Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACOC81_RS25015 Genome accession   NZ_CP184649
Coordinates   5558177..5559241 (-) Length   354 a.a.
NCBI ID   WP_122421621.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain p21.B10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5553177..5564241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOC81_RS24990 (ACOC81_24990) aroK 5553785..5554303 (-) 519 WP_004882714.1 shikimate kinase AroK -
  ACOC81_RS24995 (ACOC81_24995) pilQ 5554308..5556407 (-) 2100 WP_162999985.1 type IV pilus secretin PilQ Machinery gene
  ACOC81_RS25000 (ACOC81_25000) - 5556455..5556982 (-) 528 WP_029243168.1 pilus assembly protein PilP -
  ACOC81_RS25005 (ACOC81_25005) pilO 5556979..5557602 (-) 624 WP_069143009.1 type 4a pilus biogenesis protein PilO -
  ACOC81_RS25010 (ACOC81_25010) - 5557599..5558177 (-) 579 WP_025993896.1 PilN domain-containing protein -
  ACOC81_RS25015 (ACOC81_25015) pilM 5558177..5559241 (-) 1065 WP_122421621.1 pilus assembly protein PilM Machinery gene
  ACOC81_RS25020 (ACOC81_25020) - 5559451..5561892 (+) 2442 WP_407072029.1 penicillin-binding protein 1A -
  ACOC81_RS25025 (ACOC81_25025) - 5562289..5562744 (+) 456 WP_004882721.1 YkgJ family cysteine cluster protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38058.48 Da        Isoelectric Point: 4.7090

>NTDB_id=1110577 ACOC81_RS25015 WP_122421621.1 5558177..5559241(-) (pilM) [Pseudomonas viridiflava strain p21.B10]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVVGKAKTNARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSLRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1110577 ACOC81_RS25015 WP_122421621.1 5558177..5559241(-) (pilM) [Pseudomonas viridiflava strain p21.B10]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGTTCCACCTCGGTCAAGCTCCTTGAATT
GAGTCGTTCCGGAACCCGCTACAAAGTCGAGTCTTACGCTGTTGAGCCGTTGCCGGCCAATGCCGTCGTCGAGAAGAACA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCACGGGTCGTCGGCAAGGCCAAGACCAACGCCAGAATAGTGGCGGTC
GCTGTCGCAGGCTCCGCAGTCATCACCAAGACGATCGAAATGGATGCCGGTCTCTCGGACGACGACATGGAAAATCAGCT
CAAGCTCGAGGCTGACCAGTACATCCCCTATCCCCTTGAAGAAGTGGCCATCGATTTCGAAGTGCAGGGCTATTCGCTGC
GCAACCCCGAACGCGTGGAAGTCCTTCTGGCTGCCTGTCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCGCTG
GCGGGCCTCACCGCAAAAGTCGTCGACGTCGAAGCCTATGCCCTTGAGCGTTCGTTCGGGCTGCTGGCTGCGCAGCTGGG
CAATGGTCACGACGAACTGACCGTTGCGGTCGTCGATATCGGTGCGACCATGACCACGCTGAGCGTTCTGCACCATGGCC
GGATCATCTACACCCGTGAGCAACTGTTCGGCGGACGCCAACTGACCGAAGAAATCCAGCGCCGCTATGGCCTGTCGATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTACCGGACGACTATGTCAGTGAAGTGCTGGAACCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCGCGCTCACTTCAGTTCTTCTTCGCGGCAGGCCAGTACAACTCTGTGGATCACATCATGCTCG
CTGGCGGTACGGCTTCCATTTCCGGCCTGGAGCATCTGATTCAGCGCCGACTCGGCACGCCGACCCAGGTGGCCAACCCG
TTTGCCGACATGGCCCTCAGTTCCAAGGTCAACGCCGGAGCGCTGGCCAGTGATGCGCCCGCGCTGATGATCGCGTGCGG
TCTTGCTTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.887

100

0.48

Multiple sequence alignment