Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACOH25_RS23530 Genome accession   NZ_CP184738
Coordinates   5256593..5257762 (+) Length   389 a.a.
NCBI ID   WP_331693087.1    Uniprot ID   -
Organism   Pandoraea sputorum strain whet-A341     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5251593..5262762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOH25_RS23500 cgtA 5251698..5252828 (-) 1131 WP_331693088.1 Obg family GTPase CgtA -
  ACOH25_RS23505 rpmA 5252978..5253238 (-) 261 WP_039393311.1 50S ribosomal protein L27 -
  ACOH25_RS23510 rplU 5253267..5253578 (-) 312 WP_039393314.1 50S ribosomal protein L21 -
  ACOH25_RS23515 - 5253906..5254835 (+) 930 WP_331693288.1 polyprenyl synthetase family protein -
  ACOH25_RS23525 - 5255159..5256451 (+) 1293 WP_039393320.1 HlyC/CorC family transporter -
  ACOH25_RS23530 pilF 5256593..5257762 (+) 1170 WP_331693087.1 GspE/PulE family protein Machinery gene
  ACOH25_RS23535 - 5257901..5259127 (+) 1227 WP_331693086.1 type II secretion system F family protein -
  ACOH25_RS23540 - 5259124..5259984 (+) 861 WP_331693085.1 A24 family peptidase -
  ACOH25_RS23545 coaE 5259981..5260595 (+) 615 WP_331693083.1 dephospho-CoA kinase -
  ACOH25_RS23550 zapD 5260771..5261526 (+) 756 WP_039393327.1 cell division protein ZapD -
  ACOH25_RS23555 yacG 5261531..5261725 (+) 195 WP_039393329.1 DNA gyrase inhibitor YacG -
  ACOH25_RS23560 - 5261862..5262296 (-) 435 WP_150777649.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 41824.02 Da        Isoelectric Point: 7.1647

>NTDB_id=1111011 ACOH25_RS23530 WP_331693087.1 5256593..5257762(+) (pilF) [Pandoraea sputorum strain whet-A341]
MLRPTIASDDALPSTGTPASTTPLQRLEQILSEAIRAGASDIHFEPMAQRFRVRLRVDGRLQEHGDVPLTARDMLVSRLK
VLANLDIAQKRLPQDGRMVWREAGEPVECRVSALPTLHGEKLVVRLLDGAKVPLSLEGLGYSPKQYLALTQAIARPHGLI
LLTGPTGSGKTVSLYSCLRRLNDASRNIVTIEDPVEIRLAGITQVNLNERAGLDFATALRAFLRQDPDVLVVGEIRDAQT
AEIAIQAAQTGHLVFATVHANDAPSTLARLFDLGVAPFNLSSTLLLVSAQRLLRRRCPAGCEPSRTTTETGLADTCMRCH
GTGFAGRIGAFQVMPISATQAINIAQARSPHELAAQACSEGVMTLREAGLWHVDAGAVAQEDVDATLPA

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=1111011 ACOH25_RS23530 WP_331693087.1 5256593..5257762(+) (pilF) [Pandoraea sputorum strain whet-A341]
ATGCTGCGTCCAACCATCGCCTCCGACGACGCTCTGCCGTCCACCGGCACCCCGGCGTCCACCACACCTCTGCAACGCCT
TGAGCAAATACTGAGCGAAGCGATTCGTGCGGGCGCTTCCGATATCCATTTCGAACCGATGGCGCAGCGCTTTCGGGTCC
GTCTGCGTGTCGACGGTCGCCTGCAGGAGCATGGCGACGTTCCGCTGACAGCGCGCGACATGCTGGTCTCGCGTCTGAAG
GTGCTTGCCAATCTCGACATCGCACAAAAGCGCTTGCCGCAGGACGGACGCATGGTGTGGCGCGAAGCTGGTGAACCGGT
CGAGTGTCGCGTGAGTGCGCTTCCCACCCTGCACGGCGAGAAACTCGTGGTGCGCCTGCTCGATGGCGCGAAAGTCCCTC
TCTCGCTTGAGGGTCTCGGCTATTCGCCGAAGCAATACCTCGCACTCACGCAAGCGATTGCCCGGCCACATGGCCTGATC
TTGCTGACGGGCCCAACGGGCAGTGGAAAGACCGTCTCGCTCTATAGTTGCCTGCGACGTCTGAACGACGCGTCGCGCAA
CATCGTCACCATCGAGGACCCGGTGGAAATTCGCTTGGCCGGTATCACGCAGGTCAATCTCAACGAACGCGCCGGCCTCG
ACTTTGCAACGGCTTTACGTGCGTTTCTCCGTCAGGATCCAGATGTCCTCGTCGTGGGCGAAATCCGCGATGCGCAGACC
GCCGAAATCGCCATACAGGCCGCGCAGACCGGTCACCTTGTGTTCGCCACCGTTCACGCCAACGACGCGCCGAGCACGCT
CGCGCGGCTCTTCGATCTCGGTGTCGCCCCGTTCAATCTGTCGTCGACATTACTGCTGGTCAGCGCACAGCGTCTGCTTA
GGCGACGATGCCCGGCCGGGTGCGAGCCGTCCCGCACGACGACTGAAACAGGCCTCGCTGACACCTGCATGCGGTGTCAC
GGCACCGGTTTTGCCGGACGCATCGGCGCGTTTCAGGTGATGCCGATCAGCGCCACGCAGGCTATCAACATTGCACAGGC
GCGCAGTCCGCACGAGCTGGCGGCGCAGGCATGCAGCGAAGGCGTCATGACGCTGCGAGAAGCCGGGTTATGGCATGTCG
ATGCAGGGGCTGTCGCTCAGGAGGATGTCGATGCGACGCTGCCCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(29-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.462

100

0.486
  pilB Vibrio cholerae strain A1552

47.545

99.486

0.473
  pilB Vibrio campbellii strain DS40M4

47.709

95.373

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

46.194

97.943

0.452
  pilB Acinetobacter baumannii D1279779

43.89

100

0.452
  pilB Legionella pneumophila strain ERS1305867

43.035

100

0.445
  pilB Acinetobacter baylyi ADP1

45.526

97.686

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

40.587

100

0.427
  pilF Thermus thermophilus HB27

41.327

100

0.416
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.114

96.915

0.398
  pilB Haemophilus influenzae 86-028NP

42.265

93.059

0.393
  pilB Haemophilus influenzae Rd KW20

46.602

79.434

0.37
  pilB Glaesserella parasuis strain SC1401

39.024

94.859

0.37

Multiple sequence alignment