Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACOC81_RS24995 Genome accession   NZ_CP184649
Coordinates   5554308..5556407 (-) Length   699 a.a.
NCBI ID   WP_162999985.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain p21.B10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5549308..5561407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOC81_RS24980 (ACOC81_24980) - 5550918..5552546 (-) 1629 WP_122414037.1 SPOR domain-containing protein -
  ACOC81_RS24985 (ACOC81_24985) aroB 5552556..5553659 (-) 1104 WP_122421148.1 3-dehydroquinate synthase -
  ACOC81_RS24990 (ACOC81_24990) aroK 5553785..5554303 (-) 519 WP_004882714.1 shikimate kinase AroK -
  ACOC81_RS24995 (ACOC81_24995) pilQ 5554308..5556407 (-) 2100 WP_162999985.1 type IV pilus secretin PilQ Machinery gene
  ACOC81_RS25000 (ACOC81_25000) - 5556455..5556982 (-) 528 WP_029243168.1 pilus assembly protein PilP -
  ACOC81_RS25005 (ACOC81_25005) pilO 5556979..5557602 (-) 624 WP_069143009.1 type 4a pilus biogenesis protein PilO -
  ACOC81_RS25010 (ACOC81_25010) - 5557599..5558177 (-) 579 WP_025993896.1 PilN domain-containing protein -
  ACOC81_RS25015 (ACOC81_25015) pilM 5558177..5559241 (-) 1065 WP_122421621.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75357.76 Da        Isoelectric Point: 5.0501

>NTDB_id=1110576 ACOC81_RS24995 WP_162999985.1 5554308..5556407(-) (pilQ) [Pseudomonas viridiflava strain p21.B10]
MTRILSIIGVSLWIAMMSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPTTAKGAARVAVPPPTPASARPYVPA
GVRAIRNVDFQRGELGEGNVVIDLSNASIAADIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVSATASGDKASI
SIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKQIGVRWGGRTDRSRKW
SVGGLDDNGDEAGNTGNNLTANIPFVDLGAPDATAGVGIGFLTNNALLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDVVAEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=1110576 ACOC81_RS24995 WP_162999985.1 5554308..5556407(-) (pilQ) [Pseudomonas viridiflava strain p21.B10]
ATGACCCGGATTCTCTCGATTATCGGCGTTTCGTTATGGATAGCGATGATGTCGCCGGTTCTCCAGGCCGCCAATCTCAA
GACGCTCGACGTCGCGGCCCTGCCGGGTGATCGAATCGAGCTCAAGCTTTCCTTCGACGCTCCGGTGCCCGCGCCACGCG
GCTATACCACCGAGCAGCCTGCGCGCATTGCGCTGGACCTGCCGGGCGTGACCAGCCAGCTGGCGACCAAGAGTCGTGAT
CTGGGCGCAGGCAATGCCCGCAGTGTGGTGGTTGTGCAGGCGCAGGACCGTACGCGAGTCATCATCAACCTGACGACGCT
TTCGCCGTACAGCTCACGCGTCGATGGCAACAACTTGTTCGTGGTCATCGGTCAGGGTGCGGCGGCGGCACAGGCTTCGC
AGCCGACCACCGCCAAGGGCGCTGCACGGGTCGCCGTGCCGCCGCCCACACCGGCATCGGCGCGGCCCTACGTGCCTGCG
GGCGTCAGAGCCATTCGCAATGTTGACTTCCAGCGCGGCGAGCTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAATGC
CAGCATCGCTGCGGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACGCAATTGCCCGATCCGCTGC
GCGTGCGTCTGGACGTGAAAGACTTTGCCACACCCGTGCAATTCGTCAGCGCCACAGCGTCCGGCGACAAGGCCAGCATT
TCCATCGAGCCATCGGGTGCGTTCGATTATTCGGCGTTCCAGACCGAAAACAAACTGACCATCAGCGTGCGCCCATTGAC
CAATGAGGACGTGGATCGTCGCGCCGCCGACAAGCCGGTCTACACCGGCGAGAAGCTTTCGCTGAATTTCCAGGACATCG
ATGTGCGTTCGGTACTGCAGCTGATCGCCGATTTCACCAACCTCAATCTGGTTGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGTCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGT
CGGCAGCGTGTTGCTGGTCGCCCCGGCTGACGAAATCGCCGCGCGTGAGCGTCAGGAACTGGAGTCGCTCAAGCAGATTG
CCGAACTGGCCCCGTTGCGTCGTGAGTTGCTGCAGGTCAACTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCG
GTCACCAGCGCCGAGTCCAAGGCGGACGAGCGTGGTTCCATTACTGTGGATGAGCGCACCAACAACATCATCGCCTACCA
GACTCAGGATCGACTCGACGAGCTGCGCCGCATCGTCTCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAAGCGC
GAATCGTTGAAGCCAACGTCGACTATGACAAGCAGATCGGCGTGCGCTGGGGCGGGCGAACCGACCGGTCGCGCAAGTGG
AGTGTCGGCGGCCTTGACGATAACGGCGATGAAGCAGGCAATACGGGCAACAATCTGACGGCCAACATTCCGTTTGTGGA
TCTGGGTGCTCCAGATGCCACGGCGGGTGTCGGGATCGGTTTCCTGACCAACAATGCACTGCTGGACCTTGAGCTGAGTG
CGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAGACTGCCAAGATCCTC
AAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCCACGACAGTGAGCTTCAAGGAGGCGTCCCTGTCGCT
TGAGGTCACGCCACAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCTGATTACCTCA
ATGCGGTGCTGGGCGTGCCTCCTATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACCATCGTG
ATTGGCGGGGTGTTCTCCAATACGCAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCAGGCGCGACGTGGTGGCGGAGGCGAAATCCGAGCTGTTGGTATTTCTGACACCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(534-690)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.399

100

0.747
  comQ Acinetobacter baylyi ADP1

40.799

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.756

100

0.395
  pilQ Acinetobacter baumannii D1279779

37.756

100

0.395
  pilQ Legionella pneumophila strain ERS1305867

38.012

97.854

0.372
  pilQ Legionella pneumophila strain Lp02

38.067

97.711

0.372

Multiple sequence alignment