Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   HZ99_RS12510 Genome accession   NZ_CP008896
Coordinates   2847137..2847547 (-) Length   136 a.a.
NCBI ID   WP_038443435.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain UK4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2842137..2852547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ99_RS12490 (HZ99_12790) nadC 2844183..2845031 (+) 849 WP_038443431.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HZ99_RS12495 (HZ99_12795) - 2845099..2845404 (-) 306 WP_038443433.1 DUF6388 family protein -
  HZ99_RS12505 (HZ99_12805) - 2845653..2847059 (-) 1407 WP_038443434.1 O-antigen ligase family protein -
  HZ99_RS12510 (HZ99_12810) pilA/pilAI 2847137..2847547 (-) 411 WP_038443435.1 pilin Machinery gene
  HZ99_RS12515 (HZ99_12815) pilC 2847751..2848971 (+) 1221 WP_038443437.1 type II secretion system F family protein Machinery gene
  HZ99_RS12520 (HZ99_12820) pilD 2848974..2849846 (+) 873 WP_038443439.1 prepilin peptidase Machinery gene
  HZ99_RS12525 (HZ99_12825) coaE 2849843..2850466 (+) 624 WP_038443440.1 dephospho-CoA kinase -
  HZ99_RS12530 (HZ99_12830) yacG 2850463..2850666 (+) 204 WP_038443442.1 DNA gyrase inhibitor YacG -
  HZ99_RS12535 (HZ99_12835) - 2850663..2850878 (-) 216 WP_038443443.1 hypothetical protein -
  HZ99_RS12540 (HZ99_12840) - 2850942..2851631 (-) 690 WP_038443445.1 energy-coupling factor ABC transporter permease -
  HZ99_RS12545 (HZ99_12845) - 2851628..2852095 (-) 468 WP_038443446.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13828.99 Da        Isoelectric Point: 5.7734

>NTDB_id=110757 HZ99_RS12510 WP_038443435.1 2847137..2847547(-) (pilA/pilAI) [Pseudomonas fluorescens strain UK4]
MRQNGFTLIELLIVVAIIGILATIGLPMYTKHQAKAKFTAGLAEVSALKAGFEDTFNQGTAPTLALIGGTSPTANCVITV
AGDVAAGTGSIACEILDAPAPIQGKFITLARTASEGWKCTATADEQYVAKGCAAGN

Nucleotide


Download         Length: 411 bp        

>NTDB_id=110757 HZ99_RS12510 WP_038443435.1 2847137..2847547(-) (pilA/pilAI) [Pseudomonas fluorescens strain UK4]
ATGCGTCAGAACGGCTTTACTTTGATCGAGTTGTTGATCGTCGTGGCGATCATTGGCATCTTGGCCACCATTGGCTTGCC
CATGTATACCAAGCACCAGGCCAAGGCTAAATTCACGGCGGGGTTGGCGGAAGTCTCGGCGTTGAAGGCCGGCTTCGAAG
ACACCTTCAACCAAGGCACCGCACCCACGCTGGCGCTGATCGGTGGCACCAGCCCGACCGCCAACTGCGTGATCACCGTT
GCGGGTGATGTGGCTGCGGGGACTGGCTCGATTGCCTGCGAAATCCTCGATGCGCCTGCGCCGATCCAGGGCAAGTTCAT
TACCCTGGCGCGCACTGCCAGTGAGGGGTGGAAGTGCACCGCTACCGCTGACGAGCAGTATGTTGCAAAAGGTTGTGCGG
CTGGCAATTGA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(34-132)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.796

100

0.441
  pilA Pseudomonas aeruginosa PAK

41.096

100

0.441
  comP Acinetobacter baylyi ADP1

38.095

100

0.412
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.741

99.265

0.404
  pilA Acinetobacter baumannii strain A118

40.146

100

0.404
  pilA Vibrio cholerae C6706

35.664

100

0.375
  pilA Vibrio cholerae strain A1552

35.664

100

0.375
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.664

100

0.375

Multiple sequence alignment