Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNOHK_RS06815 Genome accession   NZ_CP184332
Coordinates   1613270..1614400 (+) Length   376 a.a.
NCBI ID   WP_075287180.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris isolate BJSJQ20200612-pXX3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1608270..1619400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNOHK_RS06790 (ACNOHK_06790) - 1608541..1609131 (-) 591 WP_029628896.1 M23 family metallopeptidase -
  ACNOHK_RS06795 (ACNOHK_06795) - 1609275..1609697 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ACNOHK_RS06800 (ACNOHK_06800) proC 1610238..1611098 (-) 861 WP_050912119.1 pyrroline-5-carboxylate reductase -
  ACNOHK_RS06805 (ACNOHK_06805) - 1611139..1611822 (-) 684 WP_050912120.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNOHK_RS06810 (ACNOHK_06810) pilT 1612110..1613147 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNOHK_RS06815 (ACNOHK_06815) pilU 1613270..1614400 (+) 1131 WP_075287180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNOHK_RS06820 (ACNOHK_06820) - 1614680..1615354 (+) 675 WP_014508435.1 YitT family protein -
  ACNOHK_RS06825 (ACNOHK_06825) - 1615368..1616267 (-) 900 WP_019237456.1 DUF72 domain-containing protein -
  ACNOHK_RS06830 (ACNOHK_06830) - 1616490..1617752 (-) 1263 WP_075288043.1 hypothetical protein -
  ACNOHK_RS06835 (ACNOHK_06835) - 1617749..1618288 (-) 540 WP_012437807.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.31 Da        Isoelectric Point: 7.0160

>NTDB_id=1108252 ACNOHK_RS06815 WP_075287180.1 1613270..1614400(+) (pilU) [Xanthomonas campestris pv. campestris isolate BJSJQ20200612-pXX3-4]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYKAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1108252 ACNOHK_RS06815 WP_075287180.1 1613270..1614400(+) (pilU) [Xanthomonas campestris pv. campestris isolate BJSJQ20200612-pXX3-4]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGCATGGTGCTGCGGCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACACTGGCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CCACCGGCACCGGCAAGTCGACATCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAATGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGGACCCGCGAAGGCA
TGGACCATGCCATCGCCTTCGCCGAAACCGGGCACCTGGTGCTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATTAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTATAAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTGGATGGCGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment