.457

98.03

0.406
  pilC Vibrio cholerae strain A1552

40.955

98.03

0.401
  pilC Thermus thermophilus HB27

38.191

98.03

0.374

Multiple sequence alignment