Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACN19N_RS14030 Genome accession   NZ_CP184160
Coordinates   2919052..2920767 (+) Length   571 a.a.
NCBI ID   WP_269228985.1    Uniprot ID   A0A9E9L583
Organism   Acinetobacter sp. LF10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2914052..2925767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN19N_RS13995 (ACN19N_13995) nusA 2914902..2916386 (-) 1485 WP_005218583.1 transcription termination factor NusA -
  ACN19N_RS14000 (ACN19N_14000) rimP 2916424..2916948 (-) 525 WP_269228983.1 ribosome maturation factor RimP -
  ACN19N_RS14020 (ACN19N_14020) secG 2917574..2917903 (-) 330 WP_005162938.1 preprotein translocase subunit SecG -
  ACN19N_RS14025 (ACN19N_14025) tpiA 2917916..2918707 (-) 792 WP_420001594.1 triose-phosphate isomerase -
  ACN19N_RS14030 (ACN19N_14030) pilB 2919052..2920767 (+) 1716 WP_269228985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN19N_RS14035 (ACN19N_14035) pilC 2920792..2922018 (+) 1227 WP_269228986.1 type II secretion system F family protein Machinery gene
  ACN19N_RS14040 (ACN19N_14040) pilD 2922018..2922878 (+) 861 WP_269228987.1 prepilin peptidase Machinery gene
  ACN19N_RS14045 (ACN19N_14045) coaE 2922880..2923485 (+) 606 WP_269238964.1 dephospho-CoA kinase -
  ACN19N_RS14050 (ACN19N_14050) - 2923482..2924393 (-) 912 WP_269238963.1 DMT family transporter -
  ACN19N_RS14055 (ACN19N_14055) rlmB 2924424..2925173 (-) 750 WP_269238962.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACN19N_RS14060 (ACN19N_14060) - 2925355..2925681 (-) 327 WP_004659291.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63242.53 Da        Isoelectric Point: 5.4233

>NTDB_id=1108025 ACN19N_RS14030 WP_269228985.1 2919052..2920767(+) (pilB) [Acinetobacter sp. LF10]
MSGLHTSPKFSGFIRRLVEDGHMTAANMQNAIDAAKKAKQDIVAHLIEQHRLSPLTIAESISVEFGEPLFDLAVYDVGLL
PRELTDNKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPIIVEHTKLEKLIEQHFGDSGSFDFGDSED
FDLDIDVNQDSPQENDDNSSQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDDQKALFIEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPLNVPKQSLIELG
FTEQDLANPDLQIFQPIGCPECREGYKGRVGIYEVMKVTPAISKIIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1108025 ACN19N_RS14030 WP_269228985.1 2919052..2920767(+) (pilB) [Acinetobacter sp. LF10]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTCATTCGACGTTTAGTTGAAGATGGTCATATGACTGCTGCAAA
TATGCAGAATGCAATTGATGCAGCCAAGAAAGCCAAACAAGATATTGTTGCTCATTTGATTGAACAACATCGTCTTTCTC
CACTTACCATTGCAGAAAGCATCTCCGTAGAATTTGGCGAACCACTTTTTGATTTGGCGGTTTACGATGTTGGATTATTA
CCTCGAGAATTAACCGACAATAAACTCATACAAAAACATCGTGTTGTTCCGTTAATACAACGTGGACAAATTTTGTATGT
TGCTACGAGCAACCCAACCAATATTGATGCATTAGATGCAATCCGGTTCAATACGAAACTTAATATTGAACCAATTATTG
TTGAGCATACGAAACTTGAAAAGTTAATCGAACAACATTTCGGTGATTCAGGTAGTTTCGATTTTGGTGACAGTGAAGAC
TTCGATTTAGATATCGATGTTAATCAAGATTCTCCTCAAGAAAATGACGATAATTCATCTCAAGGTGATGAATCGCCTAT
TGTTAAATATATTAATAAGCTTTTAATTGATGCAATTCGCATGGGCGCATCAGATTTACATTTTGAACCTTACGAAAAAA
CGTATCGTGTACGTTATCGTGTAGATGGCGTTCTACGCCAAATTGCTACCCCACCATTACAAATGGCAAATCGCTTGGCT
TCTCGTTTAAAAGTTATGTCCCAAATGGATATTTCAGAAAAACGAATGCCTCAAGATGGTCGTATTAAACTCAAGTTATC
AAAAACCAAAGCAATTGATTTCCGTGTTAACTCTTTACCTACTTTATTTGGAGAAAAATTAGTACTACGTATTTTGGATC
CATCAAGTGCTATGCTCGGGATTGAAGCATTAGGTTATGAAGATGATCAGAAAGCATTATTCATTGAAGCACTTGAAAAG
CCTCAAGGAATGCTATTGATTACAGGTCCAACAGGTTCTGGTAAAACCGTATCGCTATATACTGGTCTAAATATTCTAAA
CACTGAAGATACCAATATTTCAACAGCAGAAGACCCTGTTGAAATTAACTTGGAAGGCATTAATCAGGTCAACGTGAATC
CAAAGACGGGATTGACTTTCTCTGCTGCATTAAAGGCTTTCTTACGCCAAGACCCGGATGTAATTATGGTCGGTGAGATT
CGTGATTTGGAAACAGCCGAAATTGCAATTAAAGCAGCGCAAACAGGTCATATGGTGATGTCAACACTACATACCAACAG
TGCGCCAGAAACTTTAACTCGTTTACGCAACATGGGTGTACCTTCGTTTAATATCGCAACATCAGTTAATCTTGTTATCG
CACAACGTTTAGCACGACGTTTATGCTCACAATGTAAAATACCTCTTAATGTACCTAAGCAGAGTCTCATTGAACTAGGA
TTTACTGAACAAGATTTAGCCAATCCAGATTTACAAATATTTCAGCCTATTGGATGTCCTGAATGCCGTGAAGGGTATAA
AGGGCGAGTAGGTATTTATGAAGTCATGAAGGTCACCCCTGCAATCTCTAAAATTATCATGGAAGATGGAAATGCACTGC
AAATTGCAGCAGCATCAGAACAAGCAGGGTTTAATAATCTCCGGCGTTCTGGTTTGAAAAAAGTAATGCAAGGAATTACC
TCATTGCAGGAAATTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.487

100

0.825
  pilB Acinetobacter baylyi ADP1

77.058

100

0.771
  pilB Legionella pneumophila strain ERS1305867

53.775

95.096

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.286

98.074

0.483
  pilF Neisseria gonorrhoeae MS11

48.33

99.65

0.482
  pilB Vibrio campbellii strain DS40M4

48.837

97.898

0.478
  pilB Vibrio cholerae strain A1552

51.257

90.543

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

34.804

100

0.373
  pilF Thermus thermophilus HB27

37.5

98.074

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.548

92.995

0.368

Multiple sequence alignment