Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACODT9_RS03310 Genome accession   NZ_CP184747
Coordinates   745767..746669 (+) Length   300 a.a.
NCBI ID   WP_014147181.1    Uniprot ID   -
Organism   Methylotuvimicrobium alcaliphilum strain 20ZK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 740767..751669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACODT9_RS03300 (ACODT9_03300) pilB 742440..744158 (+) 1719 WP_014147179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACODT9_RS03305 (ACODT9_03305) pilC 744161..745381 (+) 1221 WP_014147180.1 type II secretion system F family protein Machinery gene
  ACODT9_RS03310 (ACODT9_03310) pilD 745767..746669 (+) 903 WP_014147181.1 prepilin peptidase Machinery gene
  ACODT9_RS03315 (ACODT9_03315) coaE 746674..747279 (+) 606 WP_014147182.1 dephospho-CoA kinase -
  ACODT9_RS03320 (ACODT9_03320) zapD 747283..748053 (+) 771 WP_014147183.1 cell division protein ZapD -
  ACODT9_RS03325 (ACODT9_03325) yacG 748053..748259 (+) 207 WP_014147184.1 DNA gyrase inhibitor YacG -
  ACODT9_RS03330 (ACODT9_03330) - 748887..749240 (-) 354 WP_223842343.1 hypothetical protein -
  ACODT9_RS03335 (ACODT9_03335) - 749309..751063 (-) 1755 WP_014147187.1 Hsp70 family protein -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 33248.29 Da        Isoelectric Point: 5.9904

>NTDB_id=1111110 ACODT9_RS03310 WP_014147181.1 745767..746669(+) (pilD) [Methylotuvimicrobium alcaliphilum strain 20ZK]
MQGLEIFADSPAALMALTGVIGLMVGSFLNVVIYRLPIMMQKSWRRECIDYLQSTDDIRCAELDTESGEADSKFNLVFPL
SRCPHCETPIKPYQNIPVLSYLLLKGRCAQCNTPISKRYPVIEGLTGFFSVLIAWHFGYSVEALFALILTWSLIALSAID
IDHQLLPDSITLPLLWLGLFLSLFDIFIDTHSSIIGAIAGYLSLWTVYHGFKLLTGKEGMGYGDFKLLAMFGAWLGWQYI
LPIVLLSSLVGAVTGIAMVILVKRDKNIPIPFGPYLAAAGWIALLWGDKINQAYLNFAGL

Nucleotide


Download         Length: 903 bp        

>NTDB_id=1111110 ACODT9_RS03310 WP_014147181.1 745767..746669(+) (pilD) [Methylotuvimicrobium alcaliphilum strain 20ZK]
ATGCAAGGACTTGAAATATTCGCCGATTCGCCGGCTGCACTAATGGCTTTAACGGGTGTTATTGGTCTCATGGTCGGTAG
TTTTCTGAATGTCGTCATTTACCGTCTGCCGATTATGATGCAAAAAAGTTGGCGCCGCGAGTGCATCGATTATCTGCAGT
CGACGGATGATATACGGTGTGCGGAGCTCGATACAGAAAGTGGGGAGGCCGACTCAAAATTTAACTTAGTCTTTCCATTG
TCGCGTTGTCCGCACTGCGAAACCCCGATAAAGCCTTATCAAAATATTCCGGTACTCAGTTACTTGTTGTTGAAAGGCCG
TTGTGCTCAATGCAATACGCCGATATCGAAACGCTATCCCGTTATCGAGGGATTGACCGGTTTTTTTTCGGTTTTAATCG
CCTGGCATTTCGGTTATTCGGTAGAGGCGCTATTCGCGCTCATACTTACCTGGTCCTTGATTGCGCTGAGTGCGATCGAT
ATTGACCATCAACTTTTGCCGGATTCGATTACGTTACCTTTACTTTGGTTGGGGTTGTTTTTAAGCCTGTTTGATATCTT
CATCGATACGCATTCCTCGATTATCGGTGCTATCGCCGGTTATTTATCGCTTTGGACGGTTTATCATGGTTTCAAGCTGT
TGACCGGCAAAGAGGGCATGGGATACGGCGACTTCAAACTGTTGGCAATGTTTGGGGCATGGTTGGGTTGGCAATATATC
CTGCCGATCGTTTTACTGTCATCCTTGGTCGGTGCCGTTACCGGCATCGCGATGGTTATTTTGGTAAAGCGCGACAAGAA
TATTCCAATTCCATTCGGCCCTTATTTGGCCGCGGCCGGTTGGATCGCTTTACTGTGGGGCGATAAAATCAATCAGGCTT
ATCTCAATTTTGCCGGATTATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-137)

Type IV leader peptidase family (Peptidase_A24)

(147-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.676

98.667

0.5
  pilD Vibrio campbellii strain DS40M4

49.32

98

0.483
  pilD Acinetobacter baumannii D1279779

50.173

96.333

0.483
  pilD Acinetobacter nosocomialis M2

50.173

96.333

0.483
  pilD Neisseria gonorrhoeae MS11

47.902

95.333

0.457

Multiple sequence alignment