Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACODT9_RS03300 Genome accession   NZ_CP184747
Coordinates   742440..744158 (+) Length   572 a.a.
NCBI ID   WP_014147179.1    Uniprot ID   G4T0U3
Organism   Methylotuvimicrobium alcaliphilum strain 20ZK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 737440..749158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACODT9_RS03285 (ACODT9_03285) - 737520..739007 (+) 1488 WP_014147176.1 leucyl aminopeptidase -
  ACODT9_RS03290 (ACODT9_03290) - 739019..739447 (+) 429 WP_014147177.1 DNA polymerase III subunit chi -
  ACODT9_RS03295 (ACODT9_03295) - 739514..742324 (+) 2811 WP_014147178.1 valine--tRNA ligase -
  ACODT9_RS03300 (ACODT9_03300) pilB 742440..744158 (+) 1719 WP_014147179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACODT9_RS03305 (ACODT9_03305) pilC 744161..745381 (+) 1221 WP_014147180.1 type II secretion system F family protein Machinery gene
  ACODT9_RS03310 (ACODT9_03310) pilD 745767..746669 (+) 903 WP_014147181.1 prepilin peptidase Machinery gene
  ACODT9_RS03315 (ACODT9_03315) coaE 746674..747279 (+) 606 WP_014147182.1 dephospho-CoA kinase -
  ACODT9_RS03320 (ACODT9_03320) zapD 747283..748053 (+) 771 WP_014147183.1 cell division protein ZapD -
  ACODT9_RS03325 (ACODT9_03325) yacG 748053..748259 (+) 207 WP_014147184.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62845.67 Da        Isoelectric Point: 5.9203

>NTDB_id=1111108 ACODT9_RS03300 WP_014147179.1 742440..744158(+) (pilB) [Methylotuvimicrobium alcaliphilum strain 20ZK]
MATDLIDLPIGGLPKCLVQAGLITEADMQLHMQAARKAKLPLISYLVTNKLVDSKSVTAKASKEFGIPQLDLDALDIRSL
PVNLVSEKLIRKHHALPLLKRGNRLFLAVSDPGNFQAQDEIKFHTRLNIDTILVEEDKLSKAIETSLEAADTTMTDLLDA
DLENLEITGGDQEQAGDEVASDVDDAPIVRFVNKILLDSIKKGVSDIHLEPYEKSFRIRFRADGMLYEVASPPPNISTRI
ISRIKVMAKMDIAERRVPQDGRIKMALSKNRAIDFRVNSCPTLFGEKVVLRILDPTSAQIGIEKLGFEPVQQQRFLEAIN
RPYGMVLVTGPTGSGKTVTLYTGLNLLNSVERNISTAEDPVEITVPGINQVNMNPKAGLTFAGALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLVQMGVPPFNIVSAVHLIMAQRLARRLCEYCKVKSDLPDSVLISA
GFKEEDLGKMTIFTANPNGCEHCTSGFKGRVGIYQVMPISENMRKLILEGGNTMQLAEQAKAEGINDLRASGLEKVCQGI
TSLEEIDRVTKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1111108 ACODT9_RS03300 WP_014147179.1 742440..744158(+) (pilB) [Methylotuvimicrobium alcaliphilum strain 20ZK]
ATGGCAACAGATTTAATCGACCTGCCGATCGGCGGGTTGCCGAAATGTTTGGTACAAGCCGGCCTGATTACCGAGGCGGA
TATGCAGTTGCATATGCAGGCAGCGAGGAAAGCTAAATTACCGCTGATTAGTTATTTAGTGACTAACAAGCTAGTGGATA
GCAAATCCGTAACCGCCAAAGCTTCCAAAGAGTTCGGTATTCCGCAGCTCGATCTCGATGCGCTCGATATTCGTAGCTTG
CCGGTCAATTTAGTCAGCGAAAAGCTGATTAGAAAGCATCATGCTTTACCTTTGCTTAAGCGCGGAAACCGGTTGTTTTT
GGCGGTATCCGACCCCGGCAATTTTCAAGCTCAGGACGAGATAAAGTTTCATACGCGCCTGAATATCGATACGATTCTGG
TCGAAGAAGATAAGTTGTCCAAGGCGATCGAGACTTCGCTGGAAGCCGCCGATACCACGATGACCGATTTGCTGGATGCC
GACCTGGAGAATTTAGAAATTACCGGGGGCGATCAGGAGCAAGCTGGAGACGAGGTAGCGAGTGATGTGGACGATGCGCC
AATCGTCCGTTTCGTTAATAAAATTTTGCTCGATTCGATTAAAAAAGGGGTTTCGGATATTCATCTTGAACCTTATGAAA
AAAGCTTTCGAATCCGCTTTCGCGCAGACGGCATGCTCTATGAAGTGGCAAGTCCTCCTCCCAATATCTCGACCCGGATT
ATTTCCCGAATCAAGGTCATGGCTAAAATGGATATCGCAGAACGGCGCGTTCCCCAAGACGGGCGGATTAAGATGGCGTT
GTCGAAAAATCGGGCAATCGATTTCCGGGTTAATAGTTGTCCGACGCTATTCGGCGAAAAAGTCGTATTGCGTATTCTCG
ATCCAACCAGCGCGCAAATCGGCATCGAAAAACTCGGCTTTGAACCCGTCCAACAACAACGGTTTCTTGAGGCGATTAAC
CGGCCTTACGGCATGGTTTTGGTAACAGGGCCTACCGGTAGCGGTAAAACTGTTACCCTTTACACAGGCTTGAATCTGCT
TAATAGCGTCGAACGGAATATATCGACCGCCGAGGACCCGGTTGAAATTACGGTGCCGGGTATCAATCAGGTTAATATGA
ATCCGAAAGCCGGGTTGACCTTTGCCGGTGCCTTGCGGGCTTTTCTTCGACAAGATCCGGATATCATCATGGTCGGTGAG
ATACGGGATTTGGAAACCGCCGAAATTGCCGTCAAGGCCGCACAAACGGGCCACTTGGTATTGTCCACGCTGCATACTAA
CGATGCGCCGCAAACGTTAAATCGGCTCGTGCAAATGGGCGTGCCGCCGTTTAACATCGTTTCGGCGGTGCATTTGATCA
TGGCTCAACGCCTCGCTAGACGCTTATGCGAATATTGTAAGGTCAAATCCGATTTACCGGATTCGGTATTGATTTCGGCC
GGTTTCAAGGAAGAGGATCTAGGAAAAATGACAATTTTCACGGCCAATCCTAACGGTTGCGAACATTGCACTAGCGGCTT
CAAAGGCCGGGTCGGCATTTATCAGGTTATGCCGATCAGCGAAAATATGCGGAAACTGATTCTGGAAGGCGGCAATACCA
TGCAGTTGGCCGAACAAGCCAAAGCCGAAGGCATCAACGACCTACGCGCGTCCGGTCTTGAAAAAGTCTGTCAAGGCATC
ACTAGCCTGGAAGAAATCGACCGGGTCACTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4T0U3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.27

98.601

0.565
  pilB Acinetobacter baumannii D1279779

53.191

98.601

0.524
  pilB Acinetobacter baylyi ADP1

52.743

98.776

0.521
  pilB Vibrio cholerae strain A1552

48.221

98.252

0.474
  pilF Neisseria gonorrhoeae MS11

47.434

98.776

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

47.772

98.077

0.469
  pilB Vibrio campbellii strain DS40M4

47.237

98.077

0.463
  pilF Thermus thermophilus HB27

38.947

99.65

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

42.115

90.909

0.383

Multiple sequence alignment